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Volume 16 Issue 1
Mar.  2025
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Article Navigation >  Journal of Tropical Biology  > 2025  >  16(1): 79-86

ZHANG Chao, WANG Yuzi, WANG Zhaoqi, CHEN Daipeng, ZHENG Li, XIE Changping. Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu[J]. Journal of Tropical Biology, 2025, 16(1): 79-86. doi: 10.15886/j.cnki.rdswxb.20240001
Citation: ZHANG Chao, WANG Yuzi, WANG Zhaoqi, CHEN Daipeng, ZHENG Li, XIE Changping. Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu[J]. Journal of Tropical Biology, 2025, 16(1): 79-86. doi: 10.15886/j.cnki.rdswxb.20240001
shu

Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu

doi: 10.15886/j.cnki.rdswxb.20240001

  • School of Tropical Agriculture and Forestry/Ministry of Education Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Danzhou, Hainan 571737, China

  • Received Date: 2024-01-03
  • Rev Recd Date: 2024-02-13
  • Publish Date: 2025-03-15
  • In order to clarify the pathogen causing the leaf spot disease on Areca catechu and its biological characteristics near Huangzhutang Village, Longgun Town, Wanning City, Hainan Province, leaves infected with the leaf spot disease were collected from the plants of Areca catechu in the arecanut plantation in the village. The pathogen causing the leaf spot disease was isolated by using tissue isolation method and purified, and its pathogenicity was determined. Identification of the pathogen was carried out through morphological characteristics and phylogenetic analysis based on multiple genes（ITS, TEF1, CAL, HIS, and TUB）. The biological characteristics of the pathogen under different culture conditions were also studied. Results showed that the pathogen causing the leaf spot disease on A. catechu was Diaporthe yunnanensis. The optimal conditions for the pathogen growth were found to be PDA medium, 28 ℃, and pH 6, and light condition is favorable for the growth of the pathogen. This study can provide a theoretic basis for further research on the pathogenicity mechanism of the pathogen, in vitro fungicide screening and development of techniques for prevention and control of the pathogen.
  • [1] 黄昭奋,谢柳.槟榔烂果病的病原菌鉴定[J]. 基因组学与应用生物学, 2009, 28(6):1101-1105.
    [2] 孟秀利,宋薇薇,唐庆华,等.槟榔主要病虫害研究进展[J]. 热带作物学报, 2021, 42(11):3055-3065.
    [3] 李晶,谢开泽,张超,等.槟榔新拟盘多毛孢叶斑病菌的鉴定及生物学特性[J]. 热带作物学报, 2023, 44(8):1671-1678.
    [4] 王洪星.海南槟榔黄化相关病原筛选鉴定研究[D]. 海口:海南大学, 2020.
    [5] CAO X R, XU X M, CHE H Y, et al. Eight Colletotrichum species, including a novel species, are associated with Areca palm anthracnose in Hainan, China[J]. Plant Disease, 2020, 104(5):1369-1377.
    [6] 高保森,陈涛,王洪星.槟榔炭疽病病原菌分离鉴定及防治药剂筛选[J]. 分子植物育种, 2023, 21(22):7444-7449.
    [7] 王超,罗霓,郭利军,等.槟榔炭疽病病原菌鉴定及生物学特性研究[J]. 中国热带农业, 2015(6):43-46.
    [8] WANG H, XU L, ZHANG Z, et al. First report of Curvularia pseudobrachyspora causing leaf spots in Areca catechu in China[J]. Plant Disease, 2019, 103(1):150.
    [9] XU G, QIU F, LI X, et al. Diaporthe limonicola causing leaf spot disease on Areca catechu in China[J]. Plant Disease, 2020, 104(6):1869.
    [10] 徐刚.槟榔间座壳叶斑病病原鉴定及其真菌病毒的全基因组克隆和结构分析[D]. 海口:海南大学, 2020.
    [11] 孙广宇,宗兆锋.植物病理学实验技术[M]. 北京:中国农业出版社, 2002:203-207.
    [12] WHITE T J, BRUNS T, LEE S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M] //PCR Protocols. Amsterdam:Elsevier,1990:315-322.
    [13] CARBONE I, KOHN L M. A method for designing primer sets for speciation studies in filamentous ascomycetes[J]. Mycologia, 1999, 91(3):553-556.
    [14] CROUS P W, GROENEWALD J Z, RISÈDE J M, et al.Calonectria species and their Cylindrocladium anamorphs:species with clavate vesicles[J]. Studies in Mycology, 2006, 55(1):213-226.
    [15] GLASS N L, DONALDSON G C. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes[J]. Applied and Environmental Microbiology, 1995, 61(4):1323-1330.
    [16] 李增平.普通植物病理学实验、实习指导[M]. 北京:中国农业出版社, 2017:81.
    [17] GOMES R R, GLIENKE C, VIDEIRA S I R, et al. Diaporthe:a genus of endophytic, saprobic and plant pathogenic fungi[J]. Persoonia, 2013, 31(1):1-41.
    [18] GUO Y S, CROUS P W, BAI Q, et al. High diversity of Diaporthe species associated with pear shoot canker in China[J]. Persoonia, 2020, 45(1):132-162.
    [19] HUANG F, HOU X, DEWDNEY M M, et al. Diaporthe species occurring on citrus in China[J]. Fungal Diversity,2013, 61(1):237-250.
    [20] GUARNACCIA V, GROENEWALD J Z, WOODHALL J,et al. Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe[J]. Persoonia, 2018, 40(1):135-153.
    [21] ROSSMAN A Y, ADAMS G C, CANNON P F, et al. Recommendations of generic names in Diaporthales competing for protection or use[J]. IMA Fungus, 2015, 6(1):145-154.
    [22] HONGSANAN S, NORPHANPHOUN C, SENANAYAKE I C, et al. Annotated notes on Diaporthe species[J]. Mycosphere, 2023, 14(1):918-1189.
    [23] ZHU Y Q, MA C Y, XUE H, et al. Two new species of Diaporthe(Diaporthaceae, Diaporthales) in China[J]. MycoKeys, 2023, 95:209-228.
    [24] GAO Y H, LIU F, DUAN W J, et al. Diaporthe is paraphyletic[J]. IMA Fungus, 2017, 8(1):153-187.
    [25] 张玉杰,莫贱友,李其利,等.杧果间座壳叶斑病病原种类鉴定[C] //彭友良,宋宝安.植物病理科技创新与绿色防控-中国植物病理学会2021年学术年会论文集.北京:中国农业科学技术出版社, 2021:67.
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Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu

