Advance Search

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 3 Issue 2
Jun.  2012
Turn off MathJax
Article Contents
Article Navigation >  Journal of Tropical Biology  > 2012  >  3(2): 135-137

FAN Jie, AN Fei-fei, ZHENG Feng. Chromosome Observation Method for Cassava Tender Leaves[J]. Journal of Tropical Biology, 2012, 3(2): 135-137. doi: 10.15886/j.cnki.rdswxb.2012.02.012
Citation: FAN Jie, AN Fei-fei, ZHENG Feng. Chromosome Observation Method for Cassava Tender Leaves[J]. Journal of Tropical Biology, 2012, 3(2): 135-137. doi: 10.15886/j.cnki.rdswxb.2012.02.012
shu

Chromosome Observation Method for Cassava Tender Leaves

doi: 10.15886/j.cnki.rdswxb.2012.02.012

  • 1. College of Agronomy, Hainan University, Haikou 571737, China;

  • 2. Tropical Crops Genetic Resources Institute, CATAS, Danzhou 571737, China

  • Received Date: 2012-03-07
  • Modified phenol fuchsin squashing method was used to prepare chromosome specimens of tender leaves of polypoidy cassava SC 124.The tender leaves were pretreated with 0.002 mol.L-1 8-hydroxyquinoline at three different temperatures for different time by using different solution methods and sampled from different parts of the plant.The tender leaves collected 1.5-2 cm long in the morning at 9:00 am-10:00 am were pretreated with 0.002 mol.L-1 the 8-hydroxyquinoline at 4 C for 4 h,and then put in 1 mol.L-1 hydrochloric acid solution at 25 C for 1 h for maceration,and the mesophyll in the central part of the tender leaves were stained with modified phenol suchsin and then squashed for observation.This method produced a well dispersed,clear chromosome image.
  • [1] JENNINGS D L.Cassava Manihot esculenta (Euphorbiaceae) [J].African Agri J, 1994(10):34-39.
    [2] 陈显双, 韦丽君, 田益农, 等.木薯多倍体植株的诱导研究[J].热带农业科学, 2008, 28(1):17-20.
    [3] 冯斗, 王建岭, 席世丽, 等.木薯根尖染色体的观察技术[J].安徽农业科学, 2008, 36(9):3711-3712.
    [4] 陈显双, 韦丽君, 杨琴, 等.木薯染色体数目的鉴定技术[J].广西热带农业, 2009(4):1-3.
    [5] 曹芳, 阮金玉, 郑惠宝, 等.取材时间和预处理方法对大蒜有丝分裂指数的影响[J].漳州师范学院学报:自然科学版, 2010(4):113-117.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article views(15) PDF downloads(0) Cited by()

Proportional views
Related

Chromosome Observation Method for Cassava Tender Leaves

doi: 10.15886/j.cnki.rdswxb.2012.02.012
  • 1. College of Agronomy, Hainan University, Haikou 571737, China;
  • 2. Tropical Crops Genetic Resources Institute, CATAS, Danzhou 571737, China
  • Received Date: 2012-03-07

Abstract: Modified phenol fuchsin squashing method was used to prepare chromosome specimens of tender leaves of polypoidy cassava SC 124.The tender leaves were pretreated with 0.002 mol.L-1 8-hydroxyquinoline at three different temperatures for different time by using different solution methods and sampled from different parts of the plant.The tender leaves collected 1.5-2 cm long in the morning at 9:00 am-10:00 am were pretreated with 0.002 mol.L-1 the 8-hydroxyquinoline at 4 C for 4 h,and then put in 1 mol.L-1 hydrochloric acid solution at 25 C for 1 h for maceration,and the mesophyll in the central part of the tender leaves were stained with modified phenol suchsin and then squashed for observation.This method produced a well dispersed,clear chromosome image.

FAN Jie, AN Fei-fei, ZHENG Feng. Chromosome Observation Method for Cassava Tender Leaves[J]. Journal of Tropical Biology, 2012, 3(2): 135-137. doi: 10.15886/j.cnki.rdswxb.2012.02.012
Citation: FAN Jie, AN Fei-fei, ZHENG Feng. Chromosome Observation Method for Cassava Tender Leaves[J]. Journal of Tropical Biology, 2012, 3(2): 135-137. doi: 10.15886/j.cnki.rdswxb.2012.02.012
shu

    HTML

Reference (5)

Catalog

    Copyright © 《Journal of Tropical Biology》Editorial Board

    Address: 58 Renmin Avenue, Haikou, Hainan China Pos: 100101Tel: 0898-66281595

    Supported by: Beijing Renhe Information Technology Co. Ltd

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return