WEI Wei, YANG La-ying, ZHOU Duan-yong, XIE De-xiao, HUANG Xiao-juan, LIU Yi-xian, HUANG Jun-sheng. Cloning and Sequence Analysis of fgb2 Gene in Fusarium oxysporum f.sp.cubense[J]. Journal of Tropical Biology, 2012, 3(2): 138-141. doi: 10.15886/j.cnki.rdswxb.2012.02.010
| Citation:
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WEI Wei, YANG La-ying, ZHOU Duan-yong, XIE De-xiao, HUANG Xiao-juan, LIU Yi-xian, HUANG Jun-sheng. Cloning and Sequence Analysis of fgb2 Gene in Fusarium oxysporum f.sp.cubense[J]. Journal of Tropical Biology, 2012, 3(2): 138-141. doi: 10.15886/j.cnki.rdswxb.2012.02.010
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Cloning and Sequence Analysis of fgb2 Gene in Fusarium oxysporum f.sp.cubense
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WEI Wei1,2
,
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YANG La-ying2
,
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ZHOU Duan-yong1,2
,
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XIE De-xiao1,2
,
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HUANG Xiao-juan1,2
,
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LIU Yi-xian1,2
,
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HUANG Jun-sheng2
,
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1. College of Agronomy, Hainan University, Haikou 570228, China;
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2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Danzhou 571737, China
- Received Date: 2012-03-04
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Abstract
It is very important to know the roles of fgb2 genes in invasion process of Fusarium oxysporum f.sp.cubense（FOC） into banana and its relationship in pathogenic difference between the races 1 and 4 of FOC attacking the same banana cultivar.The fgb2 genes were amplified by using Polymerase Chain Reaction and Reverse Transcription Polymerase Chain Reaction,and the amplified products were then sequenced and aligned with DNAStar Meglignment software.The functions of the proteins encoded by fgb2 genes were also analyzed.The results revealed that the open reading frames of fgb2 genes from races of FOC were 1 466 bp and 1 452 bp,encoding 299 and 316 amino acids.FOC race 1 showed a 96.33% similarity of fgb2 gene to FOC race 4.The sequences of fgb2 genes and amino acid sequences of the two FOC races showed no relationship between the pathogenic difference and the fgb2 gene.These results would help to understand the function of fgb2 genes in followup research activities.
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