Screening of microorganisms promoting rhizosphere growth in dragon fruit

GONG Wenkun; WU Qiaohui; WANG Gang; YANG Jingming; LÜ Rongting; WANG Beibei

doi:10.15886/j.cnki.rdswxb.20230138

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Volume 15 Issue 5

Sep. 2024

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Article Navigation > Journal of Tropical Biology > 2024 > 15(5): 632-638

GONG Wenkun, WU Qiaohui, WANG Gang, YANG Jingming, LÜ Rongting, WANG Beibei. Screening of microorganisms promoting rhizosphere growth in dragon fruit[J]. Journal of Tropical Biology, 2024, 15(5): 632-638. doi: 10.15886/j.cnki.rdswxb.20230138

Citation:

GONG Wenkun, WU Qiaohui, WANG Gang, YANG Jingming, LÜ Rongting, WANG Beibei. Screening of microorganisms promoting rhizosphere growth in dragon fruit[J]. Journal of Tropical Biology, 2024, 15(5): 632-638. doi: 10.15886/j.cnki.rdswxb.20230138

Screening of microorganisms promoting rhizosphere growth in dragon fruit

doi: 10.15886/j.cnki.rdswxb.20230138

School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China

Received Date: 2023-12-12
Rev Recd Date: 2024-05-14

Abstract

A strain of Trichoderma and a strain of Bacillus were screened from the rhizosphere soil of dragon fruit（Hylocereus undulates Britt.＆Rose） using nitrogen fixation, phosphorus solubilization, potassium solubilization,and IAA production media. The growth promoting effect of their fermentation broth on dragon fruit was determined through pot experiments. The results showed that strains F6（Trichoderma ghanense） and B3（Bacillus velezensis） were screened from the rhizosphere soil of dragon fruit, which had the ability to dissolve phosphorus and potassium, fix nitrogen, and produce IAA. Among them, F6 had the highest ability to fix nitrogen and produce IAA, while B3 had the highest ability to dissolve phosphorus and potassium. Inoculation with microbial agents significantly improved the agronomic traits of dragon fruit. The combined microbial agents（B3+F6） had the most significant effect, increasing the fresh weight by 33.09% and the dry weight by 23.11%. Inoculation with microbial agents can improve soil fertility, significantly increase the contents of available phosphorus, available potassium, and organic matter in the soil. Compared with CK treatment, inoculation with a single strain B3 or F6increased the available phosphorus content by 14.49% and 9.9%, the available potassium content by 10.4% and 16.47%, and the alkaline nitrogen content by 25.85% and 14.35%, respectively, whereas the inoculation of the mixed strains B3+F6 increased the contents of available phosphorus, available potassium, and organic matter by 37.02%, 12.64%, and 16.77%, respectively. Correlation analysis between soil physicochemical properties and growth promoting indicators of dragon fruit shows that under the inoculation with the combined microbial agents the content of soil available potassium is significantly positively correlated with the fresh weight and dry weight of dragon fruit above ground, as well as the accumulation of nitrogen, phosphorus, and potassium in dragon fruit. It can hence be concluded that inoculation with the combined microbial agents（B3+F6） has a significant improvement effect on the fresh and dry weight of the above ground parts of dragon fruit, as well as the root system of dragon fruit. The combined microbial agents have a synergistic effect on the accumulation of nutritional elements, nitrogen, phosphorus and potassium, in dragon fruit, and also has the effect of improving soil fertility.
- dragon fruit,
- promoting microorganisms,
- phosphorus solubilization,
- nitrogen fixation,
- potassium solubilization

References

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Screening of microorganisms promoting rhizosphere growth in dragon fruit

School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China

Received Date: 2023-12-12

Rev Recd Date: 2024-05-14

Key words:

