Cloning and expression analysis of visual genes of <i>Megalurothrips usitatus</i>

XIAN Limin; JIN Haifeng; HOU Qingfang; PENG Xiaoying; NING Hengheng; LI Fen; WU Shaoying

doi:10.15886/j.cnki.rdswxb.20240029

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Volume 15 Issue 5

Sep. 2024

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Article Navigation > Journal of Tropical Biology > 2024 > 15(5): 615-622

XIAN Limin, JIN Haifeng, HOU Qingfang, PENG Xiaoying, NING Hengheng, LI Fen, WU Shaoying. Cloning and expression analysis of visual genes of Megalurothrips usitatus[J]. Journal of Tropical Biology, 2024, 15(5): 615-622. doi: 10.15886/j.cnki.rdswxb.20240029

Citation:

XIAN Limin, JIN Haifeng, HOU Qingfang, PENG Xiaoying, NING Hengheng, LI Fen, WU Shaoying. Cloning and expression analysis of visual genes of Megalurothrips usitatus[J]. Journal of Tropical Biology, 2024, 15(5): 615-622. doi: 10.15886/j.cnki.rdswxb.20240029

Cloning and expression analysis of visual genes of Megalurothrips usitatus

doi: 10.15886/j.cnki.rdswxb.20240029

XIAN Limin^1,2,
JIN Haifeng^1,2,
HOU Qingfang^1,2,
PENG Xiaoying^1,2,
NING Hengheng^1,2,
LI Fen^{1,2
,},
WU Shaoying^{1,2
,}

1. Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China;
2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China

Received Date: 2024-02-19
Rev Recd Date: 2024-02-28

Abstract

Megalurothrips usitatus is an important thrips pest of legume crops, with obvious phototaxis（color）,and the light environment can affect its behavior and physiology. Four visual genes were cloned and their sequence data were uploaded to NCBI for searching. Moreover, the expression changes of the visual genes at different ages and genders were detected by real-time PCR. The results indicate that all four visual genes belong to the G protein coupled receptor family members. There were significant differences in expression levels of four visual genes among different age groups of M. usitatus（P ＜ 0.05）, and their expression levels fluctuated in different age groups. There is a significant difference in the expression level of visual genes between female and male adults（P ＜ 0.05）, and the expression level of visual genes in males is higher than that in females at all ages.This study clarified the molecular characteristics of the visual genes of M. usitatus and revealed the patterns of changes during their development, which has important guiding significance for precision control of thrips in the field using methods such as blue sticky insect plates or changing the light environment.
- Megalurothrips usitatus visual gene,
- structure prediction,
- expression analysis,

