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LIAO Wenbin, PENG Ming. Gene Cloning of DELLA Protein from Cassava and Its Expression Patterns Under Drought Stress[J]. Journal of Tropical Biology, 2012, 3(4): 298-304. doi: 10.15886/j.cnki.rdswxb.2012.04.004
Citation:
LIAO Wenbin, PENG Ming. Gene Cloning of DELLA Protein from Cassava and Its Expression Patterns Under Drought Stress[J]. Journal of Tropical Biology, 2012, 3(4): 298-304. doi: 10.15886/j.cnki.rdswxb.2012.04.004
Gene Cloning of DELLA Protein from Cassava and Its Expression Patterns Under Drought Stress
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Ministry of Agriculture Key Laboratory for Biology and Genetic Resources of Tropical Crops, Haikou 571101, China
Received Date: 2012-10-10
Abstract
The GA signaling pathway is regulated by the DELLA proteins.A cassava nucleotide with high sequence homology to Arabidopsis thaliana GAI（AtGAI） was identified in the JGI database with the NCBI BLAST program.This nucleotide,named MeGAI,has a full length of 1 857 bp and contained genomic coding sequences.Sequence comparisons between the MeGAI sequence and all of DELLA proteins in Arabidopsis indicated that MeGAI also has similar conserved domains to the DELLA proteins of the Arabidopsis,such as DELLA domain,VHYNP domain,POLY（S /T） domain,NLS domain,VHVID domain,leu zipper domain,GRAS domain.All these domains indicted that MeGAI is an ortholog of AtGAI.Analysis of expression patterns of this gene in response to drought stress treatment showed that this gene is down regulated.Moreover,analysis of the expression patterns of the GA20ox gene,a key gene on GA biosynthesis process,indicated that the GA20ox gene has similar expression patterns to MeGAI.All these results give the clues to possible involvement of GA signal in resistance to drought stress.
HARTWECK L M,OISZWSKI N E.Rice GIBBERELLIN INSENSITIVE DWARF1is a gibberellin receptor that illuminates and raises questions about GA signaling[J].Plant cell,2006,18(2):278-282.
[6]
THOMAS S G,SUN T P.Update on Gibberellin Signaling.A Tale of the Tall and the Short[J].Plant Physiology,2004,135(2):668-676.
[7]
LIAO W B,RUAN M B,CUI B M,et al.Isolation and Characterization of a GAI/RGA-like gene from Gossypium hirsutum[J].Plant Growth Regulation,2009,58(1):34-45.
[8]
HUSSAIN A,PENG J R.DELLA Proteins and GA Signalling in Arabidopsis[J].J Plant Growth Regul,2003,22:134-140.
[9]
FU X,SUDHAKAR D,PENG J,et al.Expression of Arabidopsis GAI in transgenic rice represses multiple gibberellin respon-ses[J].Plant Cell,2001,13(8):1791-802.
[10]
ZENTELLA W,ZHANG Z L,PARK M,et al.Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Ar-abidopsis[J].The Plant Cell,2007(19):3037-305.
[11]
KANEKO M,ITOH H,INUKAI Y,et al.Where do gibberellin biosynthesis and signaling occur in rice plants?[J].Plant J,2003,34:1-12.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Ministry of Agriculture Key Laboratory for Biology and Genetic Resources of Tropical Crops, Haikou 571101, China
Abstract: The GA signaling pathway is regulated by the DELLA proteins.A cassava nucleotide with high sequence homology to Arabidopsis thaliana GAI（AtGAI） was identified in the JGI database with the NCBI BLAST program.This nucleotide,named MeGAI,has a full length of 1 857 bp and contained genomic coding sequences.Sequence comparisons between the MeGAI sequence and all of DELLA proteins in Arabidopsis indicated that MeGAI also has similar conserved domains to the DELLA proteins of the Arabidopsis,such as DELLA domain,VHYNP domain,POLY（S /T） domain,NLS domain,VHVID domain,leu zipper domain,GRAS domain.All these domains indicted that MeGAI is an ortholog of AtGAI.Analysis of expression patterns of this gene in response to drought stress treatment showed that this gene is down regulated.Moreover,analysis of the expression patterns of the GA20ox gene,a key gene on GA biosynthesis process,indicated that the GA20ox gene has similar expression patterns to MeGAI.All these results give the clues to possible involvement of GA signal in resistance to drought stress.
LIAO Wenbin, PENG Ming. Gene Cloning of DELLA Protein from Cassava and Its Expression Patterns Under Drought Stress[J]. Journal of Tropical Biology, 2012, 3(4): 298-304. doi: 10.15886/j.cnki.rdswxb.2012.04.004
Citation:
LIAO Wenbin, PENG Ming. Gene Cloning of DELLA Protein from Cassava and Its Expression Patterns Under Drought Stress[J]. Journal of Tropical Biology, 2012, 3(4): 298-304. doi: 10.15886/j.cnki.rdswxb.2012.04.004
HARTWECK L M,OISZWSKI N E.Rice GIBBERELLIN INSENSITIVE DWARF1is a gibberellin receptor that illuminates and raises questions about GA signaling[J].Plant cell,2006,18(2):278-282.
[6]
THOMAS S G,SUN T P.Update on Gibberellin Signaling.A Tale of the Tall and the Short[J].Plant Physiology,2004,135(2):668-676.
[7]
LIAO W B,RUAN M B,CUI B M,et al.Isolation and Characterization of a GAI/RGA-like gene from Gossypium hirsutum[J].Plant Growth Regulation,2009,58(1):34-45.
[8]
HUSSAIN A,PENG J R.DELLA Proteins and GA Signalling in Arabidopsis[J].J Plant Growth Regul,2003,22:134-140.
[9]
FU X,SUDHAKAR D,PENG J,et al.Expression of Arabidopsis GAI in transgenic rice represses multiple gibberellin respon-ses[J].Plant Cell,2001,13(8):1791-802.
[10]
ZENTELLA W,ZHANG Z L,PARK M,et al.Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Ar-abidopsis[J].The Plant Cell,2007(19):3037-305.
[11]
KANEKO M,ITOH H,INUKAI Y,et al.Where do gibberellin biosynthesis and signaling occur in rice plants?[J].Plant J,2003,34:1-12.
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LIAO Wenbin, PENG Ming. Gene Cloning of DELLA Protein from Cassava and Its Expression Patterns Under Drought Stress[J]. Journal of Tropical Biology, 2012, 3(4): 298-304. doi: 10.15886/j.cnki.rdswxb.2012.04.004
LIAO Wenbin, PENG Ming. Gene Cloning of DELLA Protein from Cassava and Its Expression Patterns Under Drought Stress[J]. Journal of Tropical Biology, 2012, 3(4): 298-304. doi: 10.15886/j.cnki.rdswxb.2012.04.004
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