Construction of a lentiviral expression system for feline Tetranectin

YANG Zehao; HU Jiayi; ZHAO Yuanjie; LIU Mengmeng; CHEN Yu

doi:10.15886/j.cnki.rdswxb.20240155

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Tetranectin is a plasminogen-binding protein with the ability to bind calcium ions and heparin. To establish a stable cell line expressing feline tetranectin and provide experimental materials for subsequent investigations into its association with feline cardiovascular diseases, we constructed a recombinant lentiviral plasmid overexpressing the feline tetranectin gene. A HEK293T cell line overexpressing feline tetranectin was obtained through lentiviral infection, and protein expression was verified by Western blotting. Sequencing of the lentiviral plasmid confirmed that the coding sequence of the feline tetranectin gene was consistent with the predicted sequence in the NCBI database. The overexpression cell line was successfully established after puromycin selection. The recombinant feline tetranectin protein was secreted into the cell supernatant in a soluble form with a molecular weight of approximately 25 kDa and a FLAG tag.

Construction of a lentiviral expression system for feline Tetranectin

School of Tropical Agriculture and Forestry, Hainan university, Danzhou, Hainan 571737, China

Received Date: 2024-10-13

Rev Recd Date: 2024-11-20

Publish Date: 2025-03-15

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Abstract: Tetranectin is a plasminogen-binding protein with the ability to bind calcium ions and heparin. To establish a stable cell line expressing feline tetranectin and provide experimental materials for subsequent investigations into its association with feline cardiovascular diseases, we constructed a recombinant lentiviral plasmid overexpressing the feline tetranectin gene. A HEK293T cell line overexpressing feline tetranectin was obtained through lentiviral infection, and protein expression was verified by Western blotting. Sequencing of the lentiviral plasmid confirmed that the coding sequence of the feline tetranectin gene was consistent with the predicted sequence in the NCBI database. The overexpression cell line was successfully established after puromycin selection. The recombinant feline tetranectin protein was secreted into the cell supernatant in a soluble form with a molecular weight of approximately 25 kDa and a FLAG tag.

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Construction of a lentiviral expression system for feline Tetranectin

doi: 10.15886/j.cnki.rdswxb.20240155

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