References

[1]	潘雪莲,杨磊,金海峰,等.豆大蓟马在海南发生及防治的研究进展[J].热带生物学报, 2021, 12(4):508-513.
[2]	杨磊,邵雨,李芬,等.缨翅目害虫蓟马生物防治的研究进展[J].中国生物防治学报, 2021, 37(3):393-405.
[3]	袁琳琳,潘雪莲,陆容材,等.海南普通大蓟马对拟除虫菊酯药剂抗性监测及钠通道突变检测[J].植物保护,2023, 49(3):60-67.
[4]	唐良德,韩云,吴建辉,等.豆大蓟马室内对不同颜色及光波的趋性反应[J].植物保护, 2015, 41(6):169-172.
[5]	LOPEZ-REYES K, ARMSTRONG K F, VAN TOL R W H M, et al. Colour vision in thrips (Thysanoptera)[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2022,(1862):377.
[6]	金海峰,王朝政,侯清芳,等.普通大蓟马MuRhodopsin基因的全长克隆及生物信息学分析[J].热带生物学报,2023, 14(6):651-659.
[7]	STENBERG J A, ERICSON L. Visual cues override olfactory cues in the host-finding process of the monophagous leaf beetle Altica engstroemi[J]. Entomologia Experimentalis et Applicata, 2007, 125(1):81-88.
[8]	VASCONCELLOS-NETO J, MONTEIRO R F. Inspection and evaluation of host plant by the butterfly Mechanitis lysimnia (Nymph., Ithomiinae) before laying eggs:a mechanism to reduce intraspecific competition[J]. Oecologia, 1993, 95(3):431-438.
[9]	DE IBARRA N H, VOROBYEV M, MENZEL R. Mechanisms, functions and ecology of colour vision in the honeybee[J]. Journal of Comparative Physiology A, Neuroethology, Sensory, Neural, and Behavioral Physiology, 2014,200(6):411-433.
[10]	VAN DER KOOI C J, STAVENGA D G, ARIKAWA K,et al. Evolution of insect color vision:from spectral sensitivity to visual lysimnia (Nymph., Ithomiinae) before laying eggs:a mechanism to reduce intraspecific competition[J]. Oecologia, 1993, 95(3):431-438.
[11]	FRENTIU F D, BERNARD G D, CUEVAS C I, et al.Adaptive evolution of color vision as seen through the eyes of butterflies[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007,104(Suppl 1):8634-8640.
[12]	唐艳红,毕思言,王晓迪,等.昆虫视觉系统和视觉蛋白现状及展望[J].中国生物防治学报, 2023, 39(3):718-730.
[13]	LIÉNARD M A, VALENCIA-MONTOYA W A, PIERCE N E. Molecular advances to study the function, evolution and spectral tuning of arthropod visual opsins[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2022, 377(1862):20210279.
[14]	闫硕.棉铃虫视觉基因的表达模式及功能研究[D].北京:中国农业大学, 2015.
[15]	闫硕,张青文,熊晓菲,等.小地老虎UV视蛋白基因的克隆及序列分析[J].动物学杂志, 2012, 47(1):1-8.
[16]	DE ANDRES-BRAGADO L, SPRECHER S G. Mechanisms of vision in the fruit fly[J]. Current Opinion in Insect Science, 2019, 36:25-32.
[17]	HOU Q F, YUAN L L, JIN H F, et al. Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (Thysanoptera:Thripidae)[J]. Frontiers in Physiology, 2023, 14:1161680.
[18]	TERAKITA A, YAMASHITA T, SHICHIDA Y. Highly conserved glutamic acid in the extracellularⅣ-Ⅴloop in rhodopsins acts as the counterion in retinochrome, a member of the rhodopsin family[J]. Proceedings of the National Academy of Sciences of the United States of America, 2000, 97(26):14263-14267.
[19]	杨小凡,魏国树,马爱红,等.昆虫紫外视觉研究进展[J].植物保护学报, 2022, 49(1):131-145.
[20]	高正辉,王婉强,朱芬.昆虫的视蛋白基因研究进展[J].华中昆虫研究, 2019(1):18-25.
[21]	金海峰,陆容材,巩雪燕,等.不同波长光对普通大蓟马生长发育的影响[J].植物保护学报, 2022, 49(6):1809-1810.
[22]	OAKLEY T H, SPEISER D I. How complexity originates:the evolution of animal eyes[J]. Annual Review of Ecology, Evolution, and Systematics, 2015, 46:237-260.
[23]	MA Z Y, LIU J P, GUO X J. A retinal-binding protein mediates olfactory attraction in the migratory Locusts[J].Insect Biochemistry and Molecular Biology, 2019, 114:103214.
[24]	SOKABE T, CHEN H C, LUO J J, et al. A switch in thermal preference in Drosophila larvae depends on multiple rhodopsins[J]. Cell Reports, 2016, 17(2):336-344.
[25]	LU J B, LI Z D, YE Z X, et al. Long-wave opsin involved in body color plastic development in Nilaparvata lugens[J].BMC Genomics, 2023, 24(1):353.
[26]	程文杰,郑霞林,王攀,等.昆虫趋光的性别差异及其影响因素[J].应用生态学报, 2011, 22(12):3351-3357.
[27]	闫凯莉.普通大蓟马复眼形态结构及其趋光(色)行为研究[D].广州:华南农业大学, 2016.
[28]	潘雪莲,杨磊,袁琳琳,等.豆大蓟马钠离子通道基因克隆及分析[J].热带生物学报, 2022, 13(3):249-258.
[29]	WANG L K, CHEN J W, ZHAO C Y, et al. Production and quality of Hami melon (Cucumis melo var. reticulatus) and pest population of Thrips palmi in UV-blocking film greenhouses[J]. Pest Management Science, 2023,79(10):4011-4017.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and expression analysis of visual genes of Megalurothrips usitatus

1. Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China;

2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China

Received Date: 2024-02-19

Rev Recd Date: 2024-02-28

Key words:

Abstract: Megalurothrips usitatus is an important thrips pest of legume crops, with obvious phototaxis（color）,and the light environment can affect its behavior and physiology. Four visual genes were cloned and their sequence data were uploaded to NCBI for searching. Moreover, the expression changes of the visual genes at different ages and genders were detected by real-time PCR. The results indicate that all four visual genes belong to the G protein coupled receptor family members. There were significant differences in expression levels of four visual genes among different age groups of M. usitatus（P ＜ 0.05）, and their expression levels fluctuated in different age groups. There is a significant difference in the expression level of visual genes between female and male adults（P ＜ 0.05）, and the expression level of visual genes in males is higher than that in females at all ages.This study clarified the molecular characteristics of the visual genes of M. usitatus and revealed the patterns of changes during their development, which has important guiding significance for precision control of thrips in the field using methods such as blue sticky insect plates or changing the light environment.

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Reference (29)

[1]	潘雪莲,杨磊,金海峰,等.豆大蓟马在海南发生及防治的研究进展[J].热带生物学报, 2021, 12(4):508-513.
[2]	杨磊,邵雨,李芬,等.缨翅目害虫蓟马生物防治的研究进展[J].中国生物防治学报, 2021, 37(3):393-405.
[3]	袁琳琳,潘雪莲,陆容材,等.海南普通大蓟马对拟除虫菊酯药剂抗性监测及钠通道突变检测[J].植物保护,2023, 49(3):60-67.
[4]	唐良德,韩云,吴建辉,等.豆大蓟马室内对不同颜色及光波的趋性反应[J].植物保护, 2015, 41(6):169-172.
[5]	LOPEZ-REYES K, ARMSTRONG K F, VAN TOL R W H M, et al. Colour vision in thrips (Thysanoptera)[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2022,(1862):377.
[6]	金海峰,王朝政,侯清芳,等.普通大蓟马MuRhodopsin基因的全长克隆及生物信息学分析[J].热带生物学报,2023, 14(6):651-659.
[7]	STENBERG J A, ERICSON L. Visual cues override olfactory cues in the host-finding process of the monophagous leaf beetle Altica engstroemi[J]. Entomologia Experimentalis et Applicata, 2007, 125(1):81-88.
[8]	VASCONCELLOS-NETO J, MONTEIRO R F. Inspection and evaluation of host plant by the butterfly Mechanitis lysimnia (Nymph., Ithomiinae) before laying eggs:a mechanism to reduce intraspecific competition[J]. Oecologia, 1993, 95(3):431-438.
[9]	DE IBARRA N H, VOROBYEV M, MENZEL R. Mechanisms, functions and ecology of colour vision in the honeybee[J]. Journal of Comparative Physiology A, Neuroethology, Sensory, Neural, and Behavioral Physiology, 2014,200(6):411-433.
[10]	VAN DER KOOI C J, STAVENGA D G, ARIKAWA K,et al. Evolution of insect color vision:from spectral sensitivity to visual lysimnia (Nymph., Ithomiinae) before laying eggs:a mechanism to reduce intraspecific competition[J]. Oecologia, 1993, 95(3):431-438.
[11]	FRENTIU F D, BERNARD G D, CUEVAS C I, et al.Adaptive evolution of color vision as seen through the eyes of butterflies[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007,104(Suppl 1):8634-8640.
[12]	唐艳红,毕思言,王晓迪,等.昆虫视觉系统和视觉蛋白现状及展望[J].中国生物防治学报, 2023, 39(3):718-730.
[13]	LIÉNARD M A, VALENCIA-MONTOYA W A, PIERCE N E. Molecular advances to study the function, evolution and spectral tuning of arthropod visual opsins[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2022, 377(1862):20210279.
