Construction of a mouse model of allergic asthma induced by fusion of allergen Fel d 1-Can f 1 of cats and dogs

WEI Chunjie; XU Linhao; GAO Jiangrui; ZHENG Anqi; ZHU Yikun; LIANG Xinglan; MAI Yanqi; WANG Dayong; PEI Yechun

doi:10.15886/j.cnki.rdswxb.20240036

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Volume 15 Issue 5

Sep. 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Tropical Biology > 2024 > 15(5): 558-566

WEI Chunjie, XU Linhao, GAO Jiangrui, ZHENG Anqi, ZHU Yikun, LIANG Xinglan, MAI Yanqi, WANG Dayong, PEI Yechun. Construction of a mouse model of allergic asthma induced by fusion of allergen Fel d 1-Can f 1 of cats and dogs[J]. Journal of Tropical Biology, 2024, 15(5): 558-566. doi: 10.15886/j.cnki.rdswxb.20240036

Citation:

WEI Chunjie, XU Linhao, GAO Jiangrui, ZHENG Anqi, ZHU Yikun, LIANG Xinglan, MAI Yanqi, WANG Dayong, PEI Yechun. Construction of a mouse model of allergic asthma induced by fusion of allergen Fel d 1-Can f 1 of cats and dogs[J]. Journal of Tropical Biology, 2024, 15(5): 558-566. doi: 10.15886/j.cnki.rdswxb.20240036

Construction of a mouse model of allergic asthma induced by fusion of allergen Fel d 1-Can f 1 of cats and dogs

doi: 10.15886/j.cnki.rdswxb.20240036

1. School of Life and Health Sciences, Hainan University, Haikou, Hainan 570228, China;
2. School of Pharmaceutical Sciences, Hainan University, Haikou, Hainan 570228, China

Received Date: 2024-03-02
Rev Recd Date: 2024-04-11

Abstract

In order to construct a reliable and stable mouse model of allergic asthma, 12 5-week-old female BALB/c mice were selected and randomly divided into a model group and a control group of 6 mice each. Each mouse in the model group was sensitized with an intraperitoneal injection of 1 mg-mL-1 feline and canine fusion allergen Fel d 1-Can f 1/aluminum hydroxide complexes, and injected with 1 mg-mL-1 feline and canine fusion allergen Fel d 1-Can f 1 via the trachea. The control group was given an equal volume of PBS. A number of indices were employed to ascertain the efficacy of the constructed allergic asthma model, including serum specific IgE levels, airway reactivity levels, allergen-induced stress allergic reactions, ear prick tests, the degree of inflammatory infiltration in lung tissues, the number of polymucous cuprocytes in lung tissues, and the degree of collagen fiber deposition. The results demonstrated that mice exposed to Fel d 1-Can f 1 exhibited a notable elevation in serum-specific IgE levels, a substantial increase in airway responsiveness, a significant decline in allergen-induced body temperature, and an expansion in the area of ear dye. Furthermore, the study revealed that the mice exhibited leakage and a significant enhancement in the net intensity of leakage, as well as a significant increase in inflammatory infiltration of the lungs, an increase in the number of multilocular cup cells, and an aggravation in the degree of collagen fiber deposition, when compared to the control group. In conclusion, a mouse model of allergic asthma was successfully constructed, which will provide model conditions for the development and evaluation of allergy-control vaccines for cats and dogs.
- allergen,
- fusion allergen,
- allergic asthma,
- animal model,
- cat,
- dog

