Comparative analysis of stomatal density and physiological indexes of cassava under drought stress

ZHANG Yuanlei; ZHANG Hongtu; XU Ziyin; LI Shuxia; YU Xiaoling; ZHAO Pingjuan; LI Wenbin; ZHANG Xiuchun; YU Xiaohui; RUAN Mengbin

doi:10.15886/j.cnki.rdswxb.20240056

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Volume 16 Issue 2

Apr. 2025

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Article Navigation > Journal of Tropical Biology > 2025 > 16(2): 163-171

ZHANG Yuanlei, ZHANG Hongtu, XU Ziyin, LI Shuxia, YU Xiaoling, ZHAO Pingjuan, LI Wenbin, ZHANG Xiuchun, YU Xiaohui, RUAN Mengbin. Comparative analysis of stomatal density and physiological indexes of cassava under drought stress[J]. Journal of Tropical Biology, 2025, 16(2): 163-171. doi: 10.15886/j.cnki.rdswxb.20240056

Citation:

ZHANG Yuanlei, ZHANG Hongtu, XU Ziyin, LI Shuxia, YU Xiaoling, ZHAO Pingjuan, LI Wenbin, ZHANG Xiuchun, YU Xiaohui, RUAN Mengbin. Comparative analysis of stomatal density and physiological indexes of cassava under drought stress[J]. Journal of Tropical Biology, 2025, 16(2): 163-171. doi: 10.15886/j.cnki.rdswxb.20240056

Comparative analysis of stomatal density and physiological indexes of cassava under drought stress

doi: 10.15886/j.cnki.rdswxb.20240056

1. School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228;
2. Hainan Key Laboratory of Tropical Agricultural Biological Resources Conservation and Utilization, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101;
3. Sanya Academy/National Key Laboratory of Tropical Crop Biological Breeding, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572025, China

Received Date: 2024-04-08
Rev Recd Date: 2024-05-09

Abstract

Cassava is an important tropical food crop with high light efficiency and drought tolerance. Stomata is the gateway through which water and air exchange between plant leaves and the external environment. Stomata density directly affect the drought resistance of plants. However, the physiological mechanism of stomata in the formation of drought tolerance in cassava is not clear. In order to analyze the physiological regulation mechanism of cassava under drought stress, the stomatal density of 93 cassava cultivars was statistically analyzed, the changes of stomatal density of different cassava cultivars under drought stress were compared, and the physiological and biochemical indexes of their leaves were measured to analyze the relationship between stomatal density and physiological indexes of different cassava cultivars and their drought resistance. The results showed that stomatal density of cassava cultivars under drought stress was not consistent. Overall, there was a weak negative correlation between stomatal density and drought resistance（correlation coefficient was -0.15）. The soluble sugar content and transpiration rate decreased significantly in 6 cassava cultivars（3 with low stomatal density and 3 with high stomatal density） under drought stress, but the change was not very consistent in both the high and low stomatal density cultivars. It was hence concluded that drought had a greater impact on cassava growth, and that cassava might respond to drought stress quickly through the synthesis of osmoregulatory substances and antioxidant enzymes, which further affect the differentiation and development of stomata.
- cassava,
- stomata,
- drought stress,
- physiological indicators

