Analysis of starch accumulation in root tuber of cassava cultivars during different harvesting periods

ZHENG Yongqing; LI Kaimian; LI Bosong; MA Xudong; CHEN Songbi

doi:10.15886/j.cnki.rdswxb.20240051

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Volume 16 Issue 2

Apr. 2025

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Article Navigation > Journal of Tropical Biology > 2025 > 16(2): 172-180

ZHENG Yongqing, LI Kaimian, LI Bosong, MA Xudong, CHEN Songbi. Analysis of starch accumulation in root tuber of cassava cultivars during different harvesting periods[J]. Journal of Tropical Biology, 2025, 16(2): 172-180. doi: 10.15886/j.cnki.rdswxb.20240051

Citation:

ZHENG Yongqing, LI Kaimian, LI Bosong, MA Xudong, CHEN Songbi. Analysis of starch accumulation in root tuber of cassava cultivars during different harvesting periods[J]. Journal of Tropical Biology, 2025, 16(2): 172-180. doi: 10.15886/j.cnki.rdswxb.20240051

Analysis of starch accumulation in root tuber of cassava cultivars during different harvesting periods

doi: 10.15886/j.cnki.rdswxb.20240051

1. Guangzhou Experimental Station, CATAS, Guangzhou, Guangdong 510140;
2. Tropical Crops Genetic Resources Institute, CATAS, Haikou, Hainan 571737, China

Received Date: 2024-03-26
Rev Recd Date: 2024-10-29

Abstract

In order to analyze the law of starch accumulation in root tubers of cassava cultivars in different harvesting periods, 4 cassava cultivars ‘SC205’, ‘SC8’, ‘SC9’ and ‘SC6068’ were determined in terms of fresh weight and biomass of root tubers and ratio of amylose and amylopectin in starch after 6 to 10 months of planting,and the underlying mechanism was analyzed. The results showed that there were significant differences in fresh tuber weight in different cassava harvesting periods. There were significant differences in amylose ratio in different harvesting periods in the same cultivar, and the amylose ratio increased in the later growth period of cassava. The ribulose diphosphate carboxylase（Rubisco） content of high-yielding cultivars SC205 and SC8 was generally lower than that of low-yielding cultivars ‘SC6068’ and ‘SC9’ after 7 to 10 months of planting. At 7 to 10 months after planting, the content of PSⅡ oxygen releasing complex gradually increased in SC8, was relatively stable in SC205, and gradually decreased in SC9 and SC6068 at 9 to 10 months after planting. In October cassava production was the highest, and cultivars ‘SC205’, ‘SC9’ and ‘SC6068’ were the highest in the content of PSⅡoxygen releasing complex. The cultivars ‘SC205’, ‘SC8’,‘SC9’ and ‘SC6068’ showed an increasing trend in the content of sucrose phosphate synthetase（SPS） after October, whereas the activity of β-amylase increased from September to October, and decreased from November to December. The protein content of ascorbate peroxidase（APX） was relatively stable in ‘SC6068’ from September to December. The cultivars ‘SC205’, ‘SC8’ and ‘SC9’ showed an increasing trend in APX content from September to December. In conclusion, there were significant differences in yield and starch composition among different cultivars, the mechanism of which was closely related to leaf photosynthesis, antioxidant activity and root starch synthetase activity.
- cassava,
- yield,
- starch,
- photosynthesis

