Response of marine eukaryotic microfouling communities to hydrodynamic variation in subtropical Sea

LI Jiancong; CHEN Xin; CHEN Yingjie; LIU Yinyi; TANG Min

doi:10.15886/j.cnki.rdswxb.20240026

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Volume 16 Issue 1

Mar. 2025

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Article Navigation > Journal of Tropical Biology > 2025 > 16(1): 115-124

LI Jiancong, CHEN Xin, CHEN Yingjie, LIU Yinyi, TANG Min. Response of marine eukaryotic microfouling communities to hydrodynamic variation in subtropical Sea[J]. Journal of Tropical Biology, 2025, 16(1): 115-124. doi: 10.15886/j.cnki.rdswxb.20240026

Citation:

LI Jiancong, CHEN Xin, CHEN Yingjie, LIU Yinyi, TANG Min. Response of marine eukaryotic microfouling communities to hydrodynamic variation in subtropical Sea[J]. Journal of Tropical Biology, 2025, 16(1): 115-124. doi: 10.15886/j.cnki.rdswxb.20240026

Response of marine eukaryotic microfouling communities to hydrodynamic variation in subtropical Sea

doi: 10.15886/j.cnki.rdswxb.20240026

1. School of Ecological and Environmental Sciences, Hainan University, Haikou, Hainan 570228, China;
2. School of Physics and Optoelectronic Engineering, Hainan University, Haikou, Hainan 570228, China

Received Date: 2024-02-09
Rev Recd Date: 2024-03-14
Publish Date: 2025-03-15

Abstract

To reveal the impact of hydrodynamic changes on eukaryotic microbial communities in marine fouling,outdoor microcosm experiments were conducted by employing environmental microbial high-throughput sequencing technology to investigate the compositional structure and functional characteristics of eukaryotic microbial communities under different hydrodynamic conditions. There were 146 genus of eukaryotic microorganisms belonging to 18 phylum detected. Clustering analysis reveals significant differences in the microbial community structures between dynamic and static sample groups. The dominant phyla and genera in eukaryotic microbial communities varied under different materials and hydrodynamic conditions. Specifically, in the static group, the phylum Nematoda was dominant, with the genus Mononchus being the most abundant. In the dynamic group the dominant microbial phylum on the surface of the weak hydrodynamic group was Chlorophyta on the materials of fiber reinforced plastics and aluminum alloy, while it was Nematoda in the transferred group.The dominant microbial phylum on the surfaces of the strong hydrodynamic group on all the three materials was Haptophyta, but the community richness and diversity were lower in the strong hydrodynamic group. Cluster analysis revealed significant differences in microbial community structure between dynamic and static sample groups. Moreover, there were differences in metabolic pathways among communities under different hydrodynamic conditions, indicating that changes in hydrodynamic conditions also altered the correlations among species in the eukaryotic microbial community.
- marine eukaryotic microfouling,
- hydrodynamic conditions,
- high-throughput sequencing,
- microcosmic experiment

