AFLP Analysis of Genetic Diversity in Five Selected Lines of abalone Haliotis diversicolor supertexta

Key words: 
• Haliotis diversicolor supertexta /
• selected line /
• AFLP /
• genetic diversity

Abstract: AFLP technique was used to analyze the genetic diversity of the fifth generation of cultured stocks of abalone Haliotis diversicolor supertexta. A total of 258 reproducible bands were amplified with 5 pairs of AFLP primers from 150 individuals of the 5 selected lines of the fifth generation of the cultured stocks（ 30 individuals each selected line）,of which 184 bands were polymorphic,accounting for 71. 32%. For all the selected lines the percentage polymorphic loci and average heterozygosity were 78. 43%-97. 06% and 0. 2143-0. 2653,respectively; and the Nei’s genetic identity and genetic distance among the selected lines were 0. 990 8-0. 998 6and 0. 001 4-0. 009 2,respectively. AMOVA analysis showed a large proportion（ 60. 7%） of genetic variation resided within the selected lines,while only a small proportion（ 39. 3%） of genetic variation resided among the selected lines. The pairwise φ_ST values ranged from 0. 030 6 to 0. 039 2 for the selected lines,and no significant genetic differentiation was detected among the selected lines of the cultured stocks of the abalone H. diversicolor supertexta（ P ＞ 0. 001）.