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Volume 7 Issue 4
Dec.  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(4): 409-416

XIA Liqun, WANG Mei, LAI Jiebin, CHEN Ruimin, XU Liang, LU Yishan. Establishment of a Zebrafish Model for Nocardia seriolae and Histopathological Study[J]. Journal of Tropical Biology, 2016, 7(4): 409-416. doi: 10.15886/j.cnki.rdswxb.2016.04.001
Citation: XIA Liqun, WANG Mei, LAI Jiebin, CHEN Ruimin, XU Liang, LU Yishan. Establishment of a Zebrafish Model for Nocardia seriolae and Histopathological Study[J]. Journal of Tropical Biology, 2016, 7(4): 409-416. doi: 10.15886/j.cnki.rdswxb.2016.04.001
shu

Establishment of a Zebrafish Model for Nocardia seriolae and Histopathological Study

doi: 10.15886/j.cnki.rdswxb.2016.04.001

  • 1. College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China;

  • 2. Guangdong Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, Guangdong 524088, China;

  • 3. Guangdong Higher Education Institutions Key Laboratory for for Controlling of Diseases of Aquatic Economic Animals,, Zhanjiang, Guangdong 524088, China

  • Received Date: 2016-10-28
  • Fish nocardiosis has been spread in the south of China in recent years and has a huge adverse effect on the fishery industry. Nocardia seriolae is a leading cause of fish nocardiosis and has been reported in about 20 fish species. Zebrafish（ Danio rerio） were infected with a strong virulent? strain of N. seriolae ZJ0503 at different concentrations through intraperitoneal injection. A zebrafish model for N. seriolae was established through the observation of clinical symptoms,pathologic autopsy,pathogen isolation,50% lethal dose（ LD50） analysis and histopathological studies. The results show that an obvious infection happened in the zebrafish after challenged with N. seriolae ZJ0503. The LD50 of N. seriolae ZJ0503 was 3. 39 × 108 CFU/fish. Obvious pathological changes were observed in the zebrafish tissues,such as kidney,liver,pancreas,gill,skin and muscle. This study provides evidence that the zebrafish can be used as a model for N. seriolae infection,and the model is useful to study the pathogenic mechanism of fish nocardiosis for development of N. seriolae vaccine.
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Establishment of a Zebrafish Model for Nocardia seriolae and Histopathological Study

doi: 10.15886/j.cnki.rdswxb.2016.04.001
  • 1. College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China;
  • 2. Guangdong Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, Guangdong 524088, China;
  • 3. Guangdong Higher Education Institutions Key Laboratory for for Controlling of Diseases of Aquatic Economic Animals,, Zhanjiang, Guangdong 524088, China
  • Received Date: 2016-10-28

Abstract: Fish nocardiosis has been spread in the south of China in recent years and has a huge adverse effect on the fishery industry. Nocardia seriolae is a leading cause of fish nocardiosis and has been reported in about 20 fish species. Zebrafish（ Danio rerio） were infected with a strong virulent? strain of N. seriolae ZJ0503 at different concentrations through intraperitoneal injection. A zebrafish model for N. seriolae was established through the observation of clinical symptoms,pathologic autopsy,pathogen isolation,50% lethal dose（ LD50） analysis and histopathological studies. The results show that an obvious infection happened in the zebrafish after challenged with N. seriolae ZJ0503. The LD50 of N. seriolae ZJ0503 was 3. 39 × 108 CFU/fish. Obvious pathological changes were observed in the zebrafish tissues,such as kidney,liver,pancreas,gill,skin and muscle. This study provides evidence that the zebrafish can be used as a model for N. seriolae infection,and the model is useful to study the pathogenic mechanism of fish nocardiosis for development of N. seriolae vaccine.

XIA Liqun, WANG Mei, LAI Jiebin, CHEN Ruimin, XU Liang, LU Yishan. Establishment of a Zebrafish Model for Nocardia seriolae and Histopathological Study[J]. Journal of Tropical Biology, 2016, 7(4): 409-416. doi: 10.15886/j.cnki.rdswxb.2016.04.001
Citation: XIA Liqun, WANG Mei, LAI Jiebin, CHEN Ruimin, XU Liang, LU Yishan. Establishment of a Zebrafish Model for Nocardia seriolae and Histopathological Study[J]. Journal of Tropical Biology, 2016, 7(4): 409-416. doi: 10.15886/j.cnki.rdswxb.2016.04.001
shu

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