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Volume 4 Issue 2
Apr.  2025
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(2): 124-128

LIN Shaodong, CHE Haiyan. PCR Detection of Whitefly-transmitted Geminivirus of Cucumis melo var. Saccharinus in Ledong,Hainan Province[J]. Journal of Tropical Biology, 2013, 4(2): 124-128. doi: 10.15886/j.cnki.rdswxb.2013.02.006
Citation: LIN Shaodong, CHE Haiyan. PCR Detection of Whitefly-transmitted Geminivirus of Cucumis melo var. Saccharinus in Ledong,Hainan Province[J]. Journal of Tropical Biology, 2013, 4(2): 124-128. doi: 10.15886/j.cnki.rdswxb.2013.02.006
shu

PCR Detection of Whitefly-transmitted Geminivirus of Cucumis melo var. Saccharinus in Ledong,Hainan Province

doi: 10.15886/j.cnki.rdswxb.2013.02.006

  • 1. College of Environment and Plant Protection, Hainan University, Haikou 570228, China;

  • 2. Environment and Protection Institute, CATAS, Haikou 571101, China;

  • 3. Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, China

  • Received Date: 2013-03-22
  • Four samples of diseased Cucumis melo var. Saccharinus showing stunting,small leaf,mosaic symptom were collected in Ledong,Hainan Province,China. PCR product of expected size was amplified from three samples using the begomovirus degenerate primer pair PA/PB,cloned and sequenced. The size of the PCR product was 509 bp. Phylogenetic analysis showed that HA1 clustered with SLCPHV-[Taiwan]and SLCPHV while HA3 and HA4 clustered with SLCCNV-[Melon]and SLCCNV-[Hn61],which indicated that the three samples of C. melo var. Saccharinus were infected likely with different species of geminiviruses.
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PCR Detection of Whitefly-transmitted Geminivirus of Cucumis melo var. Saccharinus in Ledong,Hainan Province

doi: 10.15886/j.cnki.rdswxb.2013.02.006
  • 1. College of Environment and Plant Protection, Hainan University, Haikou 570228, China;
  • 2. Environment and Protection Institute, CATAS, Haikou 571101, China;
  • 3. Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, China
  • Received Date: 2013-03-22

Abstract: Four samples of diseased Cucumis melo var. Saccharinus showing stunting,small leaf,mosaic symptom were collected in Ledong,Hainan Province,China. PCR product of expected size was amplified from three samples using the begomovirus degenerate primer pair PA/PB,cloned and sequenced. The size of the PCR product was 509 bp. Phylogenetic analysis showed that HA1 clustered with SLCPHV-[Taiwan]and SLCPHV while HA3 and HA4 clustered with SLCCNV-[Melon]and SLCCNV-[Hn61],which indicated that the three samples of C. melo var. Saccharinus were infected likely with different species of geminiviruses.

LIN Shaodong, CHE Haiyan. PCR Detection of Whitefly-transmitted Geminivirus of Cucumis melo var. Saccharinus in Ledong,Hainan Province[J]. Journal of Tropical Biology, 2013, 4(2): 124-128. doi: 10.15886/j.cnki.rdswxb.2013.02.006
Citation: LIN Shaodong, CHE Haiyan. PCR Detection of Whitefly-transmitted Geminivirus of Cucumis melo var. Saccharinus in Ledong,Hainan Province[J]. Journal of Tropical Biology, 2013, 4(2): 124-128. doi: 10.15886/j.cnki.rdswxb.2013.02.006
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