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Volume 2 Issue 3
Sep.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(3): 204-208

DAI Haiyan, WANG Hua, WEN Shaomin, JIE Na, DENG Fangcheng. Expression of Porphyromonas Gingivalis Fimbrillin Gene in the Fruit of Transgenic Tomato and Its Immunization Assay[J]. Journal of Tropical Biology, 2011, 2(3): 204-208. doi: 10.15886/j.cnki.rdswxb.2011.03.006
Citation: DAI Haiyan, WANG Hua, WEN Shaomin, JIE Na, DENG Fangcheng. Expression of Porphyromonas Gingivalis Fimbrillin Gene in the Fruit of Transgenic Tomato and Its Immunization Assay[J]. Journal of Tropical Biology, 2011, 2(3): 204-208. doi: 10.15886/j.cnki.rdswxb.2011.03.006
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Expression of Porphyromonas Gingivalis Fimbrillin Gene in the Fruit of Transgenic Tomato and Its Immunization Assay

doi: 10.15886/j.cnki.rdswxb.2011.03.006

  • 1. School of Stomatology, Haian Medical College, Haikou 570102, China;

  • 2. Hainan Provincial Key Laboratory of Tropical Medicine, Haian Medical College, Haikou 570102, China

  • Received Date: 2011-06-24
  • In this report, Agrobacterium-mediated transformation of tomato with plasmid pBIE8-CTB-FimA（266-337） containing FimA and CTB gene with E8 promoter were performed, and the immunity of the transgenic tomato fruit as an edible vaccine by oral immunization of mice were determined. The results indicated that the transgenic plants were confirmed by Southern blot and RT-PCR; the fusion proteins from leaf and fruit tissue of transgenic plants were detected by Western blot; specific anti-FimA antibodies in the serum of mouse were detected, all of which suggested that candidate edible plant vaccine was obtained successfully.
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Expression of Porphyromonas Gingivalis Fimbrillin Gene in the Fruit of Transgenic Tomato and Its Immunization Assay

doi: 10.15886/j.cnki.rdswxb.2011.03.006
  • 1. School of Stomatology, Haian Medical College, Haikou 570102, China;
  • 2. Hainan Provincial Key Laboratory of Tropical Medicine, Haian Medical College, Haikou 570102, China
  • Received Date: 2011-06-24

Abstract: In this report, Agrobacterium-mediated transformation of tomato with plasmid pBIE8-CTB-FimA（266-337） containing FimA and CTB gene with E8 promoter were performed, and the immunity of the transgenic tomato fruit as an edible vaccine by oral immunization of mice were determined. The results indicated that the transgenic plants were confirmed by Southern blot and RT-PCR; the fusion proteins from leaf and fruit tissue of transgenic plants were detected by Western blot; specific anti-FimA antibodies in the serum of mouse were detected, all of which suggested that candidate edible plant vaccine was obtained successfully.

DAI Haiyan, WANG Hua, WEN Shaomin, JIE Na, DENG Fangcheng. Expression of Porphyromonas Gingivalis Fimbrillin Gene in the Fruit of Transgenic Tomato and Its Immunization Assay[J]. Journal of Tropical Biology, 2011, 2(3): 204-208. doi: 10.15886/j.cnki.rdswxb.2011.03.006
Citation: DAI Haiyan, WANG Hua, WEN Shaomin, JIE Na, DENG Fangcheng. Expression of Porphyromonas Gingivalis Fimbrillin Gene in the Fruit of Transgenic Tomato and Its Immunization Assay[J]. Journal of Tropical Biology, 2011, 2(3): 204-208. doi: 10.15886/j.cnki.rdswxb.2011.03.006
shu

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