Fast Construction of dsRNA Prokaryotic Expression Vector of Papaya Ringspot Virus CP Gene

CHEN Yao; SHEN Wentao; YAN Pu; LI Xiaoying; ZHOU Peng

doi:10.15886/j.cnki.rdswxb.2011.02.006

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Volume 2 Issue 2

Jun. 2011

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Article Navigation > Journal of Tropical Biology > 2011 > 2(2): 113-116

CHEN Yao, SHEN Wentao, YAN Pu, LI Xiaoying, ZHOU Peng. Fast Construction of dsRNA Prokaryotic Expression Vector of Papaya Ringspot Virus CP Gene[J]. Journal of Tropical Biology, 2011, 2(2): 113-116. doi: 10.15886/j.cnki.rdswxb.2011.02.006

Citation:

CHEN Yao, SHEN Wentao, YAN Pu, LI Xiaoying, ZHOU Peng. Fast Construction of dsRNA Prokaryotic Expression Vector of Papaya Ringspot Virus CP Gene[J]. Journal of Tropical Biology, 2011, 2(2): 113-116. doi: 10.15886/j.cnki.rdswxb.2011.02.006

Fast Construction of dsRNA Prokaryotic Expression Vector of Papaya Ringspot Virus CP Gene

doi: 10.15886/j.cnki.rdswxb.2011.02.006

CHEN Yao^1,2,
SHEN Wentao²,
YAN Pu²,
LI Xiaoying²,
ZHOU Peng^{1,2
,
,}

1. College of Agriculture, Hainan University, Haikou 570228, China;
2. Key Biotechnology Laboratory for Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Analysis & testing center, CATAS, Haikou 571101, China

Received Date: 2011-06-27

Abstract

In the paper,a method for constructing dsRNA prokaryotic expression vector was introduced.The conserved region of PRSV-CP gene,which is 278 bp of 3'-end region,was used as the base,three hairpin structures of different lengths（861 bp,432 bp,279 bp） were cloned and constructed through one-step,zero-background ligation-independent cloning（OZ-LIC） method,and which were successively cloned into pSP73 prokaryotic expression vector after being digested with XhoⅠand ClaⅠ.Finally three recombinants of dsRNA prokaryotic expression plasmid of PRSV-CP gene were obtained.
- PRSV-CP,
- dsRNA,
- RNA silencing,
- OZ-LIC method,
- prokaryotic expression vector
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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