Optimization of Fermentation Conditions for Production of Arginine Deiminase from Pseudomonas alcaligenes

Key words: 
• Pseudomonas alcaligenes /
• arginine deiminase /
• fermentation /
• activity

Abstract: Pseudomonas alcaligenes was cultured on various media to optimize fermentation conditions to produce arginine deiminase（ ADI）. The fermentation conditions for P. alcaligenes to produce ADI were found optimum when 10g·L^-1 sucrose was used as the carbon source,6 g·L^-1 peptone as the optimal nitrogen source,10 g·L^-1 arginineas the inducer,inoculation amount was 4%,liquid volume was 30 m L per 100 m L,incubation temperature was 29 ℃,initial p H was 8. 5,shaker rotation speed was 150 r·min^-1 and fermentation time was 24 h.Under these fermentation conditions the arginine deiminase produced from P. alcaligenes had the highest activities.