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Volume 2 Issue 2
Jun.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(2): 129-132

FENG Yaowen, WANG Ying, GAO Heqiong, ZHUANG Nansheng. Physical Location of MeEFⅠfrom Manihot esculenta crantz by in situ PCR[J]. Journal of Tropical Biology, 2011, 2(2): 129-132. doi: 10.15886/j.cnki.rdswxb.2011.02.009
Citation: FENG Yaowen, WANG Ying, GAO Heqiong, ZHUANG Nansheng. Physical Location of MeEFⅠfrom Manihot esculenta crantz by in situ PCR[J]. Journal of Tropical Biology, 2011, 2(2): 129-132. doi: 10.15886/j.cnki.rdswxb.2011.02.009
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Physical Location of MeEFⅠfrom Manihot esculenta crantz by in situ PCR

doi: 10.15886/j.cnki.rdswxb.2011.02.009

  • 1. College of Agriculture, Hainan University, Haikou 570228, China;

  • 2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, China

  • Received Date: 2011-04-29
  • In our report,the chromosome was prepared using root tips of cassava variety SC6,the specific primers of MeEFⅠwere designed accoding to the complete cDNA sequence in GenBank,and in situ PCR was used to locate MeEFⅠin Manihot esculenta crantz genome.The results indicated that one or two signals were detected by in situ PCR on all phases of cell division;MEEFⅠgene located on the short arm of chromosome 10;the percent distances of the gene from centromere is 35.11.
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Physical Location of MeEFⅠfrom Manihot esculenta crantz by in situ PCR

doi: 10.15886/j.cnki.rdswxb.2011.02.009
  • 1. College of Agriculture, Hainan University, Haikou 570228, China;
  • 2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, China
  • Received Date: 2011-04-29

Abstract: In our report,the chromosome was prepared using root tips of cassava variety SC6,the specific primers of MeEFⅠwere designed accoding to the complete cDNA sequence in GenBank,and in situ PCR was used to locate MeEFⅠin Manihot esculenta crantz genome.The results indicated that one or two signals were detected by in situ PCR on all phases of cell division;MEEFⅠgene located on the short arm of chromosome 10;the percent distances of the gene from centromere is 35.11.

FENG Yaowen, WANG Ying, GAO Heqiong, ZHUANG Nansheng. Physical Location of MeEFⅠfrom Manihot esculenta crantz by in situ PCR[J]. Journal of Tropical Biology, 2011, 2(2): 129-132. doi: 10.15886/j.cnki.rdswxb.2011.02.009
Citation: FENG Yaowen, WANG Ying, GAO Heqiong, ZHUANG Nansheng. Physical Location of MeEFⅠfrom Manihot esculenta crantz by in situ PCR[J]. Journal of Tropical Biology, 2011, 2(2): 129-132. doi: 10.15886/j.cnki.rdswxb.2011.02.009
shu

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