YANG Min, HUANG Jing, CAO Yujie, LI Dongdong, ZHENG Yusheng. Identification and screening of differentially expressed genes in male and female Salacca zalacca using transcriptomic analysis[J]. Journal of Tropical Biology, 2025, 16(1): 13-20. doi: 10.15886/j.cnki.rdswxb.20240110
| Citation:
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YANG Min, HUANG Jing, CAO Yujie, LI Dongdong, ZHENG Yusheng. Identification and screening of differentially expressed genes in male and female Salacca zalacca using transcriptomic analysis[J]. Journal of Tropical Biology, 2025, 16(1): 13-20. doi: 10.15886/j.cnki.rdswxb.20240110
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Identification and screening of differentially expressed genes in male and female Salacca zalacca using transcriptomic analysis
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1. School of Life and Health Sciences, Hainan University, Haikou, Hainan 570228, China;
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2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
- Received Date: 2024-08-19
- Rev Recd Date:
2024-10-15
- Publish Date:
2025-03-15
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Abstract
Salacca zalacca（Arecaceae: Salaccinae） is a palm species characteristic of tropical regions, known for its dioecious nature with distinct male and female individuals. Early sex determination is crucial for optimizing the ratio of male to female seedlings in S. zalacca plantations. An attempt was made to obtain molecular markers for early sex differentiation. Leaves from three female and three male S. zalacca trees from Hainan Province were collected for transcriptome sequencing analysis, and 19 differentially expressed genes（DEGs） showing significant variations between male and female genotypes were identified. Further validation using an expanded cohort of seven female and seven male S. zalacca individuals confirmed that the Unigene0079610（GOLS1） gene exhibits stable differential expression patterns between the sexes. Consequently, the GOLS1 gene can serve as a robust molecular marker for sex identification in S. zalacca. Molecular signatures relevant to sex determination in S.zalacca were delineated through large-scale transcriptome screening, based on which a rapid detection protocol for the GOLS1 gene was established. These results provide a viable technological approach for the sex identification of S. zalacca nursery stock and offer a foundational framework for sex determination methods in other horticultural crops.
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