Physical Location of 5 Members of MYC Gene Family on the Chromosomes in Hevea brasiliensis

Key words: 
• Hevea brasiliensis /
• MYC gene family /
• fluorescence in situ hybridization（FISH） /
• physical location

Abstract: MYC transcription factors or Myelocytomatosis proteins（MYCs） play a very important role in the signal transduction of jasmonic acid. Five genes of the MYC gene family of Hevea brasiliensis had been cloned, and their functions had been reported. However, the specific locations of the MYC gene family members on the chromosome have not been reported. In this experiment, the locations of 5 genes of MYC family of H. brasiliensis were physically located on the chromosomes by using fluorescence in situ hybridization（FISH） technique. Rubber clone Reyan 7-33-97 of H. brasiliensis was used as an experimental material. The results show that both HblMYC2 and HblMYC5 genes were located on the long arm of chromosome 9, with an average distance of 47.96 and 47.22 from the centromere, respectively; HblMYC1 and HblMYC3 genes were located on the long arm of 6 th and 8 th chromosomes, respectively, with a respective average distance of 32.56 and 62.77 from the centromere; HblMYC4 was located on the short arm of 5 th chromosome, with an average distance of 55.67 from the centromere. This study explored the specific locations of the 5 genes and their relationships on chromosomes, which can provide cytological basis for genomics and molecular marker assisted breeding of H. brasiliensis.