References

[1]	SONG S, DEMIREL Y K, ATLAR M. An investigation into the effect of biofouling on the ship hydrodynamic characteristics using CFD[J]. Ocean Engineering, 2019, 175:122-137.
[2]	HUNSUCKER K Z, GARDNER H, LIEBERMAN K, et al.Using hydrodynamic testing to assess the performance of fouling control coatings[J]. Ocean Engineering, 2019,194:106677.
[3]	SARKAR P K, PAWAR S S, RATH S K, et al. Antibarnacle biofouling coatings for the protection of marine vessels:synthesis and progress[J]. Environmental Science and Pollution Research, 2022, 29(18):26078-26112.
[4]	DAVIDSON I C, MCCANN L D, FOFONOFF P W, et al.The potential for hull-mediated species transfers by obsolete ships on their final voyages[J]. Diversity and Distributions, 2008, 14(3):518-529.
[5]	GIPPERTH L. The legal design of the international and European Union ban on tributyltin antifouling paint:direct and indirect effects[J]. Journal of Environmental Management, 2009, 90(Suppl 1):S86-S95.
[6]	ROSENHAHN A, SCHILP S, KREUZER H J, et al. The role of"inert"surface chemistry in marine biofouling prevention[J]. Physical Chemistry Chemical Physics, 2010,12(17):4275-4286.
[7]	LI L, LIU W, LIANG T, et al. The adsorption mechanisms of algae-bacteria symbiotic system and its fast formation process[J]. Bioresource Technology, 2020, 315:123854.
[8]	QIAN P Y, LAU S C K, DAHMS H U, et al. Marine biofilms as mediators of colonization by marine macroorganisms:implications for antifouling and aquaculture[J]. Marine Biotechnology, 2007, 9(4):399-410.
[9]	BÖLLMANN J, MARTIENSSEN M. Impact of pH conditions and the characteristics of two electrodialysis membranes on biofilm development under semi-realistic conditions[J]. Biofouling, 2021, 37(9/10):998-1005.
[10]	COUTTS A D M, PIOLA R F, HEWITT C L, et al. Effect of vessel voyage speed on survival of biofouling organisms:implications for translocation of non-indigenous marine species[J]. Biofouling, 2010, 26(1):1-13.
[11]	HUNSUCKER K Z, HUNSUCKER J T, GARDNER H, et al. Static and dynamic comparisons for the evaluation of ship hull coatings[J]. Marine Technology Society Journal, 2017, 51(2):71-75.
[12]	PORTAS A, CARRIOT N, ORTALO-MAGNÉA, et al.Impact of hydrodynamics on community structure and metabolic production of marine biofouling formed in a highly energetic estuary[J]. Marine Environmental Research, 2023, 192:106241.
[13]	OSORIO V, PROIA L, RICART M, et al. Hydrological variation modulates pharmaceutical levels and biofilm responses in a Mediterranean River[J]. The Science of the Total Environment, 2014, 472:1052-1061.
[14]	ROCHEX A, GODON J J, BERNET N, et al. Role of shear stress on composition, diversity and dynamics of biofilm bacterial communities[J]. Water Research, 2008,42(20):4915-4922.
[15]	MURPHY E A K, BARROS J M, SCHULTZ M P, et al.Boundary layer hydrodynamics of patchy biofilms[J]. Biofouling, 2022, 38(7):696-714.
[16]	TAHERZADEH D, PICIOREANU C, HORN H. Mass transfer enhancement in moving biofilm structures[J]. Biophysical Journal, 2012, 102(7):1483-1492.
[17]	陈明华,谢良国,付志强,等.丙酮法和热乙醇法测定浮游植物叶绿素a的方法比对[J]. 环境监测管理与技术, 2016, 28(2):46-48.
[18]	PERRIN A, HERBELIN P, JORAND F P A, et al.Design of a rotating disk reactor to assess the colonization of biofilms by free-living amoebae under high shear rates[J]. Biofouling, 2018, 34(4):368-377.
[19]	OWEN J M. Flow and heat transfer in rotating-disc systems[C] //Proceedings of the 1992 International Symposium on Heat Transfer in Turbomachinery.. Marathon,Greece. Connecticut:Begell House, 1994.
[20]	SCHLICHTING H, GERSTEN K. Fundamentals of boundary-layer theory[M] //Boundary-Layer Theory. Berlin, Heidelberg:Springer Berlin Heidelberg, 2016:29-49.
[21]	MATYUGINA E, BELKOVA N, BORZENKO S, et al.Structure and diversity dynamics of microbial communities at day and night:investigation of meromictic Lake Doroninskoe, Transbaikalia, Russia[J]. Journal of Oceanology and Limnology, 2018, 36(6):1978-1992.
