Tissue Culture and Rapid Propagation of Cerasus serrulata var. lannesiana ’Sirotae’

Key words: 
• ^{Cerasus serrulata} var.^lannesiana ’Sirotae’ /
• tissue culture /
• rapid propagation /
• phytohormone

Abstract: The stem of Cerasus serrulata var. lannesiana ‘Sirotae’with buds were used as explants,sterilized and cultured on mediums with various combinations and concentrations of plant growth regulators for rapid progagation. The explants were cultured in a medium of MS + 6-BA 1. 0 mg·L^-1+ NAA 0. 1 mg·L^-1 to induce shoots. The shoots were then subcultured in the medium of MS + 6-BA 1. 0 mg·L^-1+ NAA 0. 1 mg·L^-1+GA35. 0 mg·L^-1 and then transferred onto the medium of MS + 6-BA 1. 0 mg/L + 2,4-D 0. 2 mg·L^-1 for proliferation. The proliferation coefficient was 4. 2 under these mediums. The plantlets were cultured on a rooting medium of 1/2 MS + NAA 0. 5 mg·L^-1,and the rooting rate was 93. 63%. The rooted plantlets were transplanted in the substrate which was composed of pearlite,peat and vermiculite at a ratio of 1∶ 1∶ 1,and their survival rate was up to 80% after they hardened off outdoor for 30 d.