Establishment of Plant Regeneration System for Highland Barley （Hordeum vulgare var. nudum）

Key words: 
• Hordeum vulgare var. nudum /
• β-glucan /
• plant regeneration

Abstract: For highland barley plant regeneration,mature embryos selected from 10 popularized varieties of high-land barley （Hordeum vulgare L. var. nudum Hook. f. ） in Qinghai-Tibet Plateau were used as explants. A plant regeneration system was successfully established via regular tissue culture procedure. In the plant regeneration system the callus inducing rate for the embryos of the 10 barley varieties was higher when cultured in the MS medium at the presence of L-Proline （1 g·L^-1 ） + Casein hydrolyzate （8 g·L^-1 ） + 2,4-D （mg·L^-1 ）, and adventitious bud inducing rate was higher in the MS medium supplemented with L-Proline （1 g·L^-1 ） + Casein hydrolyzate （8 g·L^-1 ）. The plantlets were induced to root in the 1/2 MS rooting medium.