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Volume 2 Issue 2
Jun.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(2): 101-106

WU Yuanli, YANG Benpeng, CAI Wenwei, ZHANG Shuzhen. Determination of Froctooligosaccharides in Transgenic Sugarcane with 1-SST using High Performance Liquid Chromatography（HPLC）[J]. Journal of Tropical Biology, 2011, 2(2): 101-106. doi: 10.15886/j.cnki.rdswxb.2011.02.008
Citation: WU Yuanli, YANG Benpeng, CAI Wenwei, ZHANG Shuzhen. Determination of Froctooligosaccharides in Transgenic Sugarcane with 1-SST using High Performance Liquid Chromatography（HPLC）[J]. Journal of Tropical Biology, 2011, 2(2): 101-106. doi: 10.15886/j.cnki.rdswxb.2011.02.008
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Determination of Froctooligosaccharides in Transgenic Sugarcane with 1-SST using High Performance Liquid Chromatography（HPLC）

doi: 10.15886/j.cnki.rdswxb.2011.02.008

  • 1. College of Agriculture, Hainan University, Danzhou 571737, China;

  • 2. Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101, China

  • Received Date: 2010-04-21
  • In order to determine varieties and contents of froctooligosaccharides in transgenic sugarcane transformed with 1-SST.Two saccharides determination methods using HPLC were established,one for determining the fructose,glucose and sucrose,the other for determining three Froctooligosaccharides（GF2,GF3 and GF4）.Saccharides of sugarcane were extracted with water,using a mobile phase composed of acetonitrile and water（87/13 V/V;75/25 V/V） at a flow rate of 1.0 mL·min-1 for separating,Hydersir NH2 column（4.6 mm×250 mm,5 μm） temperature was 30 ℃,the differential refraction detector was used to detect samples.The results indicated that the linear ranges of the six saccharides were 0.625—50 mg·mL-1,with a correlation coefficient exceeding 0.999 3.kestose GF2 in sugarcane transformed with the 1-SST gene were detected.Our methods can be used for accurate qualitative and quantitative determination of fructose,glucose and sucrose in sugarcane.
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Determination of Froctooligosaccharides in Transgenic Sugarcane with 1-SST using High Performance Liquid Chromatography（HPLC）

doi: 10.15886/j.cnki.rdswxb.2011.02.008
  • 1. College of Agriculture, Hainan University, Danzhou 571737, China;
  • 2. Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101, China
  • Received Date: 2010-04-21

Abstract: In order to determine varieties and contents of froctooligosaccharides in transgenic sugarcane transformed with 1-SST.Two saccharides determination methods using HPLC were established,one for determining the fructose,glucose and sucrose,the other for determining three Froctooligosaccharides（GF2,GF3 and GF4）.Saccharides of sugarcane were extracted with water,using a mobile phase composed of acetonitrile and water（87/13 V/V;75/25 V/V） at a flow rate of 1.0 mL·min-1 for separating,Hydersir NH2 column（4.6 mm×250 mm,5 μm） temperature was 30 ℃,the differential refraction detector was used to detect samples.The results indicated that the linear ranges of the six saccharides were 0.625—50 mg·mL-1,with a correlation coefficient exceeding 0.999 3.kestose GF2 in sugarcane transformed with the 1-SST gene were detected.Our methods can be used for accurate qualitative and quantitative determination of fructose,glucose and sucrose in sugarcane.

WU Yuanli, YANG Benpeng, CAI Wenwei, ZHANG Shuzhen. Determination of Froctooligosaccharides in Transgenic Sugarcane with 1-SST using High Performance Liquid Chromatography（HPLC）[J]. Journal of Tropical Biology, 2011, 2(2): 101-106. doi: 10.15886/j.cnki.rdswxb.2011.02.008
Citation: WU Yuanli, YANG Benpeng, CAI Wenwei, ZHANG Shuzhen. Determination of Froctooligosaccharides in Transgenic Sugarcane with 1-SST using High Performance Liquid Chromatography（HPLC）[J]. Journal of Tropical Biology, 2011, 2(2): 101-106. doi: 10.15886/j.cnki.rdswxb.2011.02.008
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