Advance Search

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 5 Issue 1
Mar.  2014
Turn off MathJax
Article Contents
Article Navigation >  Journal of Tropical Biology  > 2014  >  5(1): 73-77,91

YANG Xuan, LI Jun, LI Congfa, HAO Junran, XU Wentao, LIU Sixin. Two-dimensional Polyacrylamide Gel Electrophoresis Techniques for Proteomic Research of Gluconacetobacter[J]. Journal of Tropical Biology, 2014, 5(1): 73-77,91. doi: 10.15886/j.cnki.rdswxb.2014.01.003
Citation: YANG Xuan, LI Jun, LI Congfa, HAO Junran, XU Wentao, LIU Sixin. Two-dimensional Polyacrylamide Gel Electrophoresis Techniques for Proteomic Research of Gluconacetobacter[J]. Journal of Tropical Biology, 2014, 5(1): 73-77,91. doi: 10.15886/j.cnki.rdswxb.2014.01.003
shu

Two-dimensional Polyacrylamide Gel Electrophoresis Techniques for Proteomic Research of Gluconacetobacter

doi: 10.15886/j.cnki.rdswxb.2014.01.003

  • 1. College of Food Sciences, Hainan University, Haikou 570228, China;

  • 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

  • Received Date: 2014-02-10
  • The protein extraction,isoelectric point of distribution and protein purification of the proteins of Gluconacetobacter,a stain produced bacterial cellulose,were tried and optimized to establish a set of two-dimensional polyacrylamide gel electrophoresis（ 2DE） techniques for proteomic analysis of Gluconacetobacter. The results showed that Gluconacetobacter proteins were mainly distributed in the range of pI 4- 7. The 2D Clean-up kit purification after ultrafiltration concentration gave better protein separation effect. Under Coomassie brilliant blue G250 condition,（ 1037 ±65） protein points were isolated.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article views(7) PDF downloads(0) Cited by()

Proportional views
Related

Two-dimensional Polyacrylamide Gel Electrophoresis Techniques for Proteomic Research of Gluconacetobacter

doi: 10.15886/j.cnki.rdswxb.2014.01.003
  • 1. College of Food Sciences, Hainan University, Haikou 570228, China;
  • 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received Date: 2014-02-10

Abstract: The protein extraction,isoelectric point of distribution and protein purification of the proteins of Gluconacetobacter,a stain produced bacterial cellulose,were tried and optimized to establish a set of two-dimensional polyacrylamide gel electrophoresis（ 2DE） techniques for proteomic analysis of Gluconacetobacter. The results showed that Gluconacetobacter proteins were mainly distributed in the range of pI 4- 7. The 2D Clean-up kit purification after ultrafiltration concentration gave better protein separation effect. Under Coomassie brilliant blue G250 condition,（ 1037 ±65） protein points were isolated.

YANG Xuan, LI Jun, LI Congfa, HAO Junran, XU Wentao, LIU Sixin. Two-dimensional Polyacrylamide Gel Electrophoresis Techniques for Proteomic Research of Gluconacetobacter[J]. Journal of Tropical Biology, 2014, 5(1): 73-77,91. doi: 10.15886/j.cnki.rdswxb.2014.01.003
Citation: YANG Xuan, LI Jun, LI Congfa, HAO Junran, XU Wentao, LIU Sixin. Two-dimensional Polyacrylamide Gel Electrophoresis Techniques for Proteomic Research of Gluconacetobacter[J]. Journal of Tropical Biology, 2014, 5(1): 73-77,91. doi: 10.15886/j.cnki.rdswxb.2014.01.003
shu

    HTML

Catalog

    Copyright © 《Journal of Tropical Biology》Editorial Board

    Address: 58 Renmin Avenue, Haikou, Hainan China Pos: 100101Tel: 0898-66281595

    Supported by: Beijing Renhe Information Technology Co. Ltd

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return