DNA Extraction and SRAP Clustering Analysis of Morinda citrifolia

Key words: 
• Morinda citrifolia /
• DNA extraction /
• SRAP molecular marker /
• cluster analysis

Abstract: Morinda citrifolia is a famous tropical medicinal plant. According to the retrieval,few reports are found about M. citrifolia germplasm resources. The genetic diversity of M. citrifolia was analyzed by using the SRAP molecular marker technology. The DNA of M. citrifolia was extracted by using the improved extraction method,and high purity of DNA was extracted. Seventy eight accessions of Morinda citrifolia germplasm were amplified by SRAP markers with 12 pairs of primers,and a total of 11 154 polymorphic bands were produced,of which 3 792 bands or 33. 99% are polymorphic,indicating good polymorphism among the germplasm. SRAP molecular marker clustering showed that 78 accessions of M. citrifolia germplasm were clustered into 2 groups when the genetic distance was 0. 66,namely,Hainan local and Paracel Islands as 1 group and the rest of the germplasm as the other group. The 78 accessions of M. citrifolia germplasm were clustered into 6 groups when the genetic distance was 0. 86 as the threshold,from the top to the bottom: Wanwei 2,Singapore,Tahitian,Wanwei 1,Hainan native,Paracel Islands. The clustering results showed a high similarity between Tahiti and Wanwei 1,which indicated that Tahiti had a higher genetic relationship with Wanwei 1,followed by the genetic relationship between Singapore and Wanwei 2.