Cloning of soybean <i>GmACBP6</i> gene and functional Validation of salinity tolerance during germination

WU Guanji; ZHOU Xunjun; WU Feifei; ZHANG Yinhe; HUANG Yinghua; LI Yaxin; GAO Hongtao; LI Haiyan

doi:10.15886/j.cnki.rdswxb.20240060

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In order to determine whether soybean（Glycine max） acyl-Coenzyme A binding protein（GmACBP6）has saline-tolerant function, the expression of GmACBP6 during the germination period was analyzed by using the real-time PCR, and analysis showed that GmACBP6 responded to saline-alkali stress in the germination period of soybean. In this context the pYES2-GmACBP6 recombinant vector was constructed and transformed into the yeast strains, The growth of pYES2-GmACBP6 yeast strain and pYES2 yeast strain cultured in salt and saline stress mediums were observed. The results show that the pYES 2-GmACBP6 yeast strain grew significantly better under salt and salt stress than the pYES2 yeast vector. It is preliminarily determined that the soybean GmACBP6gene has the function of improving the salt and alkali tolerance of yeast cells. In this study the A. thaliana materials with GmACBP6 knock-in and overexpression were also produced, and their salinity-tolerant phenotype during the germination stage was identified. The results showed that the germination rate of Arabidopsis seeds with GmACBP6 overexpression was significantly higher than that of GmACBP6 knock-in, atacbp6 mutant or wild Arabidopsis thaliana under salt and salt-alkali stress, which further confirmed the salt-alkali tolerance of the GmACBP6 gene in the soybean.

Cloning of soybean GmACBP6 gene and functional Validation of salinity tolerance during germination

1. Schiool of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570288;

2. Nanfan College Sanya Nanfan Research Institute, Hainan University Sanya, 572024

Received Date: 2024-04-10

Rev Recd Date: 2024-04-26

Publish Date: 2024-07-25

Key words:

Abstract: In order to determine whether soybean（Glycine max） acyl-Coenzyme A binding protein（GmACBP6）has saline-tolerant function, the expression of GmACBP6 during the germination period was analyzed by using the real-time PCR, and analysis showed that GmACBP6 responded to saline-alkali stress in the germination period of soybean. In this context the pYES2-GmACBP6 recombinant vector was constructed and transformed into the yeast strains, The growth of pYES2-GmACBP6 yeast strain and pYES2 yeast strain cultured in salt and saline stress mediums were observed. The results show that the pYES 2-GmACBP6 yeast strain grew significantly better under salt and salt stress than the pYES2 yeast vector. It is preliminarily determined that the soybean GmACBP6gene has the function of improving the salt and alkali tolerance of yeast cells. In this study the A. thaliana materials with GmACBP6 knock-in and overexpression were also produced, and their salinity-tolerant phenotype during the germination stage was identified. The results showed that the germination rate of Arabidopsis seeds with GmACBP6 overexpression was significantly higher than that of GmACBP6 knock-in, atacbp6 mutant or wild Arabidopsis thaliana under salt and salt-alkali stress, which further confirmed the salt-alkali tolerance of the GmACBP6 gene in the soybean.

