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Volume 2 Issue 2
Jun.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(2): 153-156

GAO Jianming, DAI Zhenzhen, JIANG Chengji, ZHANG Shiqing, CHEN Helong, ZHENG Jinlong, LIU Qiaolian, YI Kexian. Comparison of DNA Extraction Methods of Asparagus[J]. Journal of Tropical Biology, 2011, 2(2): 153-156. doi: 10.15886/j.cnki.rdswxb.2011.02.014
Citation: GAO Jianming, DAI Zhenzhen, JIANG Chengji, ZHANG Shiqing, CHEN Helong, ZHENG Jinlong, LIU Qiaolian, YI Kexian. Comparison of DNA Extraction Methods of Asparagus[J]. Journal of Tropical Biology, 2011, 2(2): 153-156. doi: 10.15886/j.cnki.rdswxb.2011.02.014
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Comparison of DNA Extraction Methods of Asparagus

doi: 10.15886/j.cnki.rdswxb.2011.02.014

  • 1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101;

  • 2. College of Agriculture, Hainan University, Haikou 570228, China

  • Received Date: 2011-04-08
  • In order to find the best DNA extraction method of asparagus,improved SDS,CTAB and kit method were used to extract DNA from Asparagus young leaves and the DNA quality also was tested by UV spectrophotometer,agarose gel electrophoresis and PCR detection.The results showed that the quality of the DNA from the three DNA extraction methods fill the requirements for ISSR molecular markers and other assays.
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Comparison of DNA Extraction Methods of Asparagus

doi: 10.15886/j.cnki.rdswxb.2011.02.014
  • 1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101;
  • 2. College of Agriculture, Hainan University, Haikou 570228, China
  • Received Date: 2011-04-08

Abstract: In order to find the best DNA extraction method of asparagus,improved SDS,CTAB and kit method were used to extract DNA from Asparagus young leaves and the DNA quality also was tested by UV spectrophotometer,agarose gel electrophoresis and PCR detection.The results showed that the quality of the DNA from the three DNA extraction methods fill the requirements for ISSR molecular markers and other assays.

GAO Jianming, DAI Zhenzhen, JIANG Chengji, ZHANG Shiqing, CHEN Helong, ZHENG Jinlong, LIU Qiaolian, YI Kexian. Comparison of DNA Extraction Methods of Asparagus[J]. Journal of Tropical Biology, 2011, 2(2): 153-156. doi: 10.15886/j.cnki.rdswxb.2011.02.014
Citation: GAO Jianming, DAI Zhenzhen, JIANG Chengji, ZHANG Shiqing, CHEN Helong, ZHENG Jinlong, LIU Qiaolian, YI Kexian. Comparison of DNA Extraction Methods of Asparagus[J]. Journal of Tropical Biology, 2011, 2(2): 153-156. doi: 10.15886/j.cnki.rdswxb.2011.02.014
shu

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