Application of DNA barcoding in rapid identification of <i>Contarinia maculipennis</i> Felt infesting <i>Dendrobium nobile</i>

CAI Bo; ZHAO Chenyang; LI Fen; CHEN Shiming; JIA Jing; LIU Fuxiu; WU Shaoying

doi:10.15886/j.cnki.rdswxb.20230114

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Volume 15 Issue 4

Jul. 2024

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Article Navigation > Journal of Tropical Biology > 2024 > 15(4): 493-498

CAI Bo, ZHAO Chenyang, LI Fen, CHEN Shiming, JIA Jing, LIU Fuxiu, WU Shaoying. Application of DNA barcoding in rapid identification of Contarinia maculipennis Felt infesting Dendrobium nobile[J]. Journal of Tropical Biology, 2024, 15(4): 493-498. doi: 10.15886/j.cnki.rdswxb.20230114

Citation:

CAI Bo, ZHAO Chenyang, LI Fen, CHEN Shiming, JIA Jing, LIU Fuxiu, WU Shaoying. Application of DNA barcoding in rapid identification of Contarinia maculipennis Felt infesting Dendrobium nobile[J]. Journal of Tropical Biology, 2024, 15(4): 493-498. doi: 10.15886/j.cnki.rdswxb.20230114

Application of DNA barcoding in rapid identification of Contarinia maculipennis Felt infesting Dendrobium nobile

doi: 10.15886/j.cnki.rdswxb.20230114

1. Post-Entry Quarantine Station for Tropical Plant, Haikou Customs District, Haikou, Hainan 570311, China;
2. Nanfan School Sanya Nanfan Institute, Hainan University, Sanya, Hainan 572025, China

Received Date: 2023-09-30
Rev Recd Date: 2024-03-27
Publish Date: 2024-07-25

Abstract

Dendrobium nobile has great medicinal value, unique flower shape and rich color, and hence is well favored in the international market and widely planted in tropical and subtropical areas. The larvae of Contarinia maculipennis Felt are hidden in the bracts of D. nobile for infestation, which can cause the bracts to be deformed and discolored, greatly reducing the ornamental value of D. nobile, and hence affecting its economic value. At the same time, the mature larvae of the pest can also drill into the soil to pupate, and can be spread for a long distance through trade of cut flowers and potted soil due to transportation. At present, this pest has caused serious economic losses to the Dendrobium industry in Okinawa, Fukuoka, Miyazaki, Hawaii and other places in Japan.The insects of family Cecidomyidae are traditionally identified mainly through morphology, which identification method is not accurate especially for the eggs and larvae of its similar species. An accurate and rapid identification of C. maculipennis Felt is hence needed to prevent its invasion into other Dendrobium nobile plantation areas in China. DNA barcoding technology was used to successfully identify the samples collected from a China（Sanya） International Orchid Show, Hainan Island as C. maculates Felt, and the morphological characteristics of C.maculates Felt were described in detail, which provided a scientific basis for accurate and rapid identification of C.maculates Felt by relevant quarantine departments.
- Dendrobium nobile,
- Contarinia maculipennis Felt,
- morphological characteristics,
- molecular diagnosis

