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Volume 5 Issue 2
Jun.  2014
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QIN Chuixin, CAO Zifeng, TAN Rirong, LIN Weibin. Detection of Acidic and Neutral Polysaccharides in Chinese Herb Concentrates[J]. Journal of Tropical Biology, 2014, 5(2): 170-173. doi: 10.15886/j.cnki.rdswxb.2014.02.004
Citation: QIN Chuixin, CAO Zifeng, TAN Rirong, LIN Weibin. Detection of Acidic and Neutral Polysaccharides in Chinese Herb Concentrates[J]. Journal of Tropical Biology, 2014, 5(2): 170-173. doi: 10.15886/j.cnki.rdswxb.2014.02.004

Detection of Acidic and Neutral Polysaccharides in Chinese Herb Concentrates

doi: 10.15886/j.cnki.rdswxb.2014.02.004
  • Received Date: 2014-05-01
  • Neutral and acidic polysaccharides were separated and prepared from Chinese herbal concentrates using the CTAB precipitation method and were then analyzed quantitatively. The results show that the monosaccharide composition of the polysaccharides in the Chinese herb concentrates are mannose,ribose,rhamnose,glucuronic acid,galactose,xylose,arabinose with molar percentages of 19. 6,1. 0,0. 9,2. 4,60. 1,7. 3,6. 1and 2. 5 respectively. Determined by using the phenol-sulfuric acid method the neutral polysaccharides varied from 54. 3% to 67. 8% of the total polysaccharides,and the acid polysaccharides did from 5. 9% 11. 8%. This detection method can be used as a reference for rapid classification of polysaccharides.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Detection of Acidic and Neutral Polysaccharides in Chinese Herb Concentrates

doi: 10.15886/j.cnki.rdswxb.2014.02.004

Abstract: Neutral and acidic polysaccharides were separated and prepared from Chinese herbal concentrates using the CTAB precipitation method and were then analyzed quantitatively. The results show that the monosaccharide composition of the polysaccharides in the Chinese herb concentrates are mannose,ribose,rhamnose,glucuronic acid,galactose,xylose,arabinose with molar percentages of 19. 6,1. 0,0. 9,2. 4,60. 1,7. 3,6. 1and 2. 5 respectively. Determined by using the phenol-sulfuric acid method the neutral polysaccharides varied from 54. 3% to 67. 8% of the total polysaccharides,and the acid polysaccharides did from 5. 9% 11. 8%. This detection method can be used as a reference for rapid classification of polysaccharides.

QIN Chuixin, CAO Zifeng, TAN Rirong, LIN Weibin. Detection of Acidic and Neutral Polysaccharides in Chinese Herb Concentrates[J]. Journal of Tropical Biology, 2014, 5(2): 170-173. doi: 10.15886/j.cnki.rdswxb.2014.02.004
Citation: QIN Chuixin, CAO Zifeng, TAN Rirong, LIN Weibin. Detection of Acidic and Neutral Polysaccharides in Chinese Herb Concentrates[J]. Journal of Tropical Biology, 2014, 5(2): 170-173. doi: 10.15886/j.cnki.rdswxb.2014.02.004

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