[22]	RUNGRASSAMEE W, KLANCHUI A, MAIBUNKAEW S, et al. Bacterial dynamics in intestines of the black tiger shrimp and the Pacific white shrimp during Vibrio harveyi exposure[J]. Journal of Invertebrate Pathology,2016, 133:12-19.
[23]	范立民.吉富罗非鱼养殖池塘微生物群落研究[D]. 南京:南京农业大学, 2015.
[24]	HUNSUCKER J T, HUNSUCKER K Z, GARDNER H, et al. Influence of hydrodynamic stress on the frictional drag of biofouling communities[J]. Biofouling, 2016,32(10):1209-1221.
[25]	HUNSUCKER K Z, KOKA A, LUND G, et al. Diatom community structure on in-service cruise ship hulls[J]. Biofouling, 2014, 30(9):1133-1140.
[26]	STOODLEY P, SAUER K, DAVIES D G, et al. Biofilms as complex differentiated communities[J]. Annual Review of Microbiology, 2002, 56:187-209.
[27]	PAN M, LI H, HAN X, et al. Effects of hydrodynamic conditions on the composition, spatiotemporal distribution of different extracellular polymeric substances and the architecture of biofilms[J]. Chemosphere, 2022, 307(Pt 4):135965.
[28]	THAMES H T, POKHREL D, WILLIS E, et al. Salmonella biofilm formation under fluidic shear stress on different surface materials[J]. Foods, 2023, 12(9):1918.
[29]	ZARGIEL K A, SWAIN G W. Static vs dynamic settlement and adhesion of diatoms to ship hull coatings[J]. Biofouling, 2014, 30(1):115-129.
[30]	MIN W G, RHO H S, KIM Y I, et al. Bathymetric trends of the deep-sea meiobenthos distributed on the continental shelf, slope, and deep floor of the Ulleung Basin, East Sea[J]. Deep Sea Research Part I:Oceanographic Research Papers, 2023, 200:104136.
[31]	BARRANGUET C, VEUGER B, VAN BEUSEKOM S A M, et al. Divergent composition of algal-bacterial biofilms developing under various external factors[J]. European Journal of Phycology, 2005, 40(1):1-8.
[32]	WOODCOCK S, BESEMER K, BATTIN T J, et al. Modelling the effects of dispersal mechanisms and hydrodynamic regimes upon the structure of microbial communities within fluvial biofilms[J]. Environmental Microbiology, 2013, 15(4):1216-1225.
[33]	OLLOS P J, HUCK P M, SLAWSON R M. Factors Affecting biofilm accumulation in Model Distribution Systems[J]. Journal-American Water Works Association, 2003, 95(1):87-97.
[34]	QIAN P Y, CHENG A, WANG R, et al. Marine biofilms:diversity, interactions and biofouling[J]. Nature Reviews Microbiology, 2022, 20:671-684.
[35]	LEIBOLD M A, HOLYOAK M, MOUQUET N, et al. The metacommunity concept:a framework for multi-scale community ecology[J]. Ecology Letters, 2004, 7(7):601-613.
[36]	BÖLLMANN J, MARTIENSSEN M. Impact of pH conditions and the characteristics of two electrodialysis membranes on biofilm development under semi-realistic conditions[J]. Biofouling, 2021, 37(9/10):998-1005.
[37]	HAGER W H, BOES R M. Hydraulic structures:a positive outlook into the future[J]. Journal of Hydraulic Research, 2014, 52(3):299-310.
[38]	MA B, WANG Y, YE S, et al. Earth microbial cooccurrence network reveals interconnection pattern across microbiomes[J]. Microbiome, 2020, 8(1):82.
[39]	LOUCA S, POLZ M F, MAZEL F, et al. Function and functional redundancy in microbial systems[J]. Nature Ecology&Evolution, 2018, 2:936-943.
[40]	SINGER G, BESEMER K, SCHMITT-KOPPLIN P, et al.Physical heterogeneity increases biofilm resource use and its molecular diversity in stream mesocosms[J]. PLoS One, 2010, 5(4):e9988.
[41]	DOUTERELO I, SHARPE R L, BOXALL J B. Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system[J]. Water Research, 2013, 47(2):503-516.
[42]	MAC KENZIE W R, HOXIE N J, PROCTOR M E, et al.A massive outbreak in Milwaukee of cryptosporidium infection transmitted through the public water supply[J]. The New England Journal of Medicine, 1994, 331(3):161-167.
[43]	艾海男,张青,何强,等.重力流排水管道内流态对生物膜菌落结构的影响[J]. 环境工程学报, 2017, 11(5):2845-2850.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Response of marine eukaryotic microfouling communities to hydrodynamic variation in subtropical Sea