References

[1]	BROWN A L, CAVAGNARO T R, GLEADOW R, et al.Interactive effects of temperature and drought on cassava growth and toxicity:implications for food security?[J]. Global Change Biology, 2016, 22(10):3461-3473.
[2]	LI S, CUI Y, ZHOU Y, et al. The industrial applications of cassava:current status, opportunities and prospects[J]. Journal of the Science of Food and Agriculture, 2017,97(8):2282-2290.
[3]	AINSWORTH E A, LONG S P. 30 years of free-air carbon dioxide enrichment(FACE):what have we learned about future crop productivity and its potential for adaptation?[J]. Global Change Biology, 2021, 27(1):27-49.
[4]	UTSUMI Y, TANAKA M, MOROSAWA T, et al. Transcriptome analysis using a high-density oligomicroarray under drought stress in various genotypes of cassava:an important tropical crop[J]. DNA Research:an International Journal for Rapid Publication of Reports on Genes and Genomes, 2012, 19(4):335-345.
[5]	YE Z Q, WANG J M, WANG W J, et al. Effects of root phenotypic changes on the deep rooting of Populus euphratica seedlings under drought stresses[J]. PeerJ, 2019, 7:e6513.
[6]	LI X, SMITH R, CHOAT B, et al. Drought resistance of cotton(Gossypium hirsutum) is promoted by early stomatal closure and leaf shedding[J]. Functional Plant Biology:FPB, 2020, 47(2):91-98.
[7]	EL-SHARKAWY M A. Cassava biology and physiology[J]. Plant Molecular Biology, 2004, 56(4):481-501.
[8]	于晓玲,王淦,阮孟斌,等.水分胁迫对不同木薯品种叶片生理生化的影响[J]. 中国农学通报, 2012, 28(33):60-64.
[9]	HETHERINGTON A M, WOODWARD F I. The role of stomata in sensing and driving environmental change[J]. Nature, 2003, 424:901-908.
[10]	CAINE R S, HARRISON E L, SLOAN J, et al. The influences of stomatal size and density on rice abiotic stress resilience[J]. The New Phytologist, 2023, 237(6):2180-2195.
[11]	MARRON N, DILLEN S Y, CEULEMANS R. Evaluation of leaf traits for indirect selection of high yielding poplar hybrids[J]. Environmental and Experimental Botany,2007, 61(2):103-116.
[12]	单提波,姚文钧,徐正进,等.不同气孔密度水稻剑叶光合特性及蔗糖代谢研究[J]. 中国水稻科学, 2015,29(6):648-652.
[13]	陈璐,陈默,吴志鹏,等.水分胁迫下木薯(NZ199)苗期生理响应分析[J]. 江西农业大学学报, 2023, 45(4):855-865.
[14]	WANG B, GUO X, ZHAO P, et al. Molecular diversity analysis, drought related marker-traits association mapping and discovery of excellent alleles for 100-day old plants by EST-SSRs in cassava germplasms(Manihot esculenta Cranz)[J]. PLoS One, 2017, 12(5):e0177456.
[15]	李俊,杨玉皎,王文丽,等. UV-B辐射增强对马铃薯叶片结构及光合参数的影响[J]. 生态学报, 2017, 37(16):5368-5381.
[16]	季蕾蕾,木泰华,孙红男.不同干燥方式对甘薯叶片水分迁移、微观结构、色泽及复水性能影响的比较[J]. 食品科学, 2020, 41(11):90-96.
[17]
[18]	王巧艳.玉米副卫细胞中H₂O₂与气孔开关的关系及其来源探究[D]. 杨凌:西北农林科技大学, 2016.
[19]	BESTETTI S, MEDRAÑO-FERNANDEZ I, GALLI M, et al. A persulfidation-based mechanism controls aquaporin-8conductance[J]. Science Advances, 2018, 4(5):eaar5770.
[20]	YU H, CHEN X, HONG Y Y, et al. Activated expression of an Arabidopsis HD-START protein confers drought tolerance with improved root system and reduced stomatal density[J]. The Plant Cell, 2008, 20(4):1134-1151.
[21]	YOO C Y, PENCE H E, JIN J B, et al. The Arabidopsis GTL1 transcription factor regulates water use efficiency and drought tolerance by modulating stomatal density via transrepression of SDD1[J]. The Plant Cell, 2012, 22(12):4128-4141.
[22]	FRANKS P J, DOHENY-ADAMS T W, BRITTONHARPER Z J, et al. Increasing water-use efficiency directly through genetic manipulation of stomatal density[J]. The New Phytologist, 2015, 207(1):188-195.
[23]	HEPWORTH C, DOHENY-ADAMS T, HUNT L, et al.Manipulating stomatal density enhances drought tolerance without deleterious effect on nutrient uptake[J]. The New Phytologist, 2015, 208(2):336-341.
[24]	MENG L S, YAO S Q. Transcription co-activator Arabidopsis ANGUSTIFOLIA3(AN3) regulates water-use efficiency and drought tolerance by modulating stomatal density and improving root architecture by the transrepression of YODA(YDA)[J]. Plant Biotechnology Journal,2015, 13(7):893-902.
[25]	KUMAR S, TRIPATHI S, SINGH S P, et al. Rice breeding for yield under drought has selected for longer flag leaves and lower stomatal density[J]. Journal of Experimental Botany, 2021, 72(13):4981-4992.
[26]	XIN H, LI Q, WANG S, et al. Saussurea involucrata PIP2;4 improves growth and drought tolerance in Nicotiana tabacum by increasing stomatal density and sensitivity[J]. Plant Science:an International Journal of Experimental Plant Biology, 2023, 326:111526.
[27]	何士敏,汪建华,秦家顺.几种沙棘叶片组织结构特点和抗旱性比较[J]. 林业科技开发, 2009, 23(1):16-19.
[28]	XU Z, ZHOU G. Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass[J]. Journal of Experimental Botany, 2008, 59(12):3317-3325.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Comparative analysis of stomatal density and physiological indexes of cassava under drought stress

