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Volume 9 Issue 2
Apr.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(2): 136-141

ZENG Jifeng, PU Wenyuan, GUO Guiying, YANG Nuo, HOU Guofeng, ZHENG Jiping. Isolation and Identification of Pathogenic Aeromonas hydrophila spp. hydrophila from Crocodylus siamensis[J]. Journal of Tropical Biology, 2018, 9(2): 136-141. doi: 10.15886/j.cnki.rdswxb.2018.02.002
Citation: ZENG Jifeng, PU Wenyuan, GUO Guiying, YANG Nuo, HOU Guofeng, ZHENG Jiping. Isolation and Identification of Pathogenic Aeromonas hydrophila spp. hydrophila from Crocodylus siamensis[J]. Journal of Tropical Biology, 2018, 9(2): 136-141. doi: 10.15886/j.cnki.rdswxb.2018.02.002
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Isolation and Identification of Pathogenic Aeromonas hydrophila spp. hydrophila from Crocodylus siamensis

doi: 10.15886/j.cnki.rdswxb.2018.02.002

  • Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2017-07-24
  • Rev Recd Date: 2017-11-20
  • A dominant bacterial strain isolated from the lesion tissues of the inspected dead Crocodylus siamensis in Lingshui,Hainan Province was identified via morphological observation,and physiological and biochemical test.The results showed that this isolated strain was gram-negative and was almost identical in characteristics to Aeromonas hydrophila. The phylogenetic analysis based on 16 S rRNA gene sequences further indicated that the isolate was clustered in the genus Aeromonas,with its sequence having the maximum similarity（99. 9%） with that of Aeromonas hydrophila subsp. hydrophila. The artificial infection test showed that the isolate was highly morbigenous to NIH mice with the half median lethal dose of 2. 5 × 105 cfu·m L-1. The sensitivity tests of this isolate with 27 antibiotics demonstrated that the isolate was sensitive to 13 antibiotics,such as amikacin,ciprofloxacin,cefoperazone,etc,and resistant to 9 antibiotics such as erythromycin,ampicillin,streptomycin etc.
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Isolation and Identification of Pathogenic Aeromonas hydrophila spp. hydrophila from Crocodylus siamensis

doi: 10.15886/j.cnki.rdswxb.2018.02.002
  • Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2017-07-24
  • Rev Recd Date: 2017-11-20

Abstract: A dominant bacterial strain isolated from the lesion tissues of the inspected dead Crocodylus siamensis in Lingshui,Hainan Province was identified via morphological observation,and physiological and biochemical test.The results showed that this isolated strain was gram-negative and was almost identical in characteristics to Aeromonas hydrophila. The phylogenetic analysis based on 16 S rRNA gene sequences further indicated that the isolate was clustered in the genus Aeromonas,with its sequence having the maximum similarity（99. 9%） with that of Aeromonas hydrophila subsp. hydrophila. The artificial infection test showed that the isolate was highly morbigenous to NIH mice with the half median lethal dose of 2. 5 × 105 cfu·m L-1. The sensitivity tests of this isolate with 27 antibiotics demonstrated that the isolate was sensitive to 13 antibiotics,such as amikacin,ciprofloxacin,cefoperazone,etc,and resistant to 9 antibiotics such as erythromycin,ampicillin,streptomycin etc.

ZENG Jifeng, PU Wenyuan, GUO Guiying, YANG Nuo, HOU Guofeng, ZHENG Jiping. Isolation and Identification of Pathogenic Aeromonas hydrophila spp. hydrophila from Crocodylus siamensis[J]. Journal of Tropical Biology, 2018, 9(2): 136-141. doi: 10.15886/j.cnki.rdswxb.2018.02.002
Citation: ZENG Jifeng, PU Wenyuan, GUO Guiying, YANG Nuo, HOU Guofeng, ZHENG Jiping. Isolation and Identification of Pathogenic Aeromonas hydrophila spp. hydrophila from Crocodylus siamensis[J]. Journal of Tropical Biology, 2018, 9(2): 136-141. doi: 10.15886/j.cnki.rdswxb.2018.02.002
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