1. School of Ecological and Environmental Sciences, Hainan University, Haikou, Hainan 570228, China;

2. School of Physics and Optoelectronic Engineering, Hainan University, Haikou, Hainan 570228, China

Received Date: 2024-02-09

Rev Recd Date: 2024-03-14

Publish Date: 2025-03-15

Key words:

Abstract: To reveal the impact of hydrodynamic changes on eukaryotic microbial communities in marine fouling,outdoor microcosm experiments were conducted by employing environmental microbial high-throughput sequencing technology to investigate the compositional structure and functional characteristics of eukaryotic microbial communities under different hydrodynamic conditions. There were 146 genus of eukaryotic microorganisms belonging to 18 phylum detected. Clustering analysis reveals significant differences in the microbial community structures between dynamic and static sample groups. The dominant phyla and genera in eukaryotic microbial communities varied under different materials and hydrodynamic conditions. Specifically, in the static group, the phylum Nematoda was dominant, with the genus Mononchus being the most abundant. In the dynamic group the dominant microbial phylum on the surface of the weak hydrodynamic group was Chlorophyta on the materials of fiber reinforced plastics and aluminum alloy, while it was Nematoda in the transferred group.The dominant microbial phylum on the surfaces of the strong hydrodynamic group on all the three materials was Haptophyta, but the community richness and diversity were lower in the strong hydrodynamic group. Cluster analysis revealed significant differences in microbial community structure between dynamic and static sample groups. Moreover, there were differences in metabolic pathways among communities under different hydrodynamic conditions, indicating that changes in hydrodynamic conditions also altered the correlations among species in the eukaryotic microbial community.

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Reference (43)