References

[1]	KANG S Y, YANG M S, BORRES M P, et al. The association between specific IgE antibodies to component allergens and allergic symptoms on dog and cat exposure among Korean pet exhibition participants[J]. The World Allergy Organization Journal, 2022, 15(11):100709.
[2]	ZHU H, HUANG Z, LIU T, et al. Sensitization to furry animals in patients with suspected allergic disease in China:a multicenter study[J]. Journal of Asthma and Allergy,2022, 15:1701-1712.
[3]	GRUNDSTRÖM J, NEIMERT-ANDERSSON T, KEMI C,et al. Covalent coupling of vitamin D3 to the major cat allergen Fel d 1 improves the effects of allergen-specific immunotherapy in a mouse model for cat allergy[J]. International Archives of Allergy and Immunology, 2012,157(2):136-146.
[4]	GRÖNLUND H, ADÉDOYIN J, REININGER R, et al.Higher immunoglobulin E antibody levels to recombinant Fel d 1 in cat-allergic children with asthma compared with rhinoconjunctivitis[J]. Clinical and Experimental Allergy:Journal of the British Society for Allergy and Clinical Immunology, 2008, 38(8):1275-1281.
[5]	GRÖNLUND H, SAARNE T, GAFVELIN G, et al. The major cat allergen, Fel d 1, in diagnosis and therapy[J].International Archives of Allergy and Immunology, 2010,151(4):265-274.
[6]	KONIECZNY A, MORGENSTERN J P, BIZINKAUSKAS C B, et al. The major dog allergens, Can f 1 and Can f 2,are salivary lipocalin proteins:cloning and immunological characterization of the recombinant forms[J]. Immunology,1997, 92(4):577-586.
[7]	SCHOU C, SVENDSEN U G, LØWENSTEIN H. Purification and characterization of the major dog allergen, Can f I[J].Clinical and Experimental Allergy:Journal of the British Society for Allergy and Clinical Immunology, 1991, 21(3):321-328.
[8]	陈艳蕾,普晓瑜,陈俊,等.北方地区猫狗毛皮屑致敏特点及变化趋势研究[J].临床耳鼻咽喉头颈外科杂志,2021, 35(4):333-337.
[9]	REININGER R, VARGA E M, ZACH M, et al. Detection of an allergen in dog dander that cross-reacts with the major cat allergen, Fel d 1[J]. Clinical and Experimental Allergy:Journal of the British Society for Allergy and Clinical Immunology, 2007, 37(1):116-124.
[10]	CASARO M, SOUZA V R, OLIVEIRA F A, et al. OVAinduced allergic airway inflammation mouse model[M]//Pre-Clinical Models. New York:Humana Press, 2019:297-301.
[11]	罗承慧,高江瑞,陈俊威,等.尘螨诱导特应性皮炎小鼠模型和哮喘小鼠模型的构建[J].畜牧兽医学报,2024, 55(3):1257-1267.
[12]	李晓烨,肖正泮,刘楚,等.热带螨重组Blo t 5变应原诱导小鼠过敏模型的建立[J].中国畜牧兽医, 2017,44(7):2171-2177.
[13]	PRANGTAWORN P, MAHASONGKRAM K, SAEUNG A, et al. A component-resolved therapeutic vaccine for cockroach allergy made of per a 9 and transforming growth factor-β homologue, an immunosuppressive protein of Brugia malayi[J]. Frontiers in Immunology, 2021,12:676558.
[14]	BATES J H T, RINCON M, IRVIN C G. Animal models of asthma[J]. American Journal of Physiology Lung Cellular and Molecular Physiology, 2009, 297(3):L401-L410.
[15]	钟文清,谭建新.不同佐剂和激发方式对哮喘小鼠肺部炎性反应的影响[J].重庆医学, 2013, 42(36):4433-4435.
[16]	MOELLER A, ASK K, WARBURTON D, et al. The bleomycin animal model:a useful tool to investigate treatment options for idiopathic pulmonary fibrosis?[J]. The International Journal of Biochemistry&Cell Biology,2008, 40(3):362-382.
[17]	LIAO E C, HO C M, YIN S C, et al. Immune responses to Tyrophagus putrescentiae-induced airway inflammation in mice[J]. Journal of Investigational Allergology&Clinical Immunology, 2013, 23(1):20-29.
[18]	李国平,刘志刚,钟南山.重组Derp2变应原诱导小鼠变态反应气道炎症动物模型的建立[J].中华微生物学和免疫学杂志, 2005, 25(7):564-569.
[19]	KIANMEHER M, GHORANI V, BOSKABADY M H.Animal model of asthma, various methods and measured parameters:a methodological review[J]. Iranian Journal of Allergy, Asthma, and Immunology, 2016, 15(6):445-465.
[20]	PEI Y, XIAO Z, WEI S, et al. Studies on HBcAg-rBlo t5-21 fusion protein vaccine that alleviates Blomia tropicalis airway inflammation[J]. Journal of Inflammation Research, 2022, 15:6343-6355.
[21]	NAKATSUJI M, SUGIURA K, SUDA K, et al. Structurebased prediction of the IgE epitopes of the major dog allergen Can f 1[J]. The FEBS Journal, 2022, 289(6):1668-1679.
[22]	SAARNE T, NEIMERT-ANDERSSON T, GRÖNLUND H, et al. Treatment with a Fel d 1 hypoallergen reduces allergic responses in a mouse model for cat allergy[J].Allergy, 2011, 66(2):255-263.
[23]	BAO C, OUYANG C, SHENG H, et al. A mast cellthermoregulatory neuron circuit axis regulates hypothermia in anaphylaxis[J]. Science Immunology, 2023, 8(81):eadc9417.
[24]	PEI Y, GENG S, LIU L, et al. Fel d 1-airway inflammation prevention and treatment by co-immunization vaccine via induction of CD4+CD25-Foxp3+Treg cells[J].Human Vaccines&Immunotherapeutics, 2013, 9(5):1019-1031.
[25]	何胜东,赖克方,姚卫民,等.小鼠哮喘模型气道反应性检测方法的建立[J].广东医学, 2006, 27(11):1659-1661.
[26]	NGUYEN T V, PIAO C H, FAN Y J, et al. Artemisia gmelinii extract alleviates allergic airway inflammation via balancing TH1/TH2 homeostasis and inhibiting mast cell degranulation[J]. International Journal of Molecular Sciences, 2022, 23(23):15377.

