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Volume 9 Issue 2
Apr.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(2): 170-175

Tang Zhijuan, Zhu Shousong, Yu Xinyi, Zhao Rui, Niu Xiaolei, Chen Yinhua, Geng Mengting. Cloning and Expression Analysis of MeRbohE Gene in Cassava[J]. Journal of Tropical Biology, 2018, 9(2): 170-175. doi: 10.15886/j.cnki.rdswxb.2018.02.007
Citation: Tang Zhijuan, Zhu Shousong, Yu Xinyi, Zhao Rui, Niu Xiaolei, Chen Yinhua, Geng Mengting. Cloning and Expression Analysis of MeRbohE Gene in Cassava[J]. Journal of Tropical Biology, 2018, 9(2): 170-175. doi: 10.15886/j.cnki.rdswxb.2018.02.007
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Cloning and Expression Analysis of MeRbohE Gene in Cassava

doi: 10.15886/j.cnki.rdswxb.2018.02.007

  • Institute of Tropical Agriculture and Forestry of Hainan University, Hainan University, Hainan Haikou 570228/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Hainan Haikou 570228 China

  • Received Date: 2018-03-20
  • Rev Recd Date: 2018-04-15
  • In this paper, we cloned a Rboh gene from cassava-MeRbohE, the whole length of the gene is 5 397 bp,open reading frame is 2 775 bp, encoding 925 amino acids, the molecular weight of the protein is 104.8 KDa, and the theoretical isoelectric point of the protein is 8.90, the protein structure has typical plant Rboh gene family characteristics. The results of q RT-PCR showed that the expression of MeRbohE gene was the highest in the roots,followed by the stems, and the lowest in the leaves; the expression of MeRbohE gene was up-regulated after being induced by low temperature（4 ℃）, high salt（300 mmol·L-1 Na Cl） and 100 mmol·L-1 ABA. The results indicated that the gene could respond to biological stress and lay a foundation for elucidating the mechanism of cassava Rboh gene in stress resistance.
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Cloning and Expression Analysis of MeRbohE Gene in Cassava

doi: 10.15886/j.cnki.rdswxb.2018.02.007
  • Institute of Tropical Agriculture and Forestry of Hainan University, Hainan University, Hainan Haikou 570228/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Hainan Haikou 570228 China
  • Received Date: 2018-03-20
  • Rev Recd Date: 2018-04-15

Abstract: In this paper, we cloned a Rboh gene from cassava-MeRbohE, the whole length of the gene is 5 397 bp,open reading frame is 2 775 bp, encoding 925 amino acids, the molecular weight of the protein is 104.8 KDa, and the theoretical isoelectric point of the protein is 8.90, the protein structure has typical plant Rboh gene family characteristics. The results of q RT-PCR showed that the expression of MeRbohE gene was the highest in the roots,followed by the stems, and the lowest in the leaves; the expression of MeRbohE gene was up-regulated after being induced by low temperature（4 ℃）, high salt（300 mmol·L-1 Na Cl） and 100 mmol·L-1 ABA. The results indicated that the gene could respond to biological stress and lay a foundation for elucidating the mechanism of cassava Rboh gene in stress resistance.

Tang Zhijuan, Zhu Shousong, Yu Xinyi, Zhao Rui, Niu Xiaolei, Chen Yinhua, Geng Mengting. Cloning and Expression Analysis of MeRbohE Gene in Cassava[J]. Journal of Tropical Biology, 2018, 9(2): 170-175. doi: 10.15886/j.cnki.rdswxb.2018.02.007
Citation: Tang Zhijuan, Zhu Shousong, Yu Xinyi, Zhao Rui, Niu Xiaolei, Chen Yinhua, Geng Mengting. Cloning and Expression Analysis of MeRbohE Gene in Cassava[J]. Journal of Tropical Biology, 2018, 9(2): 170-175. doi: 10.15886/j.cnki.rdswxb.2018.02.007
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