Rapid Propagation of Hedychium flavum Roxb.

Key words: 
• Hedychium flavum /
• tuber /
• rapid propagation

Abstract: Shoots newly sprouting from underground rhizome of Hedychium flavum Roxb. ere used as explants for rapid propagation. The explants were sterilized,induced into clustered shoots,generated into plantlets and rooted on different media,and then transferred onto the mixed substrates of sand and surface soil for culture. The explants sterilized with HgCl 2 （1 g·L^-1 ） for 12 min gave best result. The optimal medium for proliferation of clustered shoots was 3/4MS+6-BA3.5 mg·L^-1+CH 1 000 mg·L^-1 +sugar 30 g·L^-1. On this medium clustered shoots were subcultured for 25 days as a cycle at a proliferation coefficient of 5. 83. The plantlets were best rooted on the rooting medium of 1/2MS + NAA 0. 5 mg·L- 1, producing upto 9. 17 roots/plantlet with an average root length of 2. 73 cm,and the rooted plantlets grew robust and 5. 43 cm tall by average. The rooted plantlets transferred onto the substrate of river sand 50% + surface soil 50% （V ︰ V） for culture gave 91. 67% survival after 25 days of transfer and grew well.