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Volume 3 Issue 3
Sep.  2012
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Article Navigation >  Journal of Tropical Biology  > 2012  >  3(3): 258-260,270

LI Hongyang, LIU Fei, ZHANG Fengqin, LI Xiaolong, LIU Guomin. Supercritical Fluid CO2 Extraction and Purification of Urosolic Acid from Ilex kudingcha C.J.Tseng[J]. Journal of Tropical Biology, 2012, 3(3): 258-260,270. doi: 10.15886/j.cnki.rdswxb.2012.03.005
Citation: LI Hongyang, LIU Fei, ZHANG Fengqin, LI Xiaolong, LIU Guomin. Supercritical Fluid CO2 Extraction and Purification of Urosolic Acid from Ilex kudingcha C.J.Tseng[J]. Journal of Tropical Biology, 2012, 3(3): 258-260,270. doi: 10.15886/j.cnki.rdswxb.2012.03.005
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Supercritical Fluid CO2 Extraction and Purification of Urosolic Acid from Ilex kudingcha C.J.Tseng

doi: 10.15886/j.cnki.rdswxb.2012.03.005

  • 1. Key Laboratory of Tropical Biological Resources. MOE, Hainan University, Haikou 570228, China;

  • 2. Kudingcha Research Institute, Hainan University, Haikou 570228, China;

  • 3. Hunan Key Lab of Green Packaging and Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China

  • Received Date: 2012-03-15
  • Ursolic acid was extracted supercritically from Ilex kudingcha C.J.Tseng with fluid CO2. The extract liquid was purified with acid and alkali and detected with high performance liquid chromatography （HPLC）. Optimal extracting conditions resulted to be absolute ethanol as entrainer, CO2 flow rate at 20 L·h-1, extracting temperature at 45 ℃, extracting pressure at 30 MPa and extracting time for 3 h, and the extraction rate was upto 0.453%. Moreover, crude products of ursolic acid with a purity of 21.44% were produced finally. This extraction technology is superior to the traditional solvent extraction of ursolic acid, and suitable for industrial production.
  • [1] 张镜澄.超临界流体萃取[M].北京:化学工业出版社,2000:86.
    [2] 高福成.现代食品工程高新科技[M].北京:中国工业出版社,1997:394.
    [3] 蒋胜岩,赵良忠,余有贵,等.夏枯草叶熊果酸超临界流体萃取工艺的研究[J].食品科学,2008,29(11):294-297.
    [4] 刘艳辉.正交设计法优化从车前草中萃取熊果酸的工艺研究[J].化工时刊,2008,22(3):22-24.
    [5] 任秀莲,周春山,魏琦峰,等.反相高效液相色谱法测定苦丁茶中的熊果酸[J].光谱实验室,2004,21(4):693-697.
    [6] 袁珂,孙伟,张晓明.超临界二氧化碳萃取冬凌草中熊果酸的研究[J].林产化学与工业,2006,26(2):131-134.
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Supercritical Fluid CO2 Extraction and Purification of Urosolic Acid from Ilex kudingcha C.J.Tseng

doi: 10.15886/j.cnki.rdswxb.2012.03.005
  • 1. Key Laboratory of Tropical Biological Resources. MOE, Hainan University, Haikou 570228, China;
  • 2. Kudingcha Research Institute, Hainan University, Haikou 570228, China;
  • 3. Hunan Key Lab of Green Packaging and Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China
  • Received Date: 2012-03-15

Abstract: Ursolic acid was extracted supercritically from Ilex kudingcha C.J.Tseng with fluid CO2. The extract liquid was purified with acid and alkali and detected with high performance liquid chromatography （HPLC）. Optimal extracting conditions resulted to be absolute ethanol as entrainer, CO2 flow rate at 20 L·h-1, extracting temperature at 45 ℃, extracting pressure at 30 MPa and extracting time for 3 h, and the extraction rate was upto 0.453%. Moreover, crude products of ursolic acid with a purity of 21.44% were produced finally. This extraction technology is superior to the traditional solvent extraction of ursolic acid, and suitable for industrial production.

LI Hongyang, LIU Fei, ZHANG Fengqin, LI Xiaolong, LIU Guomin. Supercritical Fluid CO2 Extraction and Purification of Urosolic Acid from Ilex kudingcha C.J.Tseng[J]. Journal of Tropical Biology, 2012, 3(3): 258-260,270. doi: 10.15886/j.cnki.rdswxb.2012.03.005
Citation: LI Hongyang, LIU Fei, ZHANG Fengqin, LI Xiaolong, LIU Guomin. Supercritical Fluid CO2 Extraction and Purification of Urosolic Acid from Ilex kudingcha C.J.Tseng[J]. Journal of Tropical Biology, 2012, 3(3): 258-260,270. doi: 10.15886/j.cnki.rdswxb.2012.03.005
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