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Volume 1 Issue 1
Mar.  2010
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Article Navigation >  Journal of Tropical Biology  > 2010  >  1(1): 55-58

LI Ze-you, SHEN Wen-tao, YAN Pu, LI Ya-li, ZHOU Peng. Cloning and Sequence Analysis of CYP79A2 Gene Fragment in Papaya[J]. Journal of Tropical Biology, 2010, 1(1): 55-58. doi: 10.15886/j.cnki.rdswxb.2010.01.015
Citation: LI Ze-you, SHEN Wen-tao, YAN Pu, LI Ya-li, ZHOU Peng. Cloning and Sequence Analysis of CYP79A2 Gene Fragment in Papaya[J]. Journal of Tropical Biology, 2010, 1(1): 55-58. doi: 10.15886/j.cnki.rdswxb.2010.01.015
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Cloning and Sequence Analysis of CYP79A2 Gene Fragment in Papaya

doi: 10.15886/j.cnki.rdswxb.2010.01.015

  • 1. College of Agriculture, Hainan University, Haikou 570228, China;

  • 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;

  • 3 Hainan Medical College, Haikou 571101, China

  • Received Date: 2009-03-25
  • L-phenylalanine N-hydroxylase is the first enzyme which catalyze the biosynthesis of benzylisothiocyanate （BITC） in plant. According to the sequences of CYP79A2 gene of Arabidopsis thaliana （NM120608） and Brassica rapa（EU877074）from GenBank and combining with the genome draft of the transgenic tropical fruit tree papaya,specific primers were designed and RT-PCR was used to amplify partial sequences of CYP79A2 gene from papaya young leaves. The sequence includes 923bp,which named CPCYP79A2. Bioinformatics analysis results showed that the frgment is the partial sequences of CYP79A2 gene of papaya. The data set a strong basis for the study of the biosynthetic regulation of BITC in papaya.
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Cloning and Sequence Analysis of CYP79A2 Gene Fragment in Papaya

doi: 10.15886/j.cnki.rdswxb.2010.01.015
  • 1. College of Agriculture, Hainan University, Haikou 570228, China;
  • 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
  • 3 Hainan Medical College, Haikou 571101, China
  • Received Date: 2009-03-25

Abstract: L-phenylalanine N-hydroxylase is the first enzyme which catalyze the biosynthesis of benzylisothiocyanate （BITC） in plant. According to the sequences of CYP79A2 gene of Arabidopsis thaliana （NM120608） and Brassica rapa（EU877074）from GenBank and combining with the genome draft of the transgenic tropical fruit tree papaya,specific primers were designed and RT-PCR was used to amplify partial sequences of CYP79A2 gene from papaya young leaves. The sequence includes 923bp,which named CPCYP79A2. Bioinformatics analysis results showed that the frgment is the partial sequences of CYP79A2 gene of papaya. The data set a strong basis for the study of the biosynthetic regulation of BITC in papaya.

LI Ze-you, SHEN Wen-tao, YAN Pu, LI Ya-li, ZHOU Peng. Cloning and Sequence Analysis of CYP79A2 Gene Fragment in Papaya[J]. Journal of Tropical Biology, 2010, 1(1): 55-58. doi: 10.15886/j.cnki.rdswxb.2010.01.015
Citation: LI Ze-you, SHEN Wen-tao, YAN Pu, LI Ya-li, ZHOU Peng. Cloning and Sequence Analysis of CYP79A2 Gene Fragment in Papaya[J]. Journal of Tropical Biology, 2010, 1(1): 55-58. doi: 10.15886/j.cnki.rdswxb.2010.01.015
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