References

[1]	HOANG N H, LE THANH T, THEPBANDIT W, et al.Efficacy of chitosan nanoparticle loaded-salicylic acid and-silver on management of cassava leaf spot disease[J]. Polymers, 2022, 14(4):660.
[2]	苏必孟,刘子凡,黄洁,等.基于主成分分析的木薯抗旱栽培措施的综合评价[J]. 热带作物学报, 2017, 38(2):189-193.
[3]	CARVALHO L J C B, DE SOUZA C R B, DE CASCARDO J C M, et al. Identification and characterization of a novel cassava(Manihot esculenta Crantz)clone with high free sugar content and novel starch[J]. Plant Molecular Biology, 2004, 56(4):643-659.
[4]	WANG Y J, LU X H, ZHEN X H, et al. A transformation and genome editing system for cassava cultivar SC8[J]. Genes, 2022, 13(9):1650.
[5]	罗兴录.不同植物生长调节剂对木薯生长发育和淀粉积累影响的研究[J]. 中国农学通报, 2002, 18(3):30-33.
[6]	罗兴录,劳天源.木薯不同时期施肥对产量和淀粉积累影响研究[J]. 耕作与栽培, 2000,(03):8-9.
[7]	黄洁,林雄,李开绵,等.木薯施肥效应研究[J]. 广西热作科技, 2000(3):1-3.
[8]	罗兴录,岑忠用,谢和霞,等.不同木薯品种抗衰老生理与淀粉积累特性研究[J]. 作物学报, 2007, 33(6):1018-1024.
[9]	HE S, HAO X, WANG S, et al. Starch synthase II plays a crucial role in starch biosynthesis and the formation of multienzyme complexes in cassava storage roots[J]. Journal of Experimental Botany, 2022, 73(8):2540-2557.
[10]	SAITHONG T, RONGSIRIKUL O, KALAPANULAK S,et al. Starch biosynthesis in cassava:a genome-based pathway reconstruction and its exploitation in data integration[J]. BMC Systems Biology, 2013, 7:75.
[11]	HAN J, GU L, WEN J, et al. Inference of photosynthetic capacity parameters from chlorophyll a fluorescence is affected by redox state of PSII reaction centers[J]. Plant,Cell&Environment, 2022, 45(4):1298-1314.
[12]	ECKARDT N A. From the archives:Photosynthesis matters; PSII antenna size, photorespiration, and the evolution of C4 photosynthesis[J]. The Plant Cell, 2022, 34(4):1145-1146.
[13]	PANPETCH P, FIELD R A, LIMPASENI T. Cloning of the full-length isoamylase3 gene from cassava Manihot esculenta Crantz ‘KU50’ and its heterologous expression in E. coli[J]. Plant Physiology and Biochemistry:PPB,2018, 132:281-286.
[14]	UARROTA V G, MORESCO R, SCHMIDT E C, et al.The role of ascorbate peroxidase, guaiacol peroxidase,and polysaccharides in cassava(Manihot esculenta Crantz)roots under postharvest physiological deterioration[J]. Food Chemistry, 2016, 197(Pt A):737-746.
[15]	平吉成,单守明,刘晶,等.库源关系对草莓叶片光合磷酸化和Rubisco活性的影响[J]. 北方园艺, 2011(19):1-4.
[16]	SUGANAMI M, SUZUKI Y, TAZOE Y, et al. Cooverproducing Rubisco and Rubisco activase enhances photosynthesis in the optimal temperature range in rice[J]. Plant Physiology, 2021, 185(1):108-119.
[17]	黄洁,周建国,林世欣,等.对胶园间作木薯的思考及展望[J]. 江西农业学报, 2014, 26(1):64-68.
[18]	罗兴录,陈会鲜,杨鑫,等.木薯不同灌溉方式对产量和土壤理化性状影响研究[J]. 中国农学通报, 2014,30(18):151-155.
[19]	ŚLESAK I, MAZUR Z,ŚLESAK H. Genes encoding the photosystemⅡproteins are under purifying selection:an insight into the early evolution of oxygenic photosynthesis[J]. Photosynthesis Research, 2022, 153(3):163-175.
[20]	罗兴录,周贵靖,朱艳梅,等.药肥施用量对土壤养分和木薯产量的影响[J]. 广东农业科学, 2017, 44(3):87-93.
[21]	REDDY M R, MANGRAUTHIA S K, REDDY S V, et al.PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII[J]. Journal of Advanced Research, 2020, 30:197-211.
[22]	ZIVCAK M, BRESTIC M, KALAJI H M, et al. Photosynthetic responses of Sun-and shade-grown barley leaves to high light:is the lower PSII connectivity in shade leaves associated with protection against excess of light?[J]. Photosynthesis Research, 2014, 119(3):339-354.
[23]	韩全辉,魏云霞,黄洁.木薯/花生间作体系中木薯行距配置对薯块产量的影响[J]. 江西农业学报, 2017,29(7):1-4.
[24]	COUÉE I, SULMON C, GOUESBET G, et al. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants[J]. Journal of Experimental Botany, 2006, 57(3):449-459.
[25]	DE PASCALI M, VERGINE M, NEGRO C, et al. Xylella fastidiosa and drought stress in olive trees:a complex relationship mediated by soluble sugars[J]. Biology,2022, 11(1):112.
[26]	SINGH N, MISHRA A, JHA B. Ectopic over-expression of peroxisomal ascorbate peroxidase(SbpAPX) gene confers salt stress tolerance in transgenic peanut(Arachis hypogaea)[J]. Gene, 2014, 547(1):119-125.
[27]	ZHANG Z, ZHANG Q, WU J, et al. Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses[J]. PLoS One,2013, 8(2):e57472.
[28]	BONETTA V R. The structure-function relationships and physiological roles of MnSOD mutants[J]. Bioscience Reports, 2022, 42(6):BSR20220202.
[29]	ODOCH M, BUYS E M, TAYLOR J R N. Mechanism of cassava tuber cell wall weakening by dilute sodium hydroxide steeping[J]. Food Chemistry, 2017, 228:338-347.
[30]	SATO Y, MURAKAMI T, FUNATSUKI H, et al. Heat shock-mediated APX gene expression and protection against chilling injury in rice seedlings[J]. Journal of Experimental Botany, 2001, 52(354):145-151.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis of starch accumulation in root tuber of cassava cultivars during different harvesting periods