[14]	闫硕.棉铃虫视觉基因的表达模式及功能研究[D].北京:中国农业大学, 2015.
[15]	闫硕,张青文,熊晓菲,等.小地老虎UV视蛋白基因的克隆及序列分析[J].动物学杂志, 2012, 47(1):1-8.
[16]	DE ANDRES-BRAGADO L, SPRECHER S G. Mechanisms of vision in the fruit fly[J]. Current Opinion in Insect Science, 2019, 36:25-32.
[17]	HOU Q F, YUAN L L, JIN H F, et al. Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (Thysanoptera:Thripidae)[J]. Frontiers in Physiology, 2023, 14:1161680.
[18]	TERAKITA A, YAMASHITA T, SHICHIDA Y. Highly conserved glutamic acid in the extracellularⅣ-Ⅴloop in rhodopsins acts as the counterion in retinochrome, a member of the rhodopsin family[J]. Proceedings of the National Academy of Sciences of the United States of America, 2000, 97(26):14263-14267.
[19]	杨小凡,魏国树,马爱红,等.昆虫紫外视觉研究进展[J].植物保护学报, 2022, 49(1):131-145.
[20]	高正辉,王婉强,朱芬.昆虫的视蛋白基因研究进展[J].华中昆虫研究, 2019(1):18-25.
[21]	金海峰,陆容材,巩雪燕,等.不同波长光对普通大蓟马生长发育的影响[J].植物保护学报, 2022, 49(6):1809-1810.
[22]	OAKLEY T H, SPEISER D I. How complexity originates:the evolution of animal eyes[J]. Annual Review of Ecology, Evolution, and Systematics, 2015, 46:237-260.
[23]	MA Z Y, LIU J P, GUO X J. A retinal-binding protein mediates olfactory attraction in the migratory Locusts[J].Insect Biochemistry and Molecular Biology, 2019, 114:103214.
[24]	SOKABE T, CHEN H C, LUO J J, et al. A switch in thermal preference in Drosophila larvae depends on multiple rhodopsins[J]. Cell Reports, 2016, 17(2):336-344.
[25]	LU J B, LI Z D, YE Z X, et al. Long-wave opsin involved in body color plastic development in Nilaparvata lugens[J].BMC Genomics, 2023, 24(1):353.
[26]	程文杰,郑霞林,王攀,等.昆虫趋光的性别差异及其影响因素[J].应用生态学报, 2011, 22(12):3351-3357.
[27]	闫凯莉.普通大蓟马复眼形态结构及其趋光(色)行为研究[D].广州:华南农业大学, 2016.
[28]	潘雪莲,杨磊,袁琳琳,等.豆大蓟马钠离子通道基因克隆及分析[J].热带生物学报, 2022, 13(3):249-258.
[29]	WANG L K, CHEN J W, ZHAO C Y, et al. Production and quality of Hami melon (Cucumis melo var. reticulatus) and pest population of Thrips palmi in UV-blocking film greenhouses[J]. Pest Management Science, 2023,79(10):4011-4017.

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Cloning and expression analysis of visual genes of Megalurothrips usitatus

doi: 10.15886/j.cnki.rdswxb.20240029

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References

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通讯作者: 陈斌, bchen63@163.com

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Cloning and expression analysis of visual genes of Megalurothrips usitatus

doi: 10.15886/j.cnki.rdswxb.20240029

1. Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China;

2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China

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Cloning and expression analysis of visual genes of Megalurothrips usitatus

doi: 10.15886/j.cnki.rdswxb.20240029

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

Proportional views

Related

Cloning and expression analysis of visual genes of Megalurothrips usitatus

doi: 10.15886/j.cnki.rdswxb.20240029

1. Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China; 2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China

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1. Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China;

2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China