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Volume 1 Issue 1
Mar.  2010
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Article Navigation >  Journal of Tropical Biology  > 2010  >  1(1): 1-7

LIN Sheng, PAN Da-ren, ZHOU Yi-fei, CHEN Guan-shui, ZHANG Xu-zhang, WU Zi-feng. Cloning of SoSgt1 from Chewing Cane and Construction of Antisense Vector[J]. Journal of Tropical Biology, 2010, 1(1): 1-7. doi: 10.15886/j.cnki.rdswxb.2010.01.010
Citation: LIN Sheng, PAN Da-ren, ZHOU Yi-fei, CHEN Guan-shui, ZHANG Xu-zhang, WU Zi-feng. Cloning of SoSgt1 from Chewing Cane and Construction of Antisense Vector[J]. Journal of Tropical Biology, 2010, 1(1): 1-7. doi: 10.15886/j.cnki.rdswxb.2010.01.010
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Cloning of SoSgt1 from Chewing Cane and Construction of Antisense Vector

doi: 10.15886/j.cnki.rdswxb.2010.01.010

  • 1. Key Laboratory of Crop Molecular and Cell Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;

  • 2. Key Laboratory of Ministry of Education of Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China

  • Received Date: 2009-10-10
  • Based on SGS conservative amino acid region of Sgt1 gene from different crops,degenerate primers were designed and RT-PCR was used to amplify SoSgt1 cDNA fragment of chewing cane. The results indicated thatSoSgt1 full length cDNA from chewing cane includes 1438 bp,362 amino acids. Protein Blast from NCBI database was used to analysis its amino acid sequence,and the results showed that the SoSgt1 protein have three SGT1 typical conserved domains eg. TPR,CS and SGS,and the similarity among SGT1 of other crops were very high. The expression levels ofSoSgt1 increased after inoculating with Gibberella fujikuroi. Based on SoSgt1 cDNA ,two restriction sites were designed and antisense expression vector pCAM-SGT were constructed,in which plant eukaryotic expression vector,pCAMBIA1301,were used as backbone.
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Cloning of SoSgt1 from Chewing Cane and Construction of Antisense Vector

doi: 10.15886/j.cnki.rdswxb.2010.01.010
  • 1. Key Laboratory of Crop Molecular and Cell Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
  • 2. Key Laboratory of Ministry of Education of Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received Date: 2009-10-10

Abstract: Based on SGS conservative amino acid region of Sgt1 gene from different crops,degenerate primers were designed and RT-PCR was used to amplify SoSgt1 cDNA fragment of chewing cane. The results indicated thatSoSgt1 full length cDNA from chewing cane includes 1438 bp,362 amino acids. Protein Blast from NCBI database was used to analysis its amino acid sequence,and the results showed that the SoSgt1 protein have three SGT1 typical conserved domains eg. TPR,CS and SGS,and the similarity among SGT1 of other crops were very high. The expression levels ofSoSgt1 increased after inoculating with Gibberella fujikuroi. Based on SoSgt1 cDNA ,two restriction sites were designed and antisense expression vector pCAM-SGT were constructed,in which plant eukaryotic expression vector,pCAMBIA1301,were used as backbone.

LIN Sheng, PAN Da-ren, ZHOU Yi-fei, CHEN Guan-shui, ZHANG Xu-zhang, WU Zi-feng. Cloning of SoSgt1 from Chewing Cane and Construction of Antisense Vector[J]. Journal of Tropical Biology, 2010, 1(1): 1-7. doi: 10.15886/j.cnki.rdswxb.2010.01.010
Citation: LIN Sheng, PAN Da-ren, ZHOU Yi-fei, CHEN Guan-shui, ZHANG Xu-zhang, WU Zi-feng. Cloning of SoSgt1 from Chewing Cane and Construction of Antisense Vector[J]. Journal of Tropical Biology, 2010, 1(1): 1-7. doi: 10.15886/j.cnki.rdswxb.2010.01.010
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