1. Guangzhou Experimental Station, CATAS, Guangzhou, Guangdong 510140;

2. Tropical Crops Genetic Resources Institute, CATAS, Haikou, Hainan 571737, China

Received Date: 2024-03-26

Rev Recd Date: 2024-10-29

Key words:

cassava /
yield /
starch /
photosynthesis

Abstract: In order to analyze the law of starch accumulation in root tubers of cassava cultivars in different harvesting periods, 4 cassava cultivars ‘SC205’, ‘SC8’, ‘SC9’ and ‘SC6068’ were determined in terms of fresh weight and biomass of root tubers and ratio of amylose and amylopectin in starch after 6 to 10 months of planting,and the underlying mechanism was analyzed. The results showed that there were significant differences in fresh tuber weight in different cassava harvesting periods. There were significant differences in amylose ratio in different harvesting periods in the same cultivar, and the amylose ratio increased in the later growth period of cassava. The ribulose diphosphate carboxylase（Rubisco） content of high-yielding cultivars SC205 and SC8 was generally lower than that of low-yielding cultivars ‘SC6068’ and ‘SC9’ after 7 to 10 months of planting. At 7 to 10 months after planting, the content of PSⅡ oxygen releasing complex gradually increased in SC8, was relatively stable in SC205, and gradually decreased in SC9 and SC6068 at 9 to 10 months after planting. In October cassava production was the highest, and cultivars ‘SC205’, ‘SC9’ and ‘SC6068’ were the highest in the content of PSⅡoxygen releasing complex. The cultivars ‘SC205’, ‘SC8’,‘SC9’ and ‘SC6068’ showed an increasing trend in the content of sucrose phosphate synthetase（SPS） after October, whereas the activity of β-amylase increased from September to October, and decreased from November to December. The protein content of ascorbate peroxidase（APX） was relatively stable in ‘SC6068’ from September to December. The cultivars ‘SC205’, ‘SC8’ and ‘SC9’ showed an increasing trend in APX content from September to December. In conclusion, there were significant differences in yield and starch composition among different cultivars, the mechanism of which was closely related to leaf photosynthesis, antioxidant activity and root starch synthetase activity.

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Reference (30)