References

[1]	罗凯,李泽生,白燕冰,等.石斛兰多样性利用及保护现状[J].黑龙江农业科学, 2021(8):85-89.
[2]	GAGNÉR J. Contarinia maculipennis(Diptera:Cecidomyiidae), a polyphagous pest newly reported for North America[J]. Bulletin of Entomological Research, 1995,85(2):209-214.
[3]	UECHI N, YUKAWA J, TOKUDA M, et al. New information on host plants and distribution ranges of an invasive gall midge, Contarinia maculipennis(Diptera:Cecidomyiidae), and its congeners in Japan[J]. Applied Entomology and Zoology, 2011, 46(3):383-389.
[4]	ABE S, KOTAKA N, Takashima A, et al. Host selection and distribution of Dendrobium okinawense, an endangered epiphytic orchid in Yambaru, Japan[J]. Ecological Research, 2018, 33:1069-1073.
[5]	TOKUDA M, YUKAWA J, YASUDA K, et al. Occurrence of Contarinia maculipennis(Diptera:Cecidomyiidae)infesting flower buds of Dendrobium phalaenopsis(Orchidaceae)in greenhouses on Okinawa Island, Japan[J].Applied Entomology and Zoology, 2002, 37(4):583-587.
[6]	KOLESIK P, Gall midges(Diptera:Cecidomyiidae)of Australian cypress-pines, Callitris spp.(Cupressaceae), with descriptions of three new genera and three new species[J].Australian Journal of Entomology, 2000, 39(4):244-255.
[7]	周慧,敖苏,李培征,等.入侵害虫康瘿蚊在海南省的发生危害调查[J].植物检疫, 2015, 29(1):57-60.
[8]	国家市场监督管理总局,国家标准化管理委员会.斑翅康瘿蚊检疫鉴定方法:GB/T 36836-2018[S].北京:中国标准出版社, 2018.
[9]	陈艳,张晓燕.石斛属植物主要害虫及其风险分析[J].武夷科学, 2002, 18:8-14.
[10]	刘忠善,段歌涛,丁元明,等.云南口岸截获危险性害虫康瘿蚊[J].植物检疫, 2009, 23(2):39-41.
[11]	郑文虎,龙琼华.海南三亚口岸国际热带兰花博览会截获植物疫情情况分析[J].植物检疫, 2015, 29(5):74-76.
[12]	Gagné, R J. and Jaschhof M. 2014. A Catalog of the Cecidomyiidae(Diptera)of the World. 3nd Edition. Digital version 2.[EB/OL].(2014-01-01)[2017-12-16]. https://www. ars. usda. gov/ARSUserFiles/80420580/Gagne_2014_World_Cecidomyiidae_Catalog_3rd_Edition.pdf
[13]	EBACH M C, HOLDREGE C. DNA barcoding is no substitute for taxonomy[J]. Nature, 2005, 434(7034):697.
[14]	SCHINDEL D E, MILLER S E. DNA barcoding a useful tool for taxonomists[J]. Nature, 2005, 435(7038):17.
[15]	贾晓旭,陆俊贤,唐修君,等.石岐鸽mtDNA Cytb基因遗传多样性分析[J].中国家禽, 2023, 45(2):117-120.
[16]	邵仕芳,何鹏飞,吴毅歆,等.基于COI基因鉴定云南省43种蝇及其遗传关系分析[J].西南农业学报, 2022,35(2):373-379.
[17]	高凯. NJ进化树构建方法的改进及其应用[D].北京:北京工业大学,2008.
[18]	周慧,李培征,陈施明,等. 6种杀虫剂对康瘿蚊幼虫室内毒力测定及田间药效试验[J].南方农业学报,2015, 46(2):260-264.
[19]	KAMALA I M. Crop diversification for sustainable management of blossom midge(Contarinia maculipennis felt)of Jasmine(Jasminum sambac L.)[J]. Indian Journal of Ecology, 2019, 46(2):371-377.
[20]	ASKEEW R R, HARRIS K M. Chalcidoidea(Hymenoptera)reared from some gall-inducing Cecidomyiidae(Diptera)[J]. Entomologist’s Monthly Magazine, 2007,143(1721/1723):239-240.
[21]	谈静惠,贺生芳,郭澍强,等. DNA条形码技术在植物检疫中的应用探究[J].南方农业, 2019, 13(3):187.
[22]	李凯兵,吴志毅,刘海军,等.美国进境黑胡桃木中截获1种昆虫的分子鉴定[J].贵州农业科学, 2015,43(11):87-89.
[23]	蔡佳,李井干,王富海,等. 6种检疫性菊科杂草籽的形态学和DNA条形码鉴定[J].中国口岸科学技术,2022, 4(9):55-61.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Application of DNA barcoding in rapid identification of Contarinia maculipennis Felt infesting Dendrobium nobile

1. Post-Entry Quarantine Station for Tropical Plant, Haikou Customs District, Haikou, Hainan 570311, China;

2. Nanfan School Sanya Nanfan Institute, Hainan University, Sanya, Hainan 572025, China

Received Date: 2023-09-30

Rev Recd Date: 2024-03-27

Publish Date: 2024-07-25

Key words:

Abstract: Dendrobium nobile has great medicinal value, unique flower shape and rich color, and hence is well favored in the international market and widely planted in tropical and subtropical areas. The larvae of Contarinia maculipennis Felt are hidden in the bracts of D. nobile for infestation, which can cause the bracts to be deformed and discolored, greatly reducing the ornamental value of D. nobile, and hence affecting its economic value. At the same time, the mature larvae of the pest can also drill into the soil to pupate, and can be spread for a long distance through trade of cut flowers and potted soil due to transportation. At present, this pest has caused serious economic losses to the Dendrobium industry in Okinawa, Fukuoka, Miyazaki, Hawaii and other places in Japan.The insects of family Cecidomyidae are traditionally identified mainly through morphology, which identification method is not accurate especially for the eggs and larvae of its similar species. An accurate and rapid identification of C. maculipennis Felt is hence needed to prevent its invasion into other Dendrobium nobile plantation areas in China. DNA barcoding technology was used to successfully identify the samples collected from a China（Sanya） International Orchid Show, Hainan Island as C. maculates Felt, and the morphological characteristics of C.maculates Felt were described in detail, which provided a scientific basis for accurate and rapid identification of C.maculates Felt by relevant quarantine departments.

