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Volume 9 Issue 1
Jan.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(1): 12-20

LIN Xiangui, ZHAI Jinling, HUANG Xi. Cloning and Expression Profiling of Transcription Factors HbMYBs of Hevea brasiliensis[J]. Journal of Tropical Biology, 2018, 9(1): 12-20. doi: 10.15886/j.cnki.rdswxb.2018.01.002
Citation: LIN Xiangui, ZHAI Jinling, HUANG Xi. Cloning and Expression Profiling of Transcription Factors HbMYBs of Hevea brasiliensis[J]. Journal of Tropical Biology, 2018, 9(1): 12-20. doi: 10.15886/j.cnki.rdswxb.2018.01.002
shu

Cloning and Expression Profiling of Transcription Factors HbMYBs of Hevea brasiliensis

doi: 10.15886/j.cnki.rdswxb.2018.01.002

  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China

  • Received Date: 2017-10-09
  • Rev Recd Date: 2017-11-21
  • Hevea brasiliensis is the main source of natural rubber.MYB gene family plays an important role in plant growth,development and responses under environmental stresses.Through homologous sequence alignment,five HbMYBs related genes were cloned in H.brasiliensis and termed as HbMYB15,HbMYB21,HbMYB24,HbMYB35 and HbMYB75 according to the similarity with the homologous gene in Arabidopsis.The ORF（open reading frame） lengths of these five HbMYBs are 651 bp,984 bp,999 bp,1 095 bp and 933 bp,respectively.Bioinformatics analysis showed that all the HbMYBs protein contain MYB conserved domains and belong to R2R3 type MYB transcription factor.Yeast self-activation experiments showed that the five HbMYBs proteins had transcriptional activity,indicating that the five HbMYBs genes are potential transcription factors.The results of quantitative RT-PCR showed that the five HbMYBs genes were regulated by treatment of wounding,ethylene or jasmonic acid,indicating that these genes may be involved in the plant defense response related to ethylene and jasmonic acid signaling pathway.
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Cloning and Expression Profiling of Transcription Factors HbMYBs of Hevea brasiliensis

doi: 10.15886/j.cnki.rdswxb.2018.01.002
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
  • Received Date: 2017-10-09
  • Rev Recd Date: 2017-11-21

Abstract: Hevea brasiliensis is the main source of natural rubber.MYB gene family plays an important role in plant growth,development and responses under environmental stresses.Through homologous sequence alignment,five HbMYBs related genes were cloned in H.brasiliensis and termed as HbMYB15,HbMYB21,HbMYB24,HbMYB35 and HbMYB75 according to the similarity with the homologous gene in Arabidopsis.The ORF（open reading frame） lengths of these five HbMYBs are 651 bp,984 bp,999 bp,1 095 bp and 933 bp,respectively.Bioinformatics analysis showed that all the HbMYBs protein contain MYB conserved domains and belong to R2R3 type MYB transcription factor.Yeast self-activation experiments showed that the five HbMYBs proteins had transcriptional activity,indicating that the five HbMYBs genes are potential transcription factors.The results of quantitative RT-PCR showed that the five HbMYBs genes were regulated by treatment of wounding,ethylene or jasmonic acid,indicating that these genes may be involved in the plant defense response related to ethylene and jasmonic acid signaling pathway.

LIN Xiangui, ZHAI Jinling, HUANG Xi. Cloning and Expression Profiling of Transcription Factors HbMYBs of Hevea brasiliensis[J]. Journal of Tropical Biology, 2018, 9(1): 12-20. doi: 10.15886/j.cnki.rdswxb.2018.01.002
Citation: LIN Xiangui, ZHAI Jinling, HUANG Xi. Cloning and Expression Profiling of Transcription Factors HbMYBs of Hevea brasiliensis[J]. Journal of Tropical Biology, 2018, 9(1): 12-20. doi: 10.15886/j.cnki.rdswxb.2018.01.002
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