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[1]	GAO W, XIAO S, LI H Y, et al. Arabidopsis thaliana acylCoA-binding protein ACBP2 interacts with heavy-metalbinding farnesylated protein AtFP6[J]. The New Phytologist, 2009, 181(1):89-102.
[2]	CHEN Q F, XIAO S, QI W, et al. The Arabidopsis acbp1acbp2 double mutant lacking acyl-CoA-binding proteins ACBP1 and ACBP2 is embryo lethal[J]. The New Phytologist, 2010, 186(4):843-855.
[3]	XIAO S, GAO W, CHEN Q F, et al. Overexpression of Arabidopsis acyl-Co A binding protein ACBP3 promotes starvation-induced and age-dependent leaf senescence[J].The Plant Cell, 2010, 22(5):1463-1482.
[4]	DU Z Y, CHEN M X, CHEN Q F, et al. Overexpression of Arabidopsis acyl-CoA-binding protein ACBP2 enhances drought tolerance[J]. Plant, Cell&Environment, 2013,36(2):300-314.
[5]	HAMDAN M F, LUNG S C, GUO Z H, et al. Roles of acylCoA-binding proteins in plant reproduction[J]. Journal of Experimental Botany, 2022, 73(9):2918-2936.
[6]	DU Z Y, ARIAS T, MENG W, et al. Plant acyl-CoAbinding proteins:an emerging family involved in plant development and stress responses[J]. Progress in Lipid Research, 2016, 63:165-181.
[7]	秦朋飞.棉花酰基辅酶A结合蛋白(ACBP)家族基因的发掘及在非生物胁迫抗性中的功能鉴定[J].作物学报,2016,42(11):1577-1591.
[8]	周远远. ZmACBPs家族的分子鉴定、表达及功能分析[D].济南:济南大学, 2021.
[9]	CHANG Y. Genome-wide identification and characterization of ACBP gene family in Populus reveal salinity alkaliresponsive profiles[J]. Journal of Forestry Research,2023,34(2):481-496.
[10]	滕涛.中国大豆产业链价值提升策略研究[D].大连:大连海事大学,2010.
[11]	张旗,陶梦慧,李丹,等.盐碱胁迫对野生大豆种子萌发的影响[J].畜牧与饲料科学, 2022, 43(4):104-108.
[12]	ZHU J, LI W, ZHOU Y, et al. Molecular characterization,expression and functional analysis of acyl-CoA-binding protein gene family in maize(Zea mays)[J]. BMC Plant Biology, 2021, 21(1):94.
[13]	HURLOCK A K, ROSTON R L, WANG K, et al. Lipid trafficking in plant cells[J]. Traffic, 2014, 15(9):915-932.
[14]	LUNG S C, LAI S H, WANG H, et al. Oxylipin signaling in salt-stressed soybean is modulated by liganddependent interaction of ClassⅡacyl-CoA-binding proteins with lipoxygenase[J]. The Plant Cell, 2022, 34(3):1117-1143.
[15]	XIAO S, GAO W, CHEN Q F, et al. Overexpression of membrane-associated acyl-Co A-binding protein ACBP1enhances lead tolerance in Arabidopsis[J]. The Plant Journal:for Cell and Molecular Biology, 2008, 54(1):141-151.
[16]	DU Z Y, CHEN M X, CHEN Q F, et al. Arabidopsis acylCoA-binding protein ACBP1 participates in the regulation of seed germination and seedling development[J].The Plant Journal:for Cell and Molecular Biology, 2013,74(2):294-309.
[17]	XUE Y, XIAO S, KIM J, et al. Arabidopsis membraneassociated acyl-CoA-binding protein ACBP1 is involved in stem cuticle formation[J]. Journal of Experimental Botany, 2014, 65(18):5473-5483.
[18]	ZIMMERMANN P, HIRSCH-HOFFMANN M, HENNIG L, et al. GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox[J]. Plant Physiology, 2004,136(1):2621-2632.
[19]	AZNAR-MORENO J A, VENEGAS-CALERÓN M, DU Z Y, et al. Characterization of a small acyl-CoA-binding protein(ACBP)from Helianthus annuus L. and its binding affinities[J]. Plant Physiology and Biochemistry:PPB,2016, 102:141-150.
[20]	张引鹤,周永刚,高红桃,等.大豆(Glycine max)SUT4基因的克隆、表达及抗逆功能的鉴定[J].分子植物育种, 2022, 20(17):5578-5587.

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Cloning of soybean GmACBP6 gene and functional Validation of salinity tolerance during germination

doi: 10.15886/j.cnki.rdswxb.20240060

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Cloning of soybean GmACBP6 gene and functional Validation of salinity tolerance during germination

doi: 10.15886/j.cnki.rdswxb.20240060

1. Schiool of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570288;

2. Nanfan College Sanya Nanfan Research Institute, Hainan University Sanya, 572024

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Cloning of soybean GmACBP6 gene and functional Validation of salinity tolerance during germination

doi: 10.15886/j.cnki.rdswxb.20240060

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Related

Cloning of soybean GmACBP6 gene and functional Validation of salinity tolerance during germination

doi: 10.15886/j.cnki.rdswxb.20240060

1. Schiool of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570288; 2. Nanfan College Sanya Nanfan Research Institute, Hainan University Sanya, 572024

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1. Schiool of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570288;

2. Nanfan College Sanya Nanfan Research Institute, Hainan University Sanya, 572024