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Volume 7 Issue 4
Dec.  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(4): 427-434

LIAO HuaLan, LI XiuQiong, LI Ke, LI BoLing, XIONG XI, SU Tong, LI ChunXia, CHEN YinHua, LUO LiJun. The Optimization System for Agrobacterium-mediated Genetic Transformation of Cassava[J]. Journal of Tropical Biology, 2016, 7(4): 427-434. doi: 10.15886/j.cnki.rdswxb.2016.04.004
Citation: LIAO HuaLan, LI XiuQiong, LI Ke, LI BoLing, XIONG XI, SU Tong, LI ChunXia, CHEN YinHua, LUO LiJun. The Optimization System for Agrobacterium-mediated Genetic Transformation of Cassava[J]. Journal of Tropical Biology, 2016, 7(4): 427-434. doi: 10.15886/j.cnki.rdswxb.2016.04.004
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The Optimization System for Agrobacterium-mediated Genetic Transformation of Cassava

doi: 10.15886/j.cnki.rdswxb.2016.04.004

  • The Agricultural College of Hainan University/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2016-03-16
  • Cassava axillary buds was used as explants,by adding different concentrations of 2,4-D and picloram to explore the best hormone condition for inducing somatic embryos and FEC; Using optimized regeneration system,studying the infection concentration、infection time of of Agrobacterium and the Screening concentration of hygromycin. The results show that: 12 mg·L-1 picloram is the best hormone concentration to induction somatic embryo and FEC; the optimal conditions for the conversion efficiency of FEC and the plant regeneration is OD600= 0. 4 of Agrobacterium and 30 min infection time,By increasing the concentration of hygromycin( 0,5,8,15 mg·L-1) selection is more advantageous to plant regeneration. The results of the study have optimized the existing cassava genetic transformation system,which provides a platform for cassava genetic engineering breeding.
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The Optimization System for Agrobacterium-mediated Genetic Transformation of Cassava

doi: 10.15886/j.cnki.rdswxb.2016.04.004
  • The Agricultural College of Hainan University/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2016-03-16

Abstract: Cassava axillary buds was used as explants,by adding different concentrations of 2,4-D and picloram to explore the best hormone condition for inducing somatic embryos and FEC; Using optimized regeneration system,studying the infection concentration、infection time of of Agrobacterium and the Screening concentration of hygromycin. The results show that: 12 mg·L-1 picloram is the best hormone concentration to induction somatic embryo and FEC; the optimal conditions for the conversion efficiency of FEC and the plant regeneration is OD600= 0. 4 of Agrobacterium and 30 min infection time,By increasing the concentration of hygromycin( 0,5,8,15 mg·L-1) selection is more advantageous to plant regeneration. The results of the study have optimized the existing cassava genetic transformation system,which provides a platform for cassava genetic engineering breeding.

LIAO HuaLan, LI XiuQiong, LI Ke, LI BoLing, XIONG XI, SU Tong, LI ChunXia, CHEN YinHua, LUO LiJun. The Optimization System for Agrobacterium-mediated Genetic Transformation of Cassava[J]. Journal of Tropical Biology, 2016, 7(4): 427-434. doi: 10.15886/j.cnki.rdswxb.2016.04.004
Citation: LIAO HuaLan, LI XiuQiong, LI Ke, LI BoLing, XIONG XI, SU Tong, LI ChunXia, CHEN YinHua, LUO LiJun. The Optimization System for Agrobacterium-mediated Genetic Transformation of Cassava[J]. Journal of Tropical Biology, 2016, 7(4): 427-434. doi: 10.15886/j.cnki.rdswxb.2016.04.004
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