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Volume 7 Issue 4
Dec.  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(4): 497-499

YE Linxiong, FU Qingliu, HU Rong, LI Jiahui. Extracting of Genomic DNA from Mealybugs（Hemiptera: Pseudococcidae） Without Conferring External Morphological Damage[J]. Journal of Tropical Biology, 2016, 7(4): 497-499. doi: 10.15886/j.cnki.rdswxb.2016.04.015
Citation: YE Linxiong, FU Qingliu, HU Rong, LI Jiahui. Extracting of Genomic DNA from Mealybugs（Hemiptera: Pseudococcidae） Without Conferring External Morphological Damage[J]. Journal of Tropical Biology, 2016, 7(4): 497-499. doi: 10.15886/j.cnki.rdswxb.2016.04.015
shu

Extracting of Genomic DNA from Mealybugs（Hemiptera: Pseudococcidae） Without Conferring External Morphological Damage

doi: 10.15886/j.cnki.rdswxb.2016.04.015

  • College of Environment and Plant Protection, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2016-07-13
  • Mealybug Ferrisia virgata Cockerell was digested with Proteinase K solution rather than 10% KOH to extract its genomic DNA. F. virgata was pretreated before extraction by grinding the whole mealybug,pricking the mealybug with micro-pins on abdomen or without any treatment to the mealybug. Genomic DNA was extracted by the digestion kit following its directions,and the rest of the mealybug left after digestion was used for slide preparation. The results show genomic DNA was extracted from the mealybug with all the three pre-treatments and that the rest of the mealybug left in the latter two pretreatments was good to make slides. This is a new protocol for genomic DNA extraction combined with permanent slides mounting in mealybugs. It would be useful for DNA barcoding and systematic research of mealybugs.
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Extracting of Genomic DNA from Mealybugs（Hemiptera: Pseudococcidae） Without Conferring External Morphological Damage

doi: 10.15886/j.cnki.rdswxb.2016.04.015
  • College of Environment and Plant Protection, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2016-07-13

Abstract: Mealybug Ferrisia virgata Cockerell was digested with Proteinase K solution rather than 10% KOH to extract its genomic DNA. F. virgata was pretreated before extraction by grinding the whole mealybug,pricking the mealybug with micro-pins on abdomen or without any treatment to the mealybug. Genomic DNA was extracted by the digestion kit following its directions,and the rest of the mealybug left after digestion was used for slide preparation. The results show genomic DNA was extracted from the mealybug with all the three pre-treatments and that the rest of the mealybug left in the latter two pretreatments was good to make slides. This is a new protocol for genomic DNA extraction combined with permanent slides mounting in mealybugs. It would be useful for DNA barcoding and systematic research of mealybugs.

YE Linxiong, FU Qingliu, HU Rong, LI Jiahui. Extracting of Genomic DNA from Mealybugs（Hemiptera: Pseudococcidae） Without Conferring External Morphological Damage[J]. Journal of Tropical Biology, 2016, 7(4): 497-499. doi: 10.15886/j.cnki.rdswxb.2016.04.015
Citation: YE Linxiong, FU Qingliu, HU Rong, LI Jiahui. Extracting of Genomic DNA from Mealybugs（Hemiptera: Pseudococcidae） Without Conferring External Morphological Damage[J]. Journal of Tropical Biology, 2016, 7(4): 497-499. doi: 10.15886/j.cnki.rdswxb.2016.04.015
shu

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