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Volume 3 Issue 3
Sep.  2012
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Article Navigation >  Journal of Tropical Biology  > 2012  >  3(3): 271-275

XIA Yun, TAN Wenli, CHEN Yinhua, HUANG Xiaolong, XIE Jun, HUANG Dongyi. Rapid Propagation of Dioscorea alata L.[J]. Journal of Tropical Biology, 2012, 3(3): 271-275. doi: 10.15886/j.cnki.rdswxb.2012.03.011
Citation: XIA Yun, TAN Wenli, CHEN Yinhua, HUANG Xiaolong, XIE Jun, HUANG Dongyi. Rapid Propagation of Dioscorea alata L.[J]. Journal of Tropical Biology, 2012, 3(3): 271-275. doi: 10.15886/j.cnki.rdswxb.2012.03.011
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Rapid Propagation of Dioscorea alata L.

doi: 10.15886/j.cnki.rdswxb.2012.03.011

  • College of Agronomy, Hainan University, Haikou 570228, China

  • Received Date: 2012-05-15
  • Dioscorea alata L. were mass propagated through tissue culture on different MS media with the nodal stem segments as explants. The explants were induced to form axillary buds at an initiation rate of 100% when cultured on the MS medium without any auxins. The initiated nodal shoots were rapidly multiplied in the subculture basic medium supplemented with 80 mg·L-1 AD （adenine sulfate） and 4 times of nodal shoots could be produced at day 50 of subculture. Adventitious buds were induced from the nodal segments subcultured in the basic medium supplemented with 6-BA 1.0 mg·L-1,NAA0.1 mg·L-1 and 80 mg·L-1 AD, and produced robust shoots when cultured on the medium supplemented with 80 mg·L-1 AD. The adventitious shoots were rooted on the MS medium containing NAA0.1 mg·L-1 to form complete plants, and the rooting rate was 100%. The rooted plantlets were transplanted onto a mixed substrate of garden soil, sand and plant ash（V:V:V=2:2:1） and resulted in 88.3% survival.
  • [1] 吴志刚, 魏余煌, 陶正明, 等. 紫肉参薯高产综合栽培技术研究[J]. 浙江农业学报, 2010, 22(1):36-39.
    [2] WANASUNDERA J P D, RAVINDRAN G. Nutrition assessment of yam tuber[J]. Plant Foods for Human Nutrition, 1994, 46:33-39.
    [3] 梁小娟, 杜伟锋, 张云, 等. 参薯研究进展[J]. 中华中医药学刊, 2011, 29(5):1085-1087.
    [4] 周双清, 胡新文, 黄东益, 等. 参薯组织培养的初步研究[J]. 中国热带农业, 2011, 42(5):85-87.
    [5] BELARMINO M M, GONZALES J R. Somatic embryogenesis and plant regeneration in purple food yam(Dioscorea alata)[J]. Annals of Tropical Research, 2008, 30(2):22-33.
    [6] TWYFORD C T, MANTELL S H. Production of somatic and plantlets from root cells of the greater yam[J]. Plant Cell, Tissue and Culture, 1996, 46:17-26.
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Rapid Propagation of Dioscorea alata L.

doi: 10.15886/j.cnki.rdswxb.2012.03.011
  • College of Agronomy, Hainan University, Haikou 570228, China
  • Received Date: 2012-05-15

Abstract: Dioscorea alata L. were mass propagated through tissue culture on different MS media with the nodal stem segments as explants. The explants were induced to form axillary buds at an initiation rate of 100% when cultured on the MS medium without any auxins. The initiated nodal shoots were rapidly multiplied in the subculture basic medium supplemented with 80 mg·L-1 AD （adenine sulfate） and 4 times of nodal shoots could be produced at day 50 of subculture. Adventitious buds were induced from the nodal segments subcultured in the basic medium supplemented with 6-BA 1.0 mg·L-1,NAA0.1 mg·L-1 and 80 mg·L-1 AD, and produced robust shoots when cultured on the medium supplemented with 80 mg·L-1 AD. The adventitious shoots were rooted on the MS medium containing NAA0.1 mg·L-1 to form complete plants, and the rooting rate was 100%. The rooted plantlets were transplanted onto a mixed substrate of garden soil, sand and plant ash（V:V:V=2:2:1） and resulted in 88.3% survival.

XIA Yun, TAN Wenli, CHEN Yinhua, HUANG Xiaolong, XIE Jun, HUANG Dongyi. Rapid Propagation of Dioscorea alata L.[J]. Journal of Tropical Biology, 2012, 3(3): 271-275. doi: 10.15886/j.cnki.rdswxb.2012.03.011
Citation: XIA Yun, TAN Wenli, CHEN Yinhua, HUANG Xiaolong, XIE Jun, HUANG Dongyi. Rapid Propagation of Dioscorea alata L.[J]. Journal of Tropical Biology, 2012, 3(3): 271-275. doi: 10.15886/j.cnki.rdswxb.2012.03.011
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