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Volume 16 Issue 1
Mar.  2025
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Article Navigation >  Journal of Tropical Biology  > 2025  >  16(1): 98-106

LI Pengsheng, LIU Jinlin, YANG Ye. Biochemical mechanism of metabolic resistance of Lasiodiplodia theobromae to difenoconazole[J]. Journal of Tropical Biology, 2025, 16(1): 98-106. doi: 10.15886/j.cnki.rdswxb.20240023
Citation: LI Pengsheng, LIU Jinlin, YANG Ye. Biochemical mechanism of metabolic resistance of Lasiodiplodia theobromae to difenoconazole[J]. Journal of Tropical Biology, 2025, 16(1): 98-106. doi: 10.15886/j.cnki.rdswxb.20240023
shu

Biochemical mechanism of metabolic resistance of Lasiodiplodia theobromae to difenoconazole

doi: 10.15886/j.cnki.rdswxb.20240023

  • 1. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan, 571737, China;

  • 2. Sanya Institute of Breeding and Applications, Hainan University, Sanya, Hainan 572025, China

  • Received Date: 2024-02-06
  • Rev Recd Date: 2024-03-12
  • Publish Date: 2025-03-15
  • In order to explore the biochemical mechanism of metabolic resistance of Lasiodiplodia theobromae from mango to difenoconazole, some physiological and biochemical indexes of L. theobromae resistant to difenoconazole were determined. The sensitivity of 23 strains of L. theobromae to difenoconazole was tested by using the mycelial growth rate method. The sensitivity test showed that 9 of the 23 isolates were resistant to difenoconazole, with EC50 values being from 5.61 to 28.70 μg·mL-1 Metabolic enzyme inhibitors, diethyl maleate（DEM）, piperonylbutoxide（PBO） and triphenyl phosphate（TPP） were selected to mix with difenoconazole to determine their synergistic effect. The results indicated that metabolic enzyme inhibitors all had synergistic effect on resistant isolates when treated with the combination of difenoconazole and DEM, PBO or TPP. After difenoconazole treatment, the enzyme activities of cytochrome P450（P450）, glutathione-S-transferase（GST） and carboxylesterase（CarE） were increased. And the enzyme activity of difenoconazole-resistant isolates was significantly higher than sensitive isolates when treated for 12 h. Additionally, UPLC-MS/MS analysis revealed that difenoconazole residual amount of the sensitive isolates increased significantly compared with that of resistant isolates. These results indicate that GST, P450 and CarE may be related to the metabolic resistance of L.theobromae to difenoconazole. The results would provide new insights into the mechanism of pesticide resistance and lay foundation for in-depth analysis of the molecular mechanism of metabolic resistance to difenoconazole.
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Biochemical mechanism of metabolic resistance of Lasiodiplodia theobromae to difenoconazole

doi: 10.15886/j.cnki.rdswxb.20240023
  • 1. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan, 571737, China;
  • 2. Sanya Institute of Breeding and Applications, Hainan University, Sanya, Hainan 572025, China
  • Received Date: 2024-02-06
  • Rev Recd Date: 2024-03-12
  • Publish Date: 2025-03-15

Abstract: In order to explore the biochemical mechanism of metabolic resistance of Lasiodiplodia theobromae from mango to difenoconazole, some physiological and biochemical indexes of L. theobromae resistant to difenoconazole were determined. The sensitivity of 23 strains of L. theobromae to difenoconazole was tested by using the mycelial growth rate method. The sensitivity test showed that 9 of the 23 isolates were resistant to difenoconazole, with EC50 values being from 5.61 to 28.70 μg·mL-1 Metabolic enzyme inhibitors, diethyl maleate（DEM）, piperonylbutoxide（PBO） and triphenyl phosphate（TPP） were selected to mix with difenoconazole to determine their synergistic effect. The results indicated that metabolic enzyme inhibitors all had synergistic effect on resistant isolates when treated with the combination of difenoconazole and DEM, PBO or TPP. After difenoconazole treatment, the enzyme activities of cytochrome P450（P450）, glutathione-S-transferase（GST） and carboxylesterase（CarE） were increased. And the enzyme activity of difenoconazole-resistant isolates was significantly higher than sensitive isolates when treated for 12 h. Additionally, UPLC-MS/MS analysis revealed that difenoconazole residual amount of the sensitive isolates increased significantly compared with that of resistant isolates. These results indicate that GST, P450 and CarE may be related to the metabolic resistance of L.theobromae to difenoconazole. The results would provide new insights into the mechanism of pesticide resistance and lay foundation for in-depth analysis of the molecular mechanism of metabolic resistance to difenoconazole.

LI Pengsheng, LIU Jinlin, YANG Ye. Biochemical mechanism of metabolic resistance of Lasiodiplodia theobromae to difenoconazole[J]. Journal of Tropical Biology, 2025, 16(1): 98-106. doi: 10.15886/j.cnki.rdswxb.20240023
Citation: LI Pengsheng, LIU Jinlin, YANG Ye. Biochemical mechanism of metabolic resistance of Lasiodiplodia theobromae to difenoconazole[J]. Journal of Tropical Biology, 2025, 16(1): 98-106. doi: 10.15886/j.cnki.rdswxb.20240023
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