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Volume 4 Issue 3
Aug.  2013
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(3): 220-224

YANG Qin, LUO Lijuan. Accurate and Rapid Identification of Colletotrichum from Stylosanthes spp.[J]. Journal of Tropical Biology, 2013, 4(3): 220-224. doi: 10.15886/j.cnki.rdswxb.2013.03.006
Citation: YANG Qin, LUO Lijuan. Accurate and Rapid Identification of Colletotrichum from Stylosanthes spp.[J]. Journal of Tropical Biology, 2013, 4(3): 220-224. doi: 10.15886/j.cnki.rdswxb.2013.03.006
shu

Accurate and Rapid Identification of Colletotrichum from Stylosanthes spp.

doi: 10.15886/j.cnki.rdswxb.2013.03.006

  • 1. National Key Laboratory for Sustainable Utilization of Tropical Biological Resources in Hainan, Haikou 571101, China;

  • 2. College of Agronomy, Hainan University, Haikou 571101, China

  • Received Date: 2013-06-04
  • Anthracnose is the main plant disease of Stylosanthes. Identification of pathogen of the disease is particularly important. Two pathogenic strains of anthracnose were separated by using conventional tissue isolation method,and the rDNA-ITS of the pathogens were amplified by using universal primers ITS1/ITS4. The electrophoresis results showed that the target band was 500 bp approximately. Bioinformatics analysis showed that the two strains were clustered into Colletotrichum gloeosporioides and had 99% homology. Five Stylo species were inoculated with the two pathogenic strains,and the result showed that these 5 stylo species were different in tolerance to anthracnose.
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Accurate and Rapid Identification of Colletotrichum from Stylosanthes spp.

doi: 10.15886/j.cnki.rdswxb.2013.03.006
  • 1. National Key Laboratory for Sustainable Utilization of Tropical Biological Resources in Hainan, Haikou 571101, China;
  • 2. College of Agronomy, Hainan University, Haikou 571101, China
  • Received Date: 2013-06-04

Abstract: Anthracnose is the main plant disease of Stylosanthes. Identification of pathogen of the disease is particularly important. Two pathogenic strains of anthracnose were separated by using conventional tissue isolation method,and the rDNA-ITS of the pathogens were amplified by using universal primers ITS1/ITS4. The electrophoresis results showed that the target band was 500 bp approximately. Bioinformatics analysis showed that the two strains were clustered into Colletotrichum gloeosporioides and had 99% homology. Five Stylo species were inoculated with the two pathogenic strains,and the result showed that these 5 stylo species were different in tolerance to anthracnose.

YANG Qin, LUO Lijuan. Accurate and Rapid Identification of Colletotrichum from Stylosanthes spp.[J]. Journal of Tropical Biology, 2013, 4(3): 220-224. doi: 10.15886/j.cnki.rdswxb.2013.03.006
Citation: YANG Qin, LUO Lijuan. Accurate and Rapid Identification of Colletotrichum from Stylosanthes spp.[J]. Journal of Tropical Biology, 2013, 4(3): 220-224. doi: 10.15886/j.cnki.rdswxb.2013.03.006
shu

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