Abstract: A strain of Trichoderma and a strain of Bacillus were screened from the rhizosphere soil of dragon fruit（Hylocereus undulates Britt.＆Rose） using nitrogen fixation, phosphorus solubilization, potassium solubilization,and IAA production media. The growth promoting effect of their fermentation broth on dragon fruit was determined through pot experiments. The results showed that strains F6（Trichoderma ghanense） and B3（Bacillus velezensis） were screened from the rhizosphere soil of dragon fruit, which had the ability to dissolve phosphorus and potassium, fix nitrogen, and produce IAA. Among them, F6 had the highest ability to fix nitrogen and produce IAA, while B3 had the highest ability to dissolve phosphorus and potassium. Inoculation with microbial agents significantly improved the agronomic traits of dragon fruit. The combined microbial agents（B3+F6） had the most significant effect, increasing the fresh weight by 33.09% and the dry weight by 23.11%. Inoculation with microbial agents can improve soil fertility, significantly increase the contents of available phosphorus, available potassium, and organic matter in the soil. Compared with CK treatment, inoculation with a single strain B3 or F6increased the available phosphorus content by 14.49% and 9.9%, the available potassium content by 10.4% and 16.47%, and the alkaline nitrogen content by 25.85% and 14.35%, respectively, whereas the inoculation of the mixed strains B3+F6 increased the contents of available phosphorus, available potassium, and organic matter by 37.02%, 12.64%, and 16.77%, respectively. Correlation analysis between soil physicochemical properties and growth promoting indicators of dragon fruit shows that under the inoculation with the combined microbial agents the content of soil available potassium is significantly positively correlated with the fresh weight and dry weight of dragon fruit above ground, as well as the accumulation of nitrogen, phosphorus, and potassium in dragon fruit. It can hence be concluded that inoculation with the combined microbial agents（B3+F6） has a significant improvement effect on the fresh and dry weight of the above ground parts of dragon fruit, as well as the root system of dragon fruit. The combined microbial agents have a synergistic effect on the accumulation of nutritional elements, nitrogen, phosphorus and potassium, in dragon fruit, and also has the effect of improving soil fertility.

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Reference (41)