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(18) PDF downloads(0) Cited by()

Proportional views

Construction of a mouse model of allergic asthma induced by fusion of allergen Fel d 1-Can f 1 of cats and dogs

1. School of Life and Health Sciences, Hainan University, Haikou, Hainan 570228, China;

2. School of Pharmaceutical Sciences, Hainan University, Haikou, Hainan 570228, China

Received Date: 2024-03-02

Rev Recd Date: 2024-04-11

Key words:

Abstract: In order to construct a reliable and stable mouse model of allergic asthma, 12 5-week-old female BALB/c mice were selected and randomly divided into a model group and a control group of 6 mice each. Each mouse in the model group was sensitized with an intraperitoneal injection of 1 mg-mL-1 feline and canine fusion allergen Fel d 1-Can f 1/aluminum hydroxide complexes, and injected with 1 mg-mL-1 feline and canine fusion allergen Fel d 1-Can f 1 via the trachea. The control group was given an equal volume of PBS. A number of indices were employed to ascertain the efficacy of the constructed allergic asthma model, including serum specific IgE levels, airway reactivity levels, allergen-induced stress allergic reactions, ear prick tests, the degree of inflammatory infiltration in lung tissues, the number of polymucous cuprocytes in lung tissues, and the degree of collagen fiber deposition. The results demonstrated that mice exposed to Fel d 1-Can f 1 exhibited a notable elevation in serum-specific IgE levels, a substantial increase in airway responsiveness, a significant decline in allergen-induced body temperature, and an expansion in the area of ear dye. Furthermore, the study revealed that the mice exhibited leakage and a significant enhancement in the net intensity of leakage, as well as a significant increase in inflammatory infiltration of the lungs, an increase in the number of multilocular cup cells, and an aggravation in the degree of collagen fiber deposition, when compared to the control group. In conclusion, a mouse model of allergic asthma was successfully constructed, which will provide model conditions for the development and evaluation of allergy-control vaccines for cats and dogs.

HTML

Reference (26)

