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Volume 9 Issue 4
Dec.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(4): 388-392

LIU Qiuyu, CHEN Xin, QIN Zongmin, ZHUO Wen, TANG Min. DNA Damage of Cu2+ to Cyprinus carpio[J]. Journal of Tropical Biology, 2018, 9(4): 388-392. doi: 10.15886/j.cnki.rdswxb.2018.04.004
Citation: LIU Qiuyu, CHEN Xin, QIN Zongmin, ZHUO Wen, TANG Min. DNA Damage of Cu2+ to Cyprinus carpio[J]. Journal of Tropical Biology, 2018, 9(4): 388-392. doi: 10.15886/j.cnki.rdswxb.2018.04.004
shu

DNA Damage of Cu2+ to Cyprinus carpio

doi: 10.15886/j.cnki.rdswxb.2018.04.004

  • 1. College of Materials and Chemical Engineering, Hainan University, Haikou, Hainan 570228, China;

  • 2. State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University), Haikou, Hainan 570228, China

  • Received Date: 2018-08-24
  • Rev Recd Date: 2018-10-25
  • Common carp（ Cyprinus carpio） was cultured and exposed to different concentrations of Cu2+,and the DNA damage of the peripheral blood cells of C. carpio was analyzed by using the micronucleus test and the comet assay. The results showed that the rates of comet（ 17. 7%,44. 4%,60. 0%,88. 9%） and micronucleus（ 0. 4‰,8. 4‰,9. 8‰,12. 2 ‰） of the peripheral blood cells of C. carpio in the control,G1,G2 and G3 were positively correlated with the concentration of Cu2+after C. carpio were exposed to Cu2+for 48 hrs. Under the stress of Cu2+for 48 h the tail DNA content（ 2. 91%,5. 74%,8. 43%,11. 28%）,tail length（ 47,74,108,112 μm） and Olive tail moment（ 4. 51,9. 39,17. 68,18. 62） in the control,G1,G2 and G3 all tended to increase with the rise of Cu2+concentration and they were significantly higher in the groups G2 and G3 than in the control group（ P ＜ 0. 05）. The results of either the micronucleus test or the comet assay corroborated the genetic damage by Cu2+to the peripheral blood cells of C. carpio from different aspects. In particular,the micronucleus test was high in sensitivity,low in cost,and simple in steps and will have a high potential of application and extension in the test of ecogenotoxicity.
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DNA Damage of Cu2+ to Cyprinus carpio

doi: 10.15886/j.cnki.rdswxb.2018.04.004
  • 1. College of Materials and Chemical Engineering, Hainan University, Haikou, Hainan 570228, China;
  • 2. State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University), Haikou, Hainan 570228, China
  • Received Date: 2018-08-24
  • Rev Recd Date: 2018-10-25

Abstract: Common carp（ Cyprinus carpio） was cultured and exposed to different concentrations of Cu2+,and the DNA damage of the peripheral blood cells of C. carpio was analyzed by using the micronucleus test and the comet assay. The results showed that the rates of comet（ 17. 7%,44. 4%,60. 0%,88. 9%） and micronucleus（ 0. 4‰,8. 4‰,9. 8‰,12. 2 ‰） of the peripheral blood cells of C. carpio in the control,G1,G2 and G3 were positively correlated with the concentration of Cu2+after C. carpio were exposed to Cu2+for 48 hrs. Under the stress of Cu2+for 48 h the tail DNA content（ 2. 91%,5. 74%,8. 43%,11. 28%）,tail length（ 47,74,108,112 μm） and Olive tail moment（ 4. 51,9. 39,17. 68,18. 62） in the control,G1,G2 and G3 all tended to increase with the rise of Cu2+concentration and they were significantly higher in the groups G2 and G3 than in the control group（ P ＜ 0. 05）. The results of either the micronucleus test or the comet assay corroborated the genetic damage by Cu2+to the peripheral blood cells of C. carpio from different aspects. In particular,the micronucleus test was high in sensitivity,low in cost,and simple in steps and will have a high potential of application and extension in the test of ecogenotoxicity.

LIU Qiuyu, CHEN Xin, QIN Zongmin, ZHUO Wen, TANG Min. DNA Damage of Cu2+ to Cyprinus carpio[J]. Journal of Tropical Biology, 2018, 9(4): 388-392. doi: 10.15886/j.cnki.rdswxb.2018.04.004
Citation: LIU Qiuyu, CHEN Xin, QIN Zongmin, ZHUO Wen, TANG Min. DNA Damage of Cu2+ to Cyprinus carpio[J]. Journal of Tropical Biology, 2018, 9(4): 388-392. doi: 10.15886/j.cnki.rdswxb.2018.04.004
shu

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