[1]	SONG S, DEMIREL Y K, ATLAR M. An investigation into the effect of biofouling on the ship hydrodynamic characteristics using CFD[J]. Ocean Engineering, 2019, 175:122-137.
[2]	HUNSUCKER K Z, GARDNER H, LIEBERMAN K, et al.Using hydrodynamic testing to assess the performance of fouling control coatings[J]. Ocean Engineering, 2019,194:106677.
[3]	SARKAR P K, PAWAR S S, RATH S K, et al. Antibarnacle biofouling coatings for the protection of marine vessels:synthesis and progress[J]. Environmental Science and Pollution Research, 2022, 29(18):26078-26112.
[4]	DAVIDSON I C, MCCANN L D, FOFONOFF P W, et al.The potential for hull-mediated species transfers by obsolete ships on their final voyages[J]. Diversity and Distributions, 2008, 14(3):518-529.
[5]	GIPPERTH L. The legal design of the international and European Union ban on tributyltin antifouling paint:direct and indirect effects[J]. Journal of Environmental Management, 2009, 90(Suppl 1):S86-S95.
[6]	ROSENHAHN A, SCHILP S, KREUZER H J, et al. The role of"inert"surface chemistry in marine biofouling prevention[J]. Physical Chemistry Chemical Physics, 2010,12(17):4275-4286.
[7]	LI L, LIU W, LIANG T, et al. The adsorption mechanisms of algae-bacteria symbiotic system and its fast formation process[J]. Bioresource Technology, 2020, 315:123854.
[8]	QIAN P Y, LAU S C K, DAHMS H U, et al. Marine biofilms as mediators of colonization by marine macroorganisms:implications for antifouling and aquaculture[J]. Marine Biotechnology, 2007, 9(4):399-410.
[9]	BÖLLMANN J, MARTIENSSEN M. Impact of pH conditions and the characteristics of two electrodialysis membranes on biofilm development under semi-realistic conditions[J]. Biofouling, 2021, 37(9/10):998-1005.
[10]	COUTTS A D M, PIOLA R F, HEWITT C L, et al. Effect of vessel voyage speed on survival of biofouling organisms:implications for translocation of non-indigenous marine species[J]. Biofouling, 2010, 26(1):1-13.
[11]	HUNSUCKER K Z, HUNSUCKER J T, GARDNER H, et al. Static and dynamic comparisons for the evaluation of ship hull coatings[J]. Marine Technology Society Journal, 2017, 51(2):71-75.
[12]	PORTAS A, CARRIOT N, ORTALO-MAGNÉA, et al.Impact of hydrodynamics on community structure and metabolic production of marine biofouling formed in a highly energetic estuary[J]. Marine Environmental Research, 2023, 192:106241.
[13]	OSORIO V, PROIA L, RICART M, et al. Hydrological variation modulates pharmaceutical levels and biofilm responses in a Mediterranean River[J]. The Science of the Total Environment, 2014, 472:1052-1061.
[14]	ROCHEX A, GODON J J, BERNET N, et al. Role of shear stress on composition, diversity and dynamics of biofilm bacterial communities[J]. Water Research, 2008,42(20):4915-4922.
[15]	MURPHY E A K, BARROS J M, SCHULTZ M P, et al.Boundary layer hydrodynamics of patchy biofilms[J]. Biofouling, 2022, 38(7):696-714.
[16]	TAHERZADEH D, PICIOREANU C, HORN H. Mass transfer enhancement in moving biofilm structures[J]. Biophysical Journal, 2012, 102(7):1483-1492.
[17]	陈明华,谢良国,付志强,等.丙酮法和热乙醇法测定浮游植物叶绿素a的方法比对[J]. 环境监测管理与技术, 2016, 28(2):46-48.
[18]	PERRIN A, HERBELIN P, JORAND F P A, et al.Design of a rotating disk reactor to assess the colonization of biofilms by free-living amoebae under high shear rates[J]. Biofouling, 2018, 34(4):368-377.
[19]	OWEN J M. Flow and heat transfer in rotating-disc systems[C] //Proceedings of the 1992 International Symposium on Heat Transfer in Turbomachinery.. Marathon,Greece. Connecticut:Begell House, 1994.
[20]	SCHLICHTING H, GERSTEN K. Fundamentals of boundary-layer theory[M] //Boundary-Layer Theory. Berlin, Heidelberg:Springer Berlin Heidelberg, 2016:29-49.
[21]	MATYUGINA E, BELKOVA N, BORZENKO S, et al.Structure and diversity dynamics of microbial communities at day and night:investigation of meromictic Lake Doroninskoe, Transbaikalia, Russia[J]. Journal of Oceanology and Limnology, 2018, 36(6):1978-1992.
