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Volume 16 Issue 1
Mar.  2025
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Article Navigation >  Journal of Tropical Biology  > 2025  >  16(1): 79-86

ZHANG Chao, WANG Yuzi, WANG Zhaoqi, CHEN Daipeng, ZHENG Li, XIE Changping. Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu[J]. Journal of Tropical Biology, 2025, 16(1): 79-86. doi: 10.15886/j.cnki.rdswxb.20240001
Citation: ZHANG Chao, WANG Yuzi, WANG Zhaoqi, CHEN Daipeng, ZHENG Li, XIE Changping. Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu[J]. Journal of Tropical Biology, 2025, 16(1): 79-86. doi: 10.15886/j.cnki.rdswxb.20240001
shu

Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu

doi: 10.15886/j.cnki.rdswxb.20240001

  • School of Tropical Agriculture and Forestry/Ministry of Education Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Danzhou, Hainan 571737, China

  • Received Date: 2024-01-03
  • Rev Recd Date: 2024-02-13
  • Publish Date: 2025-03-15
  • In order to clarify the pathogen causing the leaf spot disease on Areca catechu and its biological characteristics near Huangzhutang Village, Longgun Town, Wanning City, Hainan Province, leaves infected with the leaf spot disease were collected from the plants of Areca catechu in the arecanut plantation in the village. The pathogen causing the leaf spot disease was isolated by using tissue isolation method and purified, and its pathogenicity was determined. Identification of the pathogen was carried out through morphological characteristics and phylogenetic analysis based on multiple genes（ITS, TEF1, CAL, HIS, and TUB）. The biological characteristics of the pathogen under different culture conditions were also studied. Results showed that the pathogen causing the leaf spot disease on A. catechu was Diaporthe yunnanensis. The optimal conditions for the pathogen growth were found to be PDA medium, 28 ℃, and pH 6, and light condition is favorable for the growth of the pathogen. This study can provide a theoretic basis for further research on the pathogenicity mechanism of the pathogen, in vitro fungicide screening and development of techniques for prevention and control of the pathogen.
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    [8] WANG H, XU L, ZHANG Z, et al. First report of Curvularia pseudobrachyspora causing leaf spots in Areca catechu in China[J]. Plant Disease, 2019, 103(1):150.
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    [17] GOMES R R, GLIENKE C, VIDEIRA S I R, et al. Diaporthe:a genus of endophytic, saprobic and plant pathogenic fungi[J]. Persoonia, 2013, 31(1):1-41.
    [18] GUO Y S, CROUS P W, BAI Q, et al. High diversity of Diaporthe species associated with pear shoot canker in China[J]. Persoonia, 2020, 45(1):132-162.
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Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu

doi: 10.15886/j.cnki.rdswxb.20240001
  • School of Tropical Agriculture and Forestry/Ministry of Education Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Danzhou, Hainan 571737, China
  • Received Date: 2024-01-03
  • Rev Recd Date: 2024-02-13
  • Publish Date: 2025-03-15

Abstract: In order to clarify the pathogen causing the leaf spot disease on Areca catechu and its biological characteristics near Huangzhutang Village, Longgun Town, Wanning City, Hainan Province, leaves infected with the leaf spot disease were collected from the plants of Areca catechu in the arecanut plantation in the village. The pathogen causing the leaf spot disease was isolated by using tissue isolation method and purified, and its pathogenicity was determined. Identification of the pathogen was carried out through morphological characteristics and phylogenetic analysis based on multiple genes（ITS, TEF1, CAL, HIS, and TUB）. The biological characteristics of the pathogen under different culture conditions were also studied. Results showed that the pathogen causing the leaf spot disease on A. catechu was Diaporthe yunnanensis. The optimal conditions for the pathogen growth were found to be PDA medium, 28 ℃, and pH 6, and light condition is favorable for the growth of the pathogen. This study can provide a theoretic basis for further research on the pathogenicity mechanism of the pathogen, in vitro fungicide screening and development of techniques for prevention and control of the pathogen.

ZHANG Chao, WANG Yuzi, WANG Zhaoqi, CHEN Daipeng, ZHENG Li, XIE Changping. Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu[J]. Journal of Tropical Biology, 2025, 16(1): 79-86. doi: 10.15886/j.cnki.rdswxb.20240001
Citation: ZHANG Chao, WANG Yuzi, WANG Zhaoqi, CHEN Daipeng, ZHENG Li, XIE Changping. Pathogen identification and biological characteristics of Diaporthe leaf spot disease of Areca catechu[J]. Journal of Tropical Biology, 2025, 16(1): 79-86. doi: 10.15886/j.cnki.rdswxb.20240001
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