Expression Analysis of 25 WRKY Transcription Factors in Cassava under Biotic Stresses
doi: 10.15886/j.cnki.rdswxb.2017.01.003
- Received Date: 2016-12-07
- Rev Recd Date: 2017-01-29
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Key words:
- Cassava /
- WRKY transcriptional factor /
- expression pattern
Abstract: WRKY family is one of the largest families of transcription factors in higher plants. The WRKY transcription factors play a central role in signal networks and plant growth. The WRKY transcription factors have been extensively studied in response to biotic stresses in model plants,but little information about their roles in cassava is available. According to the WRKY sequences of Arabidopsis and rice,and cassava genome sequences,25 WRKY genes in cassava were predicted and analyzed including their phylogenetic relationships and conserved domains. Expression profiles of these genes in response to pathogen were analyzed by quantitative PCR. The results showed that the 25 identified Me WRKY genes in cassava had the conserved WRKY domain and zinc-finger structure and had distinct expression patterns in response to pathogen infection,out of which 16 were significantly up-or down-regulated,implying that these members might participate in defense response to biotic stresses in cassava.
Citation: | LI Ke, XIONG Xi, XIAO Xiaorong, LI Yumin, NIU Xiaolei, LI Chunxia, CHEN Yinhua. Expression Analysis of 25 WRKY Transcription Factors in Cassava under Biotic Stresses[J]. Journal of Tropical Biology, 2017, 8(1): 14-21,28. doi: 10.15886/j.cnki.rdswxb.2017.01.003 |