doi: 10.15886/j.cnki.rdswxb.20240001
  • School of Tropical Agriculture and Forestry/Ministry of Education Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Danzhou, Hainan 571737, China
  • Received Date: 2024-01-03
  • Rev Recd Date: 2024-02-13
  • Publish Date: 2025-03-15

Abstract: In order to clarify the pathogen causing the leaf spot disease on Areca catechu and its biological characteristics near Huangzhutang Village, Longgun Town, Wanning City, Hainan Province, leaves infected with the leaf spot disease were collected from the plants of Areca catechu in the arecanut plantation in the village. The pathogen causing the leaf spot disease was isolated by using tissue isolation method and purified, and its pathogenicity was determined. Identification of the pathogen was carried out through morphological characteristics and phylogenetic analysis based on multiple genes（ITS, TEF1, CAL, HIS, and TUB）. The biological characteristics of the pathogen under different culture conditions were also studied. Results showed that the pathogen causing the leaf spot disease on A. catechu was Diaporthe yunnanensis. The optimal conditions for the pathogen growth were found to be PDA medium, 28 ℃, and pH 6, and light condition is favorable for the growth of the pathogen. This study can provide a theoretic basis for further research on the pathogenicity mechanism of the pathogen, in vitro fungicide screening and development of techniques for prevention and control of the pathogen.

ZHANG Chao, WANG Yuzi, WANG Zhaoqi, CHEN Daipeng, ZHENG Li, XIE Changping. Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu[J]. Journal of Tropical Biology, 2025, 16(1): 79-86. doi: 10.15886/j.cnki.rdswxb.20240001
Citation: ZHANG Chao, WANG Yuzi, WANG Zhaoqi, CHEN Daipeng, ZHENG Li, XIE Changping. Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu[J]. Journal of Tropical Biology, 2025, 16(1): 79-86. doi: 10.15886/j.cnki.rdswxb.20240001
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