1. School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228;

2. Hainan Key Laboratory of Tropical Agricultural Biological Resources Conservation and Utilization, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101;

3. Sanya Academy/National Key Laboratory of Tropical Crop Biological Breeding, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572025, China

Received Date: 2024-04-08

Rev Recd Date: 2024-05-09

Key words:

Abstract: Cassava is an important tropical food crop with high light efficiency and drought tolerance. Stomata is the gateway through which water and air exchange between plant leaves and the external environment. Stomata density directly affect the drought resistance of plants. However, the physiological mechanism of stomata in the formation of drought tolerance in cassava is not clear. In order to analyze the physiological regulation mechanism of cassava under drought stress, the stomatal density of 93 cassava cultivars was statistically analyzed, the changes of stomatal density of different cassava cultivars under drought stress were compared, and the physiological and biochemical indexes of their leaves were measured to analyze the relationship between stomatal density and physiological indexes of different cassava cultivars and their drought resistance. The results showed that stomatal density of cassava cultivars under drought stress was not consistent. Overall, there was a weak negative correlation between stomatal density and drought resistance（correlation coefficient was -0.15）. The soluble sugar content and transpiration rate decreased significantly in 6 cassava cultivars（3 with low stomatal density and 3 with high stomatal density） under drought stress, but the change was not very consistent in both the high and low stomatal density cultivars. It was hence concluded that drought had a greater impact on cassava growth, and that cassava might respond to drought stress quickly through the synthesis of osmoregulatory substances and antioxidant enzymes, which further affect the differentiation and development of stomata.

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Reference (28)