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Reference (23)

[1]	罗凯,李泽生,白燕冰,等.石斛兰多样性利用及保护现状[J].黑龙江农业科学, 2021(8):85-89.
[2]	GAGNÉR J. Contarinia maculipennis(Diptera:Cecidomyiidae), a polyphagous pest newly reported for North America[J]. Bulletin of Entomological Research, 1995,85(2):209-214.
[3]	UECHI N, YUKAWA J, TOKUDA M, et al. New information on host plants and distribution ranges of an invasive gall midge, Contarinia maculipennis(Diptera:Cecidomyiidae), and its congeners in Japan[J]. Applied Entomology and Zoology, 2011, 46(3):383-389.
[4]	ABE S, KOTAKA N, Takashima A, et al. Host selection and distribution of Dendrobium okinawense, an endangered epiphytic orchid in Yambaru, Japan[J]. Ecological Research, 2018, 33:1069-1073.
[5]	TOKUDA M, YUKAWA J, YASUDA K, et al. Occurrence of Contarinia maculipennis(Diptera:Cecidomyiidae)infesting flower buds of Dendrobium phalaenopsis(Orchidaceae)in greenhouses on Okinawa Island, Japan[J].Applied Entomology and Zoology, 2002, 37(4):583-587.
[6]	KOLESIK P, Gall midges(Diptera:Cecidomyiidae)of Australian cypress-pines, Callitris spp.(Cupressaceae), with descriptions of three new genera and three new species[J].Australian Journal of Entomology, 2000, 39(4):244-255.
[7]	周慧,敖苏,李培征,等.入侵害虫康瘿蚊在海南省的发生危害调查[J].植物检疫, 2015, 29(1):57-60.
[8]	国家市场监督管理总局,国家标准化管理委员会.斑翅康瘿蚊检疫鉴定方法:GB/T 36836-2018[S].北京:中国标准出版社, 2018.
[9]	陈艳,张晓燕.石斛属植物主要害虫及其风险分析[J].武夷科学, 2002, 18:8-14.
[10]	刘忠善,段歌涛,丁元明,等.云南口岸截获危险性害虫康瘿蚊[J].植物检疫, 2009, 23(2):39-41.
[11]	郑文虎,龙琼华.海南三亚口岸国际热带兰花博览会截获植物疫情情况分析[J].植物检疫, 2015, 29(5):74-76.
[12]	Gagné, R J. and Jaschhof M. 2014. A Catalog of the Cecidomyiidae(Diptera)of the World. 3nd Edition. Digital version 2.[EB/OL].(2014-01-01)[2017-12-16]. https://www. ars. usda. gov/ARSUserFiles/80420580/Gagne_2014_World_Cecidomyiidae_Catalog_3rd_Edition.pdf
[13]	EBACH M C, HOLDREGE C. DNA barcoding is no substitute for taxonomy[J]. Nature, 2005, 434(7034):697.
[14]	SCHINDEL D E, MILLER S E. DNA barcoding a useful tool for taxonomists[J]. Nature, 2005, 435(7038):17.
[15]	贾晓旭,陆俊贤,唐修君,等.石岐鸽mtDNA Cytb基因遗传多样性分析[J].中国家禽, 2023, 45(2):117-120.
[16]	邵仕芳,何鹏飞,吴毅歆,等.基于COI基因鉴定云南省43种蝇及其遗传关系分析[J].西南农业学报, 2022,35(2):373-379.
[17]	高凯. NJ进化树构建方法的改进及其应用[D].北京:北京工业大学,2008.
[18]	周慧,李培征,陈施明,等. 6种杀虫剂对康瘿蚊幼虫室内毒力测定及田间药效试验[J].南方农业学报,2015, 46(2):260-264.
[19]	KAMALA I M. Crop diversification for sustainable management of blossom midge(Contarinia maculipennis felt)of Jasmine(Jasminum sambac L.)[J]. Indian Journal of Ecology, 2019, 46(2):371-377.
[20]	ASKEEW R R, HARRIS K M. Chalcidoidea(Hymenoptera)reared from some gall-inducing Cecidomyiidae(Diptera)[J]. Entomologist’s Monthly Magazine, 2007,143(1721/1723):239-240.
[21]	谈静惠,贺生芳,郭澍强,等. DNA条形码技术在植物检疫中的应用探究[J].南方农业, 2019, 13(3):187.
[22]	李凯兵,吴志毅,刘海军,等.美国进境黑胡桃木中截获1种昆虫的分子鉴定[J].贵州农业科学, 2015,43(11):87-89.
[23]	蔡佳,李井干,王富海,等. 6种检疫性菊科杂草籽的形态学和DNA条形码鉴定[J].中国口岸科学技术,2022, 4(9):55-61.

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Application of DNA barcoding in rapid identification of Contarinia maculipennis Felt infesting Dendrobium nobile

doi: 10.15886/j.cnki.rdswxb.20230114

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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Application of DNA barcoding in rapid identification of Contarinia maculipennis Felt infesting Dendrobium nobile

doi: 10.15886/j.cnki.rdswxb.20230114

1. Post-Entry Quarantine Station for Tropical Plant, Haikou Customs District, Haikou, Hainan 570311, China; 2. Nanfan School Sanya Nanfan Institute, Hainan University, Sanya, Hainan 572025, China

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1. Post-Entry Quarantine Station for Tropical Plant, Haikou Customs District, Haikou, Hainan 570311, China;

2. Nanfan School Sanya Nanfan Institute, Hainan University, Sanya, Hainan 572025, China