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Volume 16 Issue 1
Mar.  2025
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Article Navigation >  Journal of Tropical Biology  > 2025  >  16(1): 107-114

WU Jinjun, LI Zengping, ZHANG Yu, LIU Zhiyu. Identification and biological characteristics of an Amauroderma pathogen causing stem rot on Acacia mangium[J]. Journal of Tropical Biology, 2025, 16(1): 107-114. doi: 10.15886/j.cnki.rdswxb.20240078
Citation: WU Jinjun, LI Zengping, ZHANG Yu, LIU Zhiyu. Identification and biological characteristics of an Amauroderma pathogen causing stem rot on Acacia mangium[J]. Journal of Tropical Biology, 2025, 16(1): 107-114. doi: 10.15886/j.cnki.rdswxb.20240078
shu

Identification and biological characteristics of an Amauroderma pathogen causing stem rot on Acacia mangium

doi: 10.15886/j.cnki.rdswxb.20240078

  • School of Tropical Agriculture and Forestry, Hainan University, Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, Danzhou, Hainan 571737, China

  • Received Date: 2024-05-07
  • Rev Recd Date: 2024-07-09
  • Publish Date: 2025-03-15
  • An attempt was made to identify pathogens causing stem rot of Acacia mangium in Hainan and their biological characteristics to provide scientific theoretical basis for the occurrence and prevention of stem rot of A.mangium. The pathogens causing stem rot of A. mangium in Hainan were isolated and cultured by conventional tissue isolation method, and the representative strain HNBSMZXS20211011001 was obtained after purification.The pathogenicity of the isolated strain was tested by using Koch’s method and induced basidiocarp in the field.The results were consistent with the symptoms of plant disease in the field. The verification of Koch’s method was hence completed. According to the morphological characteristics of basidiocarps and basidiospores and molecular phylogenetic analysis of ITS, SSU, LSU and TEF1-α sequences, the Amauroderma pathogen causing stem rot of A. mangium in Hainan was identified as Amauroderma subresinosum. The colony of the pathogen cultured in PDA medium at 28 ℃ in darkness was round. At the early stage of growth, the colony was white and dense in the center, sparse and transparent on the edge, and it was light yellow in the late stage of growth. The biological characteristics of the strain showed that the pathogen had an optimum growth when cultured on PDA medium at 28 ℃ in darkness and pH5.
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Identification and biological characteristics of an Amauroderma pathogen causing stem rot on Acacia mangium

doi: 10.15886/j.cnki.rdswxb.20240078
  • School of Tropical Agriculture and Forestry, Hainan University, Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, Danzhou, Hainan 571737, China
  • Received Date: 2024-05-07
  • Rev Recd Date: 2024-07-09
  • Publish Date: 2025-03-15

Abstract: An attempt was made to identify pathogens causing stem rot of Acacia mangium in Hainan and their biological characteristics to provide scientific theoretical basis for the occurrence and prevention of stem rot of A.mangium. The pathogens causing stem rot of A. mangium in Hainan were isolated and cultured by conventional tissue isolation method, and the representative strain HNBSMZXS20211011001 was obtained after purification.The pathogenicity of the isolated strain was tested by using Koch’s method and induced basidiocarp in the field.The results were consistent with the symptoms of plant disease in the field. The verification of Koch’s method was hence completed. According to the morphological characteristics of basidiocarps and basidiospores and molecular phylogenetic analysis of ITS, SSU, LSU and TEF1-α sequences, the Amauroderma pathogen causing stem rot of A. mangium in Hainan was identified as Amauroderma subresinosum. The colony of the pathogen cultured in PDA medium at 28 ℃ in darkness was round. At the early stage of growth, the colony was white and dense in the center, sparse and transparent on the edge, and it was light yellow in the late stage of growth. The biological characteristics of the strain showed that the pathogen had an optimum growth when cultured on PDA medium at 28 ℃ in darkness and pH5.

WU Jinjun, LI Zengping, ZHANG Yu, LIU Zhiyu. Identification and biological characteristics of an Amauroderma pathogen causing stem rot on Acacia mangium[J]. Journal of Tropical Biology, 2025, 16(1): 107-114. doi: 10.15886/j.cnki.rdswxb.20240078
Citation: WU Jinjun, LI Zengping, ZHANG Yu, LIU Zhiyu. Identification and biological characteristics of an Amauroderma pathogen causing stem rot on Acacia mangium[J]. Journal of Tropical Biology, 2025, 16(1): 107-114. doi: 10.15886/j.cnki.rdswxb.20240078
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