[1]	王彩霞,谢良商,卢丽兰,等.海南省主栽红心火龙果品种营养成分分析比较[J].热带农业科学, 2018, 38(3):53-56.
[2]	高曼,甘祖于,王维,等.红肉火龙果营养价值及产品研发现状[J].贵州农机化, 2022(1):16-20.
[3]	应姗姗.火龙果皮中果胶提取及改性研究[D].杭州:浙江大学, 2014.
[4]
[5]	STÉPHANE HACQUARD, RUBEN GARRIDO-OTER, ANTONIO GONZÁLEZ, et al. Microbiota and host nutri‐ tion across plant and animal kingdoms[J]. Cell Host Microbe,2015,17(5):603-616.
[6]	黄文茂,易伦,彭思云,等. PGPR复合菌剂对辣椒生长及根际土壤微生物结构的影响[J].中国土壤与肥料, 2020(1):195-201.
[7]	詹寿发,卢丹妮,毛花英,等. 2株溶磷、解钾与产IAA的内生真菌菌株的筛选、鉴定及促生作用研究[J].中国土壤与肥料, 2017(3):142-151.
[8]	张琪,徐思琪,赵学通,等.核桃专用溶磷、解钾、促生长高效功能微生物的筛选与复配[J].中国农学通报, 2020, 36(34):64-70.
[9]	LIU S T, LEE L Y, TAI C Y, et al. Cloning of an Erwinia herbicola gene necessary for gluconic acid production and enhanced mineral phosphate solubilization in Escherichia coli HB101:nucleotide sequence and probable involve‐ ment in biosynthesis of the coenzyme pyrroloquinoline qui‐ none[J]. Journal of Bacteriology, 1992, 174:5814-5819.
[10]	BABU-KHAN S, YEO T C, MARTIN W L, et al. Clon‐ ing of a mineral phosphate-solubilizing gene from pseudo-monas cepacian[J]. Applied and Environmental Microbi‐ ology, 1995, 61:972-978.
[11]	NAILA I, YINGJIE Y, CHENGSHENG Z, et al. Tempo‐ ral dynamics and variation in the alfalfa root nodule and rhi‐ zosphere microbial communities of coastal sand and lawn soil[J]. Journal of Plant Interactions, 2022, 17(1):173-182.
[12]	王瑾,李小坤,鲁剑巍,等.小分子有机酸和阳离子对含钾矿物钾素释放的影响[J].华中农业大学学报, 2013, 32(3):67-71.
[13]	黄群策,陈启锋,李志真.生物固氮研究的前景[J].科技导报, 1999, 17(1):26-29.
[14]	刘小玉.根际促生菌株的筛选及其复合微生物肥料的研制与肥效研究[D].南京:南京农业大学, 2015.
[15]	梁和钦,李富山,张翰君,等.复合微生物有机肥在红心火龙果上的施用效果试验[J].安徽农学通报, 2018, 24(1):54-56.
[16]	MAB KHALIL RAHAD, M ASHRAFUL ISLAM, M ABDUR RAHIM, et al. Effects of rooting media and vari‐ eties on rooting performance of dragon fruit cuttings (Hylocereu sundatus Haw.)[J]. Research in Agriculture Livestock and Fisheries, 2016, 3(1):67-77.
[17]	PRATIBHA R, ANITA S, DEEPTI S, et al. Improvement of phosphorus uptake, phosphorus use efficiency, and grain yield of upland rice (Oryza sativa L.) in response to phosphate-solubilizing bacteria blended with phosphorus fertilizer[J]. Pedosphere, 2022, 32(5):752-763.
[18]	王勇,陈燕琼,温书恒,等.一株溶磷解钾菌的分离筛选与鉴定[J].安徽农业科学, 2019, 47(10):5-9.
[19]	陈弟,李可增,吴琼,等.火龙果根际土壤微生物2种DNA提取方法的比较[J].热带生物学报, 2019, 10(2):184-189.
[20]	徐晓燕,马毅杰.土壤矿物钾的释放及其在植物营养中的意义[J].土壤通报, 2001, 32(4):173-176.
[21]
[22]	鲍士旦.土壤农化分析[M]. 3版.北京:中国农业出版社, 2000:25-270.
[23]	张勇,李多川.耐热真菌五个中国新记录种[J].菌物学报, 2013, 32(1):142-149.
[24]	李坤,洪秀杰,王欣悦,等.贝莱斯芽孢杆菌TC-52的分离鉴定及其对水稻幼苗生长和立枯病的影响[J].江苏农业科学, 2023:1-9.[http://kns.cnki.net/kcms/detail/32.1214.	S.20231213.1647.002.html.]
[25]	刘悦,徐伟慧,王志刚.大豆根际促生菌的筛选鉴定与促生效应[J].浙江农业学报, 2023, 35(12):2775-2784.
[26]	陈多菲,徐畅,刘文佳,等. 3株水稻根际促生菌的筛选鉴定及促生作用研究[J].江苏农业科学, 2023, 51(24):196-202.
[27]	董晓雪,彭国袁,常卓凡,等.西藏土壤芽孢杆菌的分离鉴定及生防促生菌筛选[J].江苏农业科学,2023, 51(23):114-123.
[28]	危潇,黎妍妍,姚经武,等.哈茨木霉WF2菌株鉴定及对烟草黑胫病的防效[J].中南农业科技, 2023, 44(11):12-15.
[29]	陆妍吉,滕菲,姜姝,等. 2种土壤微生物产IAA能力和促生功能的测定[J].安徽农学通报, 2022, 28(1):22-23.
[30]	杨军,刘承武,李霞,等.豆科植物-微生物共生固氮研究进展[J].植物生理学报, 2023, 59(8):1407-1435.
[31]	周丽秀.黄河三角洲滨海盐碱地固氮和复合微生物菌剂制备和应用[D].济南:齐鲁工业大学, 2024:4-8.
[32]	赵欣,王怡霏,王嘉嘉,等.木霉菌对作物及土壤生态环境的影响[J].中国农业科技导报, 2023, 25(11):166-172.
[33]	伍巧慧,龚文坤,刘新月,等.哈密瓜根际耐高温促生菌的筛选及其促生效应研究[J].中国土壤与肥料, 2023(11):221-228.
[34]	任季平,李发康,丁有来,等.木霉T6和青霉K菌株混合培养的溶磷促生效应[J].草原与草坪, 2019, 39(2):73-78.
[35]	王彦佳,胡伯昂,陈佳欣,等. 2株紫花苜蓿解钾菌的筛选鉴定及其对产量和品质的影响[J].草业学报, 2023, 32(12):139-149.
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Screening of microorganisms promoting rhizosphere growth in dragon fruit

doi: 10.15886/j.cnki.rdswxb.20230138

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通讯作者: 陈斌, bchen63@163.com

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