Genetic diversity of <i>Plesiomyzon baotingensis</i>, a rare and endangered fish in Hainan Province, and its conservation

DENG Shuqing; CAI Xingwei; WANG Han; FU Chenghui; ZHANG Qingfeng; SHEN Zhixin; LI Gaojun; LI Fangyuan

doi:10.15886/j.cnki.rdswxb.20230112

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Volume 15 Issue 4

Jul. 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Tropical Biology > 2024 > 15(4): 419-426

DENG Shuqing, CAI Xingwei, WANG Han, FU Chenghui, ZHANG Qingfeng, SHEN Zhixin, LI Gaojun, LI Fangyuan. Genetic diversity of Plesiomyzon baotingensis, a rare and endangered fish in Hainan Province, and its conservation[J]. Journal of Tropical Biology, 2024, 15(4): 419-426. doi: 10.15886/j.cnki.rdswxb.20230112

Citation:

DENG Shuqing, CAI Xingwei, WANG Han, FU Chenghui, ZHANG Qingfeng, SHEN Zhixin, LI Gaojun, LI Fangyuan. Genetic diversity of Plesiomyzon baotingensis, a rare and endangered fish in Hainan Province, and its conservation[J]. Journal of Tropical Biology, 2024, 15(4): 419-426. doi: 10.15886/j.cnki.rdswxb.20230112

Genetic diversity of Plesiomyzon baotingensis, a rare and endangered fish in Hainan Province, and its conservation

doi: 10.15886/j.cnki.rdswxb.20230112

1. Hainan Academy of Ocean and Fisheries Sciences, Haikou, Hainan 571126, China;
2. The Changjiang Civilization Museum (Wuhan Natural History Museum), Wuhan, Hubei 430010, China;
3. Yinggeling Branch, Hainan Tropic Rainforest National Park Administration, Baisha, Hainan 572800, China;
4. Hainan Research Center for Freshwater Biological Resource and Ecological Environmental Protection Haikou, Hainan 571126, China

Received Date: 2023-09-17
Rev Recd Date: 2023-11-26
Publish Date: 2024-07-25

Abstract

Plesiomyzon baotingensis is endemic to Hainan province. Due to river pollution, overfishing, invasive species and habitat degradation, the wild population of P. baotingensis is quite small in Hainan Province. An attempt was made to estimate genetic diversity of P. baotingensis to provide experimental data of its germplasm resources. An analysis was made of 103 individuals including 5 geographic populations（Bacun, Yinggeling,Qiongzhong, Hongjuntan and Bengling）. Partial sequences of Cytb gene（1 047 bp） were obtained from these individuals. Fifty-eight variable sites and 11 haplotypes were identified in the whole populations, respectively with relatively high haplotype diversity（h=0.815） and high nucleotide diversity（π=0.024 71）. The analysis of molecular variance of the populations indicated that most of genetic variation（77.86%） came from between population. Fst value among five populations was 0.779（P ＜ 0.01）, and the genetic distance between populations ranged from 0.000 3 to 0.046 4; the farthest genetic distance（0.046 4） of P. baotingensis was identified between Bacun and Hongjuntan populations, and the closest genetic distance（0.000 3） was found between Yinggeling and Qiongzhong populations. Fu’s Fs neutral test showed that historical population expansion was observed in the Yinggeling population. Phylogenetic tree showed there were two lineages in the five P. baotingensis populations in Hainan. In order to better conserve the wild resources and genetic structure of P. baotingensis, two mitochondrial DNA lineages should be paid more attention to. The geographical population of P. baotingensis in Bengling was found to contain two lineages, and its haplotypes and endemic haplotypes were the largest in number among the five geographical populations. It is suggested to strengthen the conservation of P. baotingensis in the Bengling area.
- Plesiomyzon baotingensis,
- mitochondrial DNA,
- Cytb gene,
- genetic diversity