[1]	BROWN A L, CAVAGNARO T R, GLEADOW R, et al.Interactive effects of temperature and drought on cassava growth and toxicity:implications for food security?[J]. Global Change Biology, 2016, 22(10):3461-3473.
[2]	LI S, CUI Y, ZHOU Y, et al. The industrial applications of cassava:current status, opportunities and prospects[J]. Journal of the Science of Food and Agriculture, 2017,97(8):2282-2290.
[3]	AINSWORTH E A, LONG S P. 30 years of free-air carbon dioxide enrichment(FACE):what have we learned about future crop productivity and its potential for adaptation?[J]. Global Change Biology, 2021, 27(1):27-49.
[4]	UTSUMI Y, TANAKA M, MOROSAWA T, et al. Transcriptome analysis using a high-density oligomicroarray under drought stress in various genotypes of cassava:an important tropical crop[J]. DNA Research:an International Journal for Rapid Publication of Reports on Genes and Genomes, 2012, 19(4):335-345.
[5]	YE Z Q, WANG J M, WANG W J, et al. Effects of root phenotypic changes on the deep rooting of Populus euphratica seedlings under drought stresses[J]. PeerJ, 2019, 7:e6513.
[6]	LI X, SMITH R, CHOAT B, et al. Drought resistance of cotton(Gossypium hirsutum) is promoted by early stomatal closure and leaf shedding[J]. Functional Plant Biology:FPB, 2020, 47(2):91-98.
[7]	EL-SHARKAWY M A. Cassava biology and physiology[J]. Plant Molecular Biology, 2004, 56(4):481-501.
[8]	于晓玲,王淦,阮孟斌,等.水分胁迫对不同木薯品种叶片生理生化的影响[J]. 中国农学通报, 2012, 28(33):60-64.
[9]	HETHERINGTON A M, WOODWARD F I. The role of stomata in sensing and driving environmental change[J]. Nature, 2003, 424:901-908.
[10]	CAINE R S, HARRISON E L, SLOAN J, et al. The influences of stomatal size and density on rice abiotic stress resilience[J]. The New Phytologist, 2023, 237(6):2180-2195.
[11]	MARRON N, DILLEN S Y, CEULEMANS R. Evaluation of leaf traits for indirect selection of high yielding poplar hybrids[J]. Environmental and Experimental Botany,2007, 61(2):103-116.
[12]	单提波,姚文钧,徐正进,等.不同气孔密度水稻剑叶光合特性及蔗糖代谢研究[J]. 中国水稻科学, 2015,29(6):648-652.
[13]	陈璐,陈默,吴志鹏,等.水分胁迫下木薯(NZ199)苗期生理响应分析[J]. 江西农业大学学报, 2023, 45(4):855-865.
[14]	WANG B, GUO X, ZHAO P, et al. Molecular diversity analysis, drought related marker-traits association mapping and discovery of excellent alleles for 100-day old plants by EST-SSRs in cassava germplasms(Manihot esculenta Cranz)[J]. PLoS One, 2017, 12(5):e0177456.
[15]	李俊,杨玉皎,王文丽,等. UV-B辐射增强对马铃薯叶片结构及光合参数的影响[J]. 生态学报, 2017, 37(16):5368-5381.
[16]	季蕾蕾,木泰华,孙红男.不同干燥方式对甘薯叶片水分迁移、微观结构、色泽及复水性能影响的比较[J]. 食品科学, 2020, 41(11):90-96.
[17]
[18]	王巧艳.玉米副卫细胞中H₂O₂与气孔开关的关系及其来源探究[D]. 杨凌:西北农林科技大学, 2016.
[19]	BESTETTI S, MEDRAÑO-FERNANDEZ I, GALLI M, et al. A persulfidation-based mechanism controls aquaporin-8conductance[J]. Science Advances, 2018, 4(5):eaar5770.
[20]	YU H, CHEN X, HONG Y Y, et al. Activated expression of an Arabidopsis HD-START protein confers drought tolerance with improved root system and reduced stomatal density[J]. The Plant Cell, 2008, 20(4):1134-1151.
[21]	YOO C Y, PENCE H E, JIN J B, et al. The Arabidopsis GTL1 transcription factor regulates water use efficiency and drought tolerance by modulating stomatal density via transrepression of SDD1[J]. The Plant Cell, 2012, 22(12):4128-4141.
[22]	FRANKS P J, DOHENY-ADAMS T W, BRITTONHARPER Z J, et al. Increasing water-use efficiency directly through genetic manipulation of stomatal density[J]. The New Phytologist, 2015, 207(1):188-195.
[23]	HEPWORTH C, DOHENY-ADAMS T, HUNT L, et al.Manipulating stomatal density enhances drought tolerance without deleterious effect on nutrient uptake[J]. The New Phytologist, 2015, 208(2):336-341.
[24]	MENG L S, YAO S Q. Transcription co-activator Arabidopsis ANGUSTIFOLIA3(AN3) regulates water-use efficiency and drought tolerance by modulating stomatal density and improving root architecture by the transrepression of YODA(YDA)[J]. Plant Biotechnology Journal,2015, 13(7):893-902.
[25]	KUMAR S, TRIPATHI S, SINGH S P, et al. Rice breeding for yield under drought has selected for longer flag leaves and lower stomatal density[J]. Journal of Experimental Botany, 2021, 72(13):4981-4992.
[26]	XIN H, LI Q, WANG S, et al. Saussurea involucrata PIP2;4 improves growth and drought tolerance in Nicotiana tabacum by increasing stomatal density and sensitivity[J]. Plant Science:an International Journal of Experimental Plant Biology, 2023, 326:111526.
[27]	何士敏,汪建华,秦家顺.几种沙棘叶片组织结构特点和抗旱性比较[J]. 林业科技开发, 2009, 23(1):16-19.
[28]	XU Z, ZHOU G. Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass[J]. Journal of Experimental Botany, 2008, 59(12):3317-3325.

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Comparative analysis of stomatal density and physiological indexes of cassava under drought stress

doi: 10.15886/j.cnki.rdswxb.20240056

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

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