[1]	HOANG N H, LE THANH T, THEPBANDIT W, et al.Efficacy of chitosan nanoparticle loaded-salicylic acid and-silver on management of cassava leaf spot disease[J]. Polymers, 2022, 14(4):660.
[2]	苏必孟,刘子凡,黄洁,等.基于主成分分析的木薯抗旱栽培措施的综合评价[J]. 热带作物学报, 2017, 38(2):189-193.
[3]	CARVALHO L J C B, DE SOUZA C R B, DE CASCARDO J C M, et al. Identification and characterization of a novel cassava(Manihot esculenta Crantz)clone with high free sugar content and novel starch[J]. Plant Molecular Biology, 2004, 56(4):643-659.
[4]	WANG Y J, LU X H, ZHEN X H, et al. A transformation and genome editing system for cassava cultivar SC8[J]. Genes, 2022, 13(9):1650.
[5]	罗兴录.不同植物生长调节剂对木薯生长发育和淀粉积累影响的研究[J]. 中国农学通报, 2002, 18(3):30-33.
[6]	罗兴录,劳天源.木薯不同时期施肥对产量和淀粉积累影响研究[J]. 耕作与栽培, 2000,(03):8-9.
[7]	黄洁,林雄,李开绵,等.木薯施肥效应研究[J]. 广西热作科技, 2000(3):1-3.
[8]	罗兴录,岑忠用,谢和霞,等.不同木薯品种抗衰老生理与淀粉积累特性研究[J]. 作物学报, 2007, 33(6):1018-1024.
[9]	HE S, HAO X, WANG S, et al. Starch synthase II plays a crucial role in starch biosynthesis and the formation of multienzyme complexes in cassava storage roots[J]. Journal of Experimental Botany, 2022, 73(8):2540-2557.
[10]	SAITHONG T, RONGSIRIKUL O, KALAPANULAK S,et al. Starch biosynthesis in cassava:a genome-based pathway reconstruction and its exploitation in data integration[J]. BMC Systems Biology, 2013, 7:75.
[11]	HAN J, GU L, WEN J, et al. Inference of photosynthetic capacity parameters from chlorophyll a fluorescence is affected by redox state of PSII reaction centers[J]. Plant,Cell&Environment, 2022, 45(4):1298-1314.
[12]	ECKARDT N A. From the archives:Photosynthesis matters; PSII antenna size, photorespiration, and the evolution of C4 photosynthesis[J]. The Plant Cell, 2022, 34(4):1145-1146.
[13]	PANPETCH P, FIELD R A, LIMPASENI T. Cloning of the full-length isoamylase3 gene from cassava Manihot esculenta Crantz ‘KU50’ and its heterologous expression in E. coli[J]. Plant Physiology and Biochemistry:PPB,2018, 132:281-286.
[14]	UARROTA V G, MORESCO R, SCHMIDT E C, et al.The role of ascorbate peroxidase, guaiacol peroxidase,and polysaccharides in cassava(Manihot esculenta Crantz)roots under postharvest physiological deterioration[J]. Food Chemistry, 2016, 197(Pt A):737-746.
[15]	平吉成,单守明,刘晶,等.库源关系对草莓叶片光合磷酸化和Rubisco活性的影响[J]. 北方园艺, 2011(19):1-4.
[16]	SUGANAMI M, SUZUKI Y, TAZOE Y, et al. Cooverproducing Rubisco and Rubisco activase enhances photosynthesis in the optimal temperature range in rice[J]. Plant Physiology, 2021, 185(1):108-119.
[17]	黄洁,周建国,林世欣,等.对胶园间作木薯的思考及展望[J]. 江西农业学报, 2014, 26(1):64-68.
[18]	罗兴录,陈会鲜,杨鑫,等.木薯不同灌溉方式对产量和土壤理化性状影响研究[J]. 中国农学通报, 2014,30(18):151-155.
[19]	ŚLESAK I, MAZUR Z,ŚLESAK H. Genes encoding the photosystemⅡproteins are under purifying selection:an insight into the early evolution of oxygenic photosynthesis[J]. Photosynthesis Research, 2022, 153(3):163-175.
[20]	罗兴录,周贵靖,朱艳梅,等.药肥施用量对土壤养分和木薯产量的影响[J]. 广东农业科学, 2017, 44(3):87-93.
[21]	REDDY M R, MANGRAUTHIA S K, REDDY S V, et al.PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII[J]. Journal of Advanced Research, 2020, 30:197-211.
[22]	ZIVCAK M, BRESTIC M, KALAJI H M, et al. Photosynthetic responses of Sun-and shade-grown barley leaves to high light:is the lower PSII connectivity in shade leaves associated with protection against excess of light?[J]. Photosynthesis Research, 2014, 119(3):339-354.
[23]	韩全辉,魏云霞,黄洁.木薯/花生间作体系中木薯行距配置对薯块产量的影响[J]. 江西农业学报, 2017,29(7):1-4.
[24]	COUÉE I, SULMON C, GOUESBET G, et al. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants[J]. Journal of Experimental Botany, 2006, 57(3):449-459.
[25]	DE PASCALI M, VERGINE M, NEGRO C, et al. Xylella fastidiosa and drought stress in olive trees:a complex relationship mediated by soluble sugars[J]. Biology,2022, 11(1):112.
[26]	SINGH N, MISHRA A, JHA B. Ectopic over-expression of peroxisomal ascorbate peroxidase(SbpAPX) gene confers salt stress tolerance in transgenic peanut(Arachis hypogaea)[J]. Gene, 2014, 547(1):119-125.
[27]	ZHANG Z, ZHANG Q, WU J, et al. Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses[J]. PLoS One,2013, 8(2):e57472.
[28]	BONETTA V R. The structure-function relationships and physiological roles of MnSOD mutants[J]. Bioscience Reports, 2022, 42(6):BSR20220202.
[29]	ODOCH M, BUYS E M, TAYLOR J R N. Mechanism of cassava tuber cell wall weakening by dilute sodium hydroxide steeping[J]. Food Chemistry, 2017, 228:338-347.
[30]	SATO Y, MURAKAMI T, FUNATSUKI H, et al. Heat shock-mediated APX gene expression and protection against chilling injury in rice seedlings[J]. Journal of Experimental Botany, 2001, 52(354):145-151.

Message Board

Analysis of starch accumulation in root tuber of cassava cultivars during different harvesting periods

doi: 10.15886/j.cnki.rdswxb.20240051

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

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