[1]	KANG S Y, YANG M S, BORRES M P, et al. The association between specific IgE antibodies to component allergens and allergic symptoms on dog and cat exposure among Korean pet exhibition participants[J]. The World Allergy Organization Journal, 2022, 15(11):100709.
[2]	ZHU H, HUANG Z, LIU T, et al. Sensitization to furry animals in patients with suspected allergic disease in China:a multicenter study[J]. Journal of Asthma and Allergy,2022, 15:1701-1712.
[3]	GRUNDSTRÖM J, NEIMERT-ANDERSSON T, KEMI C,et al. Covalent coupling of vitamin D3 to the major cat allergen Fel d 1 improves the effects of allergen-specific immunotherapy in a mouse model for cat allergy[J]. International Archives of Allergy and Immunology, 2012,157(2):136-146.
[4]	GRÖNLUND H, ADÉDOYIN J, REININGER R, et al.Higher immunoglobulin E antibody levels to recombinant Fel d 1 in cat-allergic children with asthma compared with rhinoconjunctivitis[J]. Clinical and Experimental Allergy:Journal of the British Society for Allergy and Clinical Immunology, 2008, 38(8):1275-1281.
[5]	GRÖNLUND H, SAARNE T, GAFVELIN G, et al. The major cat allergen, Fel d 1, in diagnosis and therapy[J].International Archives of Allergy and Immunology, 2010,151(4):265-274.
[6]	KONIECZNY A, MORGENSTERN J P, BIZINKAUSKAS C B, et al. The major dog allergens, Can f 1 and Can f 2,are salivary lipocalin proteins:cloning and immunological characterization of the recombinant forms[J]. Immunology,1997, 92(4):577-586.
[7]	SCHOU C, SVENDSEN U G, LØWENSTEIN H. Purification and characterization of the major dog allergen, Can f I[J].Clinical and Experimental Allergy:Journal of the British Society for Allergy and Clinical Immunology, 1991, 21(3):321-328.
[8]	陈艳蕾,普晓瑜,陈俊,等.北方地区猫狗毛皮屑致敏特点及变化趋势研究[J].临床耳鼻咽喉头颈外科杂志,2021, 35(4):333-337.
[9]	REININGER R, VARGA E M, ZACH M, et al. Detection of an allergen in dog dander that cross-reacts with the major cat allergen, Fel d 1[J]. Clinical and Experimental Allergy:Journal of the British Society for Allergy and Clinical Immunology, 2007, 37(1):116-124.
[10]	CASARO M, SOUZA V R, OLIVEIRA F A, et al. OVAinduced allergic airway inflammation mouse model[M]//Pre-Clinical Models. New York:Humana Press, 2019:297-301.
[11]	罗承慧,高江瑞,陈俊威,等.尘螨诱导特应性皮炎小鼠模型和哮喘小鼠模型的构建[J].畜牧兽医学报,2024, 55(3):1257-1267.
[12]	李晓烨,肖正泮,刘楚,等.热带螨重组Blo t 5变应原诱导小鼠过敏模型的建立[J].中国畜牧兽医, 2017,44(7):2171-2177.
[13]	PRANGTAWORN P, MAHASONGKRAM K, SAEUNG A, et al. A component-resolved therapeutic vaccine for cockroach allergy made of per a 9 and transforming growth factor-β homologue, an immunosuppressive protein of Brugia malayi[J]. Frontiers in Immunology, 2021,12:676558.
[14]	BATES J H T, RINCON M, IRVIN C G. Animal models of asthma[J]. American Journal of Physiology Lung Cellular and Molecular Physiology, 2009, 297(3):L401-L410.
[15]	钟文清,谭建新.不同佐剂和激发方式对哮喘小鼠肺部炎性反应的影响[J].重庆医学, 2013, 42(36):4433-4435.
[16]	MOELLER A, ASK K, WARBURTON D, et al. The bleomycin animal model:a useful tool to investigate treatment options for idiopathic pulmonary fibrosis?[J]. The International Journal of Biochemistry&Cell Biology,2008, 40(3):362-382.
[17]	LIAO E C, HO C M, YIN S C, et al. Immune responses to Tyrophagus putrescentiae-induced airway inflammation in mice[J]. Journal of Investigational Allergology&Clinical Immunology, 2013, 23(1):20-29.
[18]	李国平,刘志刚,钟南山.重组Derp2变应原诱导小鼠变态反应气道炎症动物模型的建立[J].中华微生物学和免疫学杂志, 2005, 25(7):564-569.
[19]	KIANMEHER M, GHORANI V, BOSKABADY M H.Animal model of asthma, various methods and measured parameters:a methodological review[J]. Iranian Journal of Allergy, Asthma, and Immunology, 2016, 15(6):445-465.
[20]	PEI Y, XIAO Z, WEI S, et al. Studies on HBcAg-rBlo t5-21 fusion protein vaccine that alleviates Blomia tropicalis airway inflammation[J]. Journal of Inflammation Research, 2022, 15:6343-6355.
[21]	NAKATSUJI M, SUGIURA K, SUDA K, et al. Structurebased prediction of the IgE epitopes of the major dog allergen Can f 1[J]. The FEBS Journal, 2022, 289(6):1668-1679.
[22]	SAARNE T, NEIMERT-ANDERSSON T, GRÖNLUND H, et al. Treatment with a Fel d 1 hypoallergen reduces allergic responses in a mouse model for cat allergy[J].Allergy, 2011, 66(2):255-263.
[23]	BAO C, OUYANG C, SHENG H, et al. A mast cellthermoregulatory neuron circuit axis regulates hypothermia in anaphylaxis[J]. Science Immunology, 2023, 8(81):eadc9417.
[24]	PEI Y, GENG S, LIU L, et al. Fel d 1-airway inflammation prevention and treatment by co-immunization vaccine via induction of CD4+CD25-Foxp3+Treg cells[J].Human Vaccines&Immunotherapeutics, 2013, 9(5):1019-1031.
[25]	何胜东,赖克方,姚卫民,等.小鼠哮喘模型气道反应性检测方法的建立[J].广东医学, 2006, 27(11):1659-1661.
[26]	NGUYEN T V, PIAO C H, FAN Y J, et al. Artemisia gmelinii extract alleviates allergic airway inflammation via balancing TH1/TH2 homeostasis and inhibiting mast cell degranulation[J]. International Journal of Molecular Sciences, 2022, 23(23):15377.

Message Board

Construction of a mouse model of allergic asthma induced by fusion of allergen Fel d 1-Can f 1 of cats and dogs

doi: 10.15886/j.cnki.rdswxb.20240036

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Related