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Volume 6 Issue 1
Jan.  2015
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GAO Yunkai, ZHANG Rongyi, ZHONG Liwen, SONG Haichao, SHI Xuequn. Classification and Identification of a New Isolated Yeast Strain and Its Biocontrol Activity against Postharvest Anthracnose of Mango Fruit[J]. Journal of Tropical Biology, 2015, 6(1): 47-52. doi: 10.15886/j.cnki.rdswxb.2015.01.007
Citation: GAO Yunkai, ZHANG Rongyi, ZHONG Liwen, SONG Haichao, SHI Xuequn. Classification and Identification of a New Isolated Yeast Strain and Its Biocontrol Activity against Postharvest Anthracnose of Mango Fruit[J]. Journal of Tropical Biology, 2015, 6(1): 47-52. doi: 10.15886/j.cnki.rdswxb.2015.01.007

Classification and Identification of a New Isolated Yeast Strain and Its Biocontrol Activity against Postharvest Anthracnose of Mango Fruit

doi: 10.15886/j.cnki.rdswxb.2015.01.007
  • Received Date: 2014-03-03
  • A new antagonistic yeast strain( LZ5) was isolated from a mango orchard in Hainan Province. Antagonistic activity of this yeast strain was evaluated against postharvest anthracnose of mango fruit caused by Colletotrichum gloeosporioides through in vitro and in vivo assays. In vivo inoculation assay revealed that 1 × 108cfu·m L-1 of the yeast strain had significant biocontrol activity against C. gloeosporioides. The lesion diameter of the anthracnose pathogenic fungi was 5. 80 mm in the control and 0. 33 mm in the test group after 4 days of inoculation at 25°C,and 26. 3 mm in the control and 17. 6 mm in the test group after 6 days at 25°C,respectively,and the disease incidence was 100% in the control and 20% in the test group after 4 days of inoculation. Moreover,it was observed that the yeast strain LZ5 grew rapidly on the wounds of mango fruit. The yeast cell population increased 62. 6-fold 24 h after inoculation and was then stabilized for the rest of the storage period. The yeast strain LZ5 was identified as Meyerozyma guilliermondii.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Classification and Identification of a New Isolated Yeast Strain and Its Biocontrol Activity against Postharvest Anthracnose of Mango Fruit

doi: 10.15886/j.cnki.rdswxb.2015.01.007

Abstract: A new antagonistic yeast strain( LZ5) was isolated from a mango orchard in Hainan Province. Antagonistic activity of this yeast strain was evaluated against postharvest anthracnose of mango fruit caused by Colletotrichum gloeosporioides through in vitro and in vivo assays. In vivo inoculation assay revealed that 1 × 108cfu·m L-1 of the yeast strain had significant biocontrol activity against C. gloeosporioides. The lesion diameter of the anthracnose pathogenic fungi was 5. 80 mm in the control and 0. 33 mm in the test group after 4 days of inoculation at 25°C,and 26. 3 mm in the control and 17. 6 mm in the test group after 6 days at 25°C,respectively,and the disease incidence was 100% in the control and 20% in the test group after 4 days of inoculation. Moreover,it was observed that the yeast strain LZ5 grew rapidly on the wounds of mango fruit. The yeast cell population increased 62. 6-fold 24 h after inoculation and was then stabilized for the rest of the storage period. The yeast strain LZ5 was identified as Meyerozyma guilliermondii.

GAO Yunkai, ZHANG Rongyi, ZHONG Liwen, SONG Haichao, SHI Xuequn. Classification and Identification of a New Isolated Yeast Strain and Its Biocontrol Activity against Postharvest Anthracnose of Mango Fruit[J]. Journal of Tropical Biology, 2015, 6(1): 47-52. doi: 10.15886/j.cnki.rdswxb.2015.01.007
Citation: GAO Yunkai, ZHANG Rongyi, ZHONG Liwen, SONG Haichao, SHI Xuequn. Classification and Identification of a New Isolated Yeast Strain and Its Biocontrol Activity against Postharvest Anthracnose of Mango Fruit[J]. Journal of Tropical Biology, 2015, 6(1): 47-52. doi: 10.15886/j.cnki.rdswxb.2015.01.007

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