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Volume 2 Issue 3
Sep.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(3): 230-236

GUO Junhui, ZHANG Jian, CHEN Xiongting, WANG Ying, WU Kunxin, NI Yanyan, HUANG Qin. Studies on Influenc ing Factors of Cassava Regeneration System[J]. Journal of Tropical Biology, 2011, 2(3): 230-236. doi: 10.15886/j.cnki.rdswxb.2011.03.012
Citation: GUO Junhui, ZHANG Jian, CHEN Xiongting, WANG Ying, WU Kunxin, NI Yanyan, HUANG Qin. Studies on Influenc ing Factors of Cassava Regeneration System[J]. Journal of Tropical Biology, 2011, 2(3): 230-236. doi: 10.15886/j.cnki.rdswxb.2011.03.012
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Studies on Influenc ing Factors of Cassava Regeneration System

doi: 10.15886/j.cnki.rdswxb.2011.03.012

  • 1. College of Agriculture, Hainan University, Haikou 570228, China;

  • 2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

  • Received Date: 2011-07-08
  • In this report, Varieties of SC6, SC7, SC8, SC124, NZ188, C3 and C4 were used as experimental materials, several significant factors which influence the regeneration system of cassava were studied. The results indicated that 6-BA had a significant inhibitory effect on apical dominance of cassava, the higher the concentration was, the stronger the inhibition effects was; 2,4-D and picloram had almost equal effects for promoting somatic embryogenesis, in the process of induction of primary somatic embryos, embryogenesis rate of all breeds reached 100%, except SC7 remained a low embryogenesis rate of secondary somatic embryos, embryogenesis rate of secondary somatic embryos of other varieties went up to 90%; the ripening time of somatic embryos had a great influence on the organogenesis of cotyledon, frequency of cotyledon organogenesis was higher around 2 weeks after embryo maturation and the induction frequency could be more than 70%; the somatic embryogenesis ratio and cotyledon organogenesis frequencies were very different among distinct genotypes,SC8 and NZ188 cultivar had the highest and second-highest frequency respectively,SC7 cultivar displayed the lowest frequency; The addition of AgNO3（5 mg·L-1） into subculture could help improving the organogenesis capacity of mature cotyledonary.
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Studies on Influenc ing Factors of Cassava Regeneration System

doi: 10.15886/j.cnki.rdswxb.2011.03.012
  • 1. College of Agriculture, Hainan University, Haikou 570228, China;
  • 2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
  • Received Date: 2011-07-08

Abstract: In this report, Varieties of SC6, SC7, SC8, SC124, NZ188, C3 and C4 were used as experimental materials, several significant factors which influence the regeneration system of cassava were studied. The results indicated that 6-BA had a significant inhibitory effect on apical dominance of cassava, the higher the concentration was, the stronger the inhibition effects was; 2,4-D and picloram had almost equal effects for promoting somatic embryogenesis, in the process of induction of primary somatic embryos, embryogenesis rate of all breeds reached 100%, except SC7 remained a low embryogenesis rate of secondary somatic embryos, embryogenesis rate of secondary somatic embryos of other varieties went up to 90%; the ripening time of somatic embryos had a great influence on the organogenesis of cotyledon, frequency of cotyledon organogenesis was higher around 2 weeks after embryo maturation and the induction frequency could be more than 70%; the somatic embryogenesis ratio and cotyledon organogenesis frequencies were very different among distinct genotypes,SC8 and NZ188 cultivar had the highest and second-highest frequency respectively,SC7 cultivar displayed the lowest frequency; The addition of AgNO3（5 mg·L-1） into subculture could help improving the organogenesis capacity of mature cotyledonary.

GUO Junhui, ZHANG Jian, CHEN Xiongting, WANG Ying, WU Kunxin, NI Yanyan, HUANG Qin. Studies on Influenc ing Factors of Cassava Regeneration System[J]. Journal of Tropical Biology, 2011, 2(3): 230-236. doi: 10.15886/j.cnki.rdswxb.2011.03.012
Citation: GUO Junhui, ZHANG Jian, CHEN Xiongting, WANG Ying, WU Kunxin, NI Yanyan, HUANG Qin. Studies on Influenc ing Factors of Cassava Regeneration System[J]. Journal of Tropical Biology, 2011, 2(3): 230-236. doi: 10.15886/j.cnki.rdswxb.2011.03.012
shu

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