Test of Tumor-inhibition of Benzyl Isothiocyanate（BITC）in Papaya Seed

ZHOU Li; LI Zeyou; SHEN Wen-tao; JIN Yun-kui; ZHOU Peng

doi:10.15886/j.cnki.rdswxb.2012.02.006

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Benzyl isothiocyanate（BITC） was extracted from papaya seeds（Carica papaya L.） and used to determine its inhibitory effect on the cell growth of 6 common cancers in vitro including liver cancer by using the MTT method.The results showed the papaya seed extract with BITC at the concentration of up to 5 μmol.L^-1（equivalent to 0.745 mg.L^-1） inhibited more than 70% of the cell growth of a human liver cancer cell line HepG2,a lung cancer cell line A549,a breast cancer cell line MCF-7,a colon cancer cell line HCT-8,a cervical cancer cell line Hela and a prostate cancer cell line DU-145.Of them the HepG2 liver cancer cells were the most sensitive to BITC which gave an 87% inhibition over the cells at the concentration 2.5 μmol.L^-1.HCT-8 colon cancer cells had a similar inhibition rate when treated with BITC at both 5.0 and 10 μmol.L^-1.This showed that higher concentration of BITC in the extract of papaya seeds was similar to the lower concentration（5.0 μmol.L^-1） in inhibition of the growth of the cancer cells.

Test of Tumor-inhibition of Benzyl Isothiocyanate（BITC）in Papaya Seed

1. Hainan Nongken Middle School, Haikou 570226, China;

2. Hainan Medical University, Haikou 571101, China;

3. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Received Date: 2012-03-05

Key words:

Abstract: Benzyl isothiocyanate（BITC） was extracted from papaya seeds（Carica papaya L.） and used to determine its inhibitory effect on the cell growth of 6 common cancers in vitro including liver cancer by using the MTT method.The results showed the papaya seed extract with BITC at the concentration of up to 5 μmol.L^-1（equivalent to 0.745 mg.L^-1） inhibited more than 70% of the cell growth of a human liver cancer cell line HepG2,a lung cancer cell line A549,a breast cancer cell line MCF-7,a colon cancer cell line HCT-8,a cervical cancer cell line Hela and a prostate cancer cell line DU-145.Of them the HepG2 liver cancer cells were the most sensitive to BITC which gave an 87% inhibition over the cells at the concentration 2.5 μmol.L^-1.HCT-8 colon cancer cells had a similar inhibition rate when treated with BITC at both 5.0 and 10 μmol.L^-1.This showed that higher concentration of BITC in the extract of papaya seeds was similar to the lower concentration（5.0 μmol.L^-1） in inhibition of the growth of the cancer cells.

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Reference (20)

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Test of Tumor-inhibition of Benzyl Isothiocyanate（BITC）in Papaya Seed

doi: 10.15886/j.cnki.rdswxb.2012.02.006

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