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Volume 1 Issue 3
Sep.  2010
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Article Navigation >  Journal of Tropical Biology  > 2010  >  1(3): 224-227

FENG Jiancheng, NIU Cheng, XING Xu, WANG Chao. Optimization on the Preparation of the Competent Cells of Escherichia coli HB101[J]. Journal of Tropical Biology, 2010, 1(3): 224-227. doi: 10.15886/j.cnki.rdswxb.2010.03.003
Citation: FENG Jiancheng, NIU Cheng, XING Xu, WANG Chao. Optimization on the Preparation of the Competent Cells of Escherichia coli HB101[J]. Journal of Tropical Biology, 2010, 1(3): 224-227. doi: 10.15886/j.cnki.rdswxb.2010.03.003
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Optimization on the Preparation of the Competent Cells of Escherichia coli HB101

doi: 10.15886/j.cnki.rdswxb.2010.03.003

  • College of Materials and Chemical Engineering, Hainan Universityv Haikou 570228, China;

  • Received Date: 2010-05-22
  • The strain source,the growth phrase of the cell and the storage condition were optimized for preparing the competent cells of Escherichia coli HB101. The results revealed that the highest transformation ability could be obtained when the source of the strain was streaked from strains stored under-70 ℃,cells were harvested when OD600 were 0.3—0.4,the storage condition of competent cells was under 4 ℃ for 1 d and-70 ℃ for 1 month,and the highest transformation efficiency attained 1.18×107 cfu·g-1 DNA.
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Optimization on the Preparation of the Competent Cells of Escherichia coli HB101

doi: 10.15886/j.cnki.rdswxb.2010.03.003
  • College of Materials and Chemical Engineering, Hainan Universityv Haikou 570228, China;
  • Received Date: 2010-05-22

Abstract: The strain source,the growth phrase of the cell and the storage condition were optimized for preparing the competent cells of Escherichia coli HB101. The results revealed that the highest transformation ability could be obtained when the source of the strain was streaked from strains stored under-70 ℃,cells were harvested when OD600 were 0.3—0.4,the storage condition of competent cells was under 4 ℃ for 1 d and-70 ℃ for 1 month,and the highest transformation efficiency attained 1.18×107 cfu·g-1 DNA.

FENG Jiancheng, NIU Cheng, XING Xu, WANG Chao. Optimization on the Preparation of the Competent Cells of Escherichia coli HB101[J]. Journal of Tropical Biology, 2010, 1(3): 224-227. doi: 10.15886/j.cnki.rdswxb.2010.03.003
Citation: FENG Jiancheng, NIU Cheng, XING Xu, WANG Chao. Optimization on the Preparation of the Competent Cells of Escherichia coli HB101[J]. Journal of Tropical Biology, 2010, 1(3): 224-227. doi: 10.15886/j.cnki.rdswxb.2010.03.003
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