[22]	RUNGRASSAMEE W, KLANCHUI A, MAIBUNKAEW S, et al. Bacterial dynamics in intestines of the black tiger shrimp and the Pacific white shrimp during Vibrio harveyi exposure[J]. Journal of Invertebrate Pathology,2016, 133:12-19.
[23]	范立民.吉富罗非鱼养殖池塘微生物群落研究[D]. 南京:南京农业大学, 2015.
[24]	HUNSUCKER J T, HUNSUCKER K Z, GARDNER H, et al. Influence of hydrodynamic stress on the frictional drag of biofouling communities[J]. Biofouling, 2016,32(10):1209-1221.
[25]	HUNSUCKER K Z, KOKA A, LUND G, et al. Diatom community structure on in-service cruise ship hulls[J]. Biofouling, 2014, 30(9):1133-1140.
[26]	STOODLEY P, SAUER K, DAVIES D G, et al. Biofilms as complex differentiated communities[J]. Annual Review of Microbiology, 2002, 56:187-209.
[27]	PAN M, LI H, HAN X, et al. Effects of hydrodynamic conditions on the composition, spatiotemporal distribution of different extracellular polymeric substances and the architecture of biofilms[J]. Chemosphere, 2022, 307(Pt 4):135965.
[28]	THAMES H T, POKHREL D, WILLIS E, et al. Salmonella biofilm formation under fluidic shear stress on different surface materials[J]. Foods, 2023, 12(9):1918.
[29]	ZARGIEL K A, SWAIN G W. Static vs dynamic settlement and adhesion of diatoms to ship hull coatings[J]. Biofouling, 2014, 30(1):115-129.
[30]	MIN W G, RHO H S, KIM Y I, et al. Bathymetric trends of the deep-sea meiobenthos distributed on the continental shelf, slope, and deep floor of the Ulleung Basin, East Sea[J]. Deep Sea Research Part I:Oceanographic Research Papers, 2023, 200:104136.
[31]	BARRANGUET C, VEUGER B, VAN BEUSEKOM S A M, et al. Divergent composition of algal-bacterial biofilms developing under various external factors[J]. European Journal of Phycology, 2005, 40(1):1-8.
[32]	WOODCOCK S, BESEMER K, BATTIN T J, et al. Modelling the effects of dispersal mechanisms and hydrodynamic regimes upon the structure of microbial communities within fluvial biofilms[J]. Environmental Microbiology, 2013, 15(4):1216-1225.
[33]	OLLOS P J, HUCK P M, SLAWSON R M. Factors Affecting biofilm accumulation in Model Distribution Systems[J]. Journal-American Water Works Association, 2003, 95(1):87-97.
[34]	QIAN P Y, CHENG A, WANG R, et al. Marine biofilms:diversity, interactions and biofouling[J]. Nature Reviews Microbiology, 2022, 20:671-684.
[35]	LEIBOLD M A, HOLYOAK M, MOUQUET N, et al. The metacommunity concept:a framework for multi-scale community ecology[J]. Ecology Letters, 2004, 7(7):601-613.
[36]	BÖLLMANN J, MARTIENSSEN M. Impact of pH conditions and the characteristics of two electrodialysis membranes on biofilm development under semi-realistic conditions[J]. Biofouling, 2021, 37(9/10):998-1005.
[37]	HAGER W H, BOES R M. Hydraulic structures:a positive outlook into the future[J]. Journal of Hydraulic Research, 2014, 52(3):299-310.
[38]	MA B, WANG Y, YE S, et al. Earth microbial cooccurrence network reveals interconnection pattern across microbiomes[J]. Microbiome, 2020, 8(1):82.
[39]	LOUCA S, POLZ M F, MAZEL F, et al. Function and functional redundancy in microbial systems[J]. Nature Ecology&Evolution, 2018, 2:936-943.
[40]	SINGER G, BESEMER K, SCHMITT-KOPPLIN P, et al.Physical heterogeneity increases biofilm resource use and its molecular diversity in stream mesocosms[J]. PLoS One, 2010, 5(4):e9988.
[41]	DOUTERELO I, SHARPE R L, BOXALL J B. Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system[J]. Water Research, 2013, 47(2):503-516.
[42]	MAC KENZIE W R, HOXIE N J, PROCTOR M E, et al.A massive outbreak in Milwaukee of cryptosporidium infection transmitted through the public water supply[J]. The New England Journal of Medicine, 1994, 331(3):161-167.
[43]	艾海男,张青,何强,等.重力流排水管道内流态对生物膜菌落结构的影响[J]. 环境工程学报, 2017, 11(5):2845-2850.

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Response of marine eukaryotic microfouling communities to hydrodynamic variation in subtropical Sea

doi: 10.15886/j.cnki.rdswxb.20240026

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

Proportional views

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