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Volume 5 Issue 4
Dec.  2014
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Article Navigation >  Journal of Tropical Biology  > 2014  >  5(4): 312-319,356

ZHAO Guangjun, ZHOU Yongcan, YANG Hui, CAI Yan, ZHANG Han, XIE Zhenyu, WANG Shifeng, LI Cong. Isolation,Identification and Diversity Analysis of Antagonistic Bacteria Against Tilapia Pathogen Streptococcus agalactiae[J]. Journal of Tropical Biology, 2014, 5(4): 312-319,356. doi: 10.15886/j.cnki.rdswxb.2014.04.002
Citation: ZHAO Guangjun, ZHOU Yongcan, YANG Hui, CAI Yan, ZHANG Han, XIE Zhenyu, WANG Shifeng, LI Cong. Isolation,Identification and Diversity Analysis of Antagonistic Bacteria Against Tilapia Pathogen Streptococcus agalactiae[J]. Journal of Tropical Biology, 2014, 5(4): 312-319,356. doi: 10.15886/j.cnki.rdswxb.2014.04.002
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Isolation,Identification and Diversity Analysis of Antagonistic Bacteria Against Tilapia Pathogen Streptococcus agalactiae

doi: 10.15886/j.cnki.rdswxb.2014.04.002

  • 1. State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Bioresources, Hainan University, Haikou 570228;

  • 2. Hainan Provincial Key Laboratory for Tropical Hydrobiology & Biotechnology, College of Marine Sciences, Hainan University, Haikou 570228, China

  • Received Date: 2014-04-14
  • Antagonistic bacteria against tilapia Streptococcus agalactiae were isolated using Radial Diffusion Assay（ RDA） method and identified using 16 S r DNA sequencing technique. A phylogenetic tree was also constructed to analyze the diversity of the antagonistic bacteria isolated. Intraperitoneal injection method was used to test the toxicity of the isolated antagonistic bacteria against tilapia fishes. Results showed that 59 of the 1 759 strains of the bacteria isolated from eight freshwater ponds showed strong antagonistic effect against Streptococcus agalactiae. The diameter of the inhibition zones ranged from 10. 6 mm to 30. 8 mm with an average value of 21. 9mm.The 16 S r DNA sequencing and phytogenetic tree analysis indicated that these 59 strains of bacteria belong to 5genus and 8 species,including genus Bacillus（ 45 strains）,genus Pseudomonas（ 5 strains）,genus Enterococcus（ 2 strains）,genus Paenibacillus（ 2 stains） and genus Staphylococcus（ 5 strains）. The toxicity tolerance test showed that 22 strains were nontoxic to tilapia fishes. These nontoxic strains can be used as alternative antagonistic bacterial strains to control and prevent from tilapia Streptococcus disease in the future.
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Isolation,Identification and Diversity Analysis of Antagonistic Bacteria Against Tilapia Pathogen Streptococcus agalactiae

doi: 10.15886/j.cnki.rdswxb.2014.04.002
  • 1. State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Bioresources, Hainan University, Haikou 570228;
  • 2. Hainan Provincial Key Laboratory for Tropical Hydrobiology & Biotechnology, College of Marine Sciences, Hainan University, Haikou 570228, China
  • Received Date: 2014-04-14

Abstract: Antagonistic bacteria against tilapia Streptococcus agalactiae were isolated using Radial Diffusion Assay（ RDA） method and identified using 16 S r DNA sequencing technique. A phylogenetic tree was also constructed to analyze the diversity of the antagonistic bacteria isolated. Intraperitoneal injection method was used to test the toxicity of the isolated antagonistic bacteria against tilapia fishes. Results showed that 59 of the 1 759 strains of the bacteria isolated from eight freshwater ponds showed strong antagonistic effect against Streptococcus agalactiae. The diameter of the inhibition zones ranged from 10. 6 mm to 30. 8 mm with an average value of 21. 9mm.The 16 S r DNA sequencing and phytogenetic tree analysis indicated that these 59 strains of bacteria belong to 5genus and 8 species,including genus Bacillus（ 45 strains）,genus Pseudomonas（ 5 strains）,genus Enterococcus（ 2 strains）,genus Paenibacillus（ 2 stains） and genus Staphylococcus（ 5 strains）. The toxicity tolerance test showed that 22 strains were nontoxic to tilapia fishes. These nontoxic strains can be used as alternative antagonistic bacterial strains to control and prevent from tilapia Streptococcus disease in the future.

ZHAO Guangjun, ZHOU Yongcan, YANG Hui, CAI Yan, ZHANG Han, XIE Zhenyu, WANG Shifeng, LI Cong. Isolation,Identification and Diversity Analysis of Antagonistic Bacteria Against Tilapia Pathogen Streptococcus agalactiae[J]. Journal of Tropical Biology, 2014, 5(4): 312-319,356. doi: 10.15886/j.cnki.rdswxb.2014.04.002
Citation: ZHAO Guangjun, ZHOU Yongcan, YANG Hui, CAI Yan, ZHANG Han, XIE Zhenyu, WANG Shifeng, LI Cong. Isolation,Identification and Diversity Analysis of Antagonistic Bacteria Against Tilapia Pathogen Streptococcus agalactiae[J]. Journal of Tropical Biology, 2014, 5(4): 312-319,356. doi: 10.15886/j.cnki.rdswxb.2014.04.002
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