References

[1]	蔡杏伟,王裕旭,吴善宇.保亭近原吸鳅[J].生物资源,2019, 41(6):562.
[2]	王群,李东海,杨小波,等.海南霸王岭橡胶林林下植物多样性特征研究[J].林业资源管理, 2022(5):76-83.
[3]	乐佩琦.中国动物志硬骨鱼纲鲤形目:下卷[M].北京:科学出版社, 2000.
[4]	申志新,李高俊,蔡杏伟,等.海南省淡水野生鱼类多样性演变及保护建议[J].中国水产, 2018(11):56-60.
[5]	张鹗,曹文宣.中国生物多样性红色名录:脊椎动物:淡水鱼类[M].北京:科学出版社, 2021.
[6]	李文静,王环珊,刘焕章,等.赤水河半䱗的遗传多样性和种群历史动态分析[J].水生生物学报, 2018, 42(1):106-113.
[7]	夏聪,张雨阳,戴逢斌,等.长江干流水系鲢遗传多样性现状及成因分析[J].中国水产科学, 2022, 29(11):1612-1624.
[8]	俞丹,张智,张健,等.基于Cyt b基因的雅鲁藏布江下游墨脱江段及察隅河墨脱裂腹鱼的遗传多样性及种群历史动态分析[J].水生生物学报, 2019, 43(5):923-930.
[9]	任海,王俊,陆宏芳.恢复生态学的理论与研究进展[J].生态学报, 2014, 34(15):4117-4124.
[10]	彭敏,韩耀全,王大鹏,等.基于线粒体Cytb基因序列的西江流域广西境内卷口鱼遗传多样性分析[J].南方水产科学, 2020, 16(5):10-18.
[11]	ZATTARA E E, PREMOLI A C. Genetic structuring in Andean landlocked populations of Galaxias maculatus:effects of biogeographic history[J]. Journal of Biogeography, 2005, 32(1):5-14.
[12]	李小兵,唐琼英,俞丹,等.基于线粒体细胞色素b基因序列探讨长江流域斑点蛇种群遗传结构和地理分化[J].四川动物, 2018, 37(3):251-259.
[13]	李大命,唐晟凯,刘燕山,等.基于Cytb基因的江苏省大银鱼种群遗传多样性和遗传结构分析[J].上海海洋大学学报, 2021, 30(3):416-425.
[14]	高嘉昕,俞丹,刘焕章.基于cyt b基因的长江中上游大鳞马口鱼遗传多样性及谱系生物地理学过程分析[J].四川动物, 2021, 40(4):361-373.
[15]	XIAO W, ZHANG Y, LIU H. Molecular systematics of Xenocyprinae(Teleostei:Cyprinidae):taxonomy, biogeography, and coevolution of a special group restricted in East Asia[J]. Molecular Phylogenetics and Evolution,2001, 18(2):163-173.
[16]	BURLAND T G. DNASTAR's Lasergene sequence analysis software[J]. Methods in Molecular Biology, 2000,132:71-91.
[17]	LIBRADO P, ROZAS J. DnaSP v5:a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics, 2009, 25(11):1451-1452.
[18]	EXCOFFIER L, LISCHER H E L. Arlequin suite ver 3.5:a new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources, 2010, 10(3):564-567.
[19]	KUMAR S, STECHER G, TAMURA K. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution, 2016, 33(7):1870-1874.
[20]	SHI L X, ZHANG C, WANG Y P, et al. Evolutionary relationships of two balitorids(Cypriniformes, Balitoridae)revealed by comparative mitogenomics[J].Zoologica Scripta, 2018, 47(3):300-310.
[21]	施立明.遗传多样性及其保存[J].生物科学信息,1990, 2(4):158-164.
[22]
[23]	岳丽佳,熊良伟,王帅兵,等.基于线粒体Cytb序列的3个宽体金线蛭群体遗传多样性分析[J].上海海洋大学学报, 2020, 29(1):9-16.
[24]	芦丽.平舟原缨口鳅和海南原缨口鳅的遗传变异和地理分化[D].广州:华南师范大学, 2012.
[25]	GRANT W, BOWEN B. Shallow population histories in deep evolutionary lineages of marine fishes:insights from sardines and anchovies and lessons for conservation[J].Journal of Heredity, 1998, 89(5):415-426.
[26]	WARD R D, WOODWARK M, SKIBINSKI D O F. A comparison of genetic diversity levels in marine, freshwater, and anadromous fishes[J]. Journal of Fish Biology,1994, 44(2):213-232.
[27]	SHAKLEE J B, TAMARU C S, WAPLES R S. Speciation and evolution of marine fishes studied by the electrophoretic analysis of proteins[J]. Pacific Science, 1982, 36(2):141-157.
[28]	WRIGHT S. Evolution and the genetics of populations:Variability within and among populations V. 4[M]. Chicago:University of Chicago Press, 1978.
[29]	MORITZ C. Defining'Evolutionarily Significant Units'for conservation[J]. Trends in Ecology&Evolution,1994, 9(10):373-375.
[30]	ZHOU X, GUANG X, SUN D, et al. Population genomics of finless porpoises reveal an incipient cetacean species adapted to freshwater[J]. Nature Communications, 2018,9:1276.
[31]
[32]	MARIE-STEPHANE T, MIREILLE O, SERGE K. An integrative morphological and molecular diagnostics for Typhlodromus pyri(Acari:Phytoseiidae)[J]. Zoologica Scripta, 2012, 41(1):68-78.
[33]	吴俊颉,李光华,金方彭,等.基于线粒体D-loop区的抚仙湖鱇浪白鱼遗传多样性分析[J].水生生物学报,2022, 46(3):385-394.
[34]	SZÖLLŐSI G J, TANNIER E, DAUBIN V, et al. The inference of gene trees with species trees[J]. Systematic Biology, 2015, 64(1):e42-e62.

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(9) PDF downloads(0) Cited by()

Proportional views

Genetic diversity of Plesiomyzon baotingensis, a rare and endangered fish in Hainan Province, and its conservation

1. Hainan Academy of Ocean and Fisheries Sciences, Haikou, Hainan 571126, China;

2. The Changjiang Civilization Museum (Wuhan Natural History Museum), Wuhan, Hubei 430010, China;

3. Yinggeling Branch, Hainan Tropic Rainforest National Park Administration, Baisha, Hainan 572800, China;

4. Hainan Research Center for Freshwater Biological Resource and Ecological Environmental Protection Haikou, Hainan 571126, China

Received Date: 2023-09-17

Rev Recd Date: 2023-11-26

Publish Date: 2024-07-25

Key words:

Abstract: Plesiomyzon baotingensis is endemic to Hainan province. Due to river pollution, overfishing, invasive species and habitat degradation, the wild population of P. baotingensis is quite small in Hainan Province. An attempt was made to estimate genetic diversity of P. baotingensis to provide experimental data of its germplasm resources. An analysis was made of 103 individuals including 5 geographic populations（Bacun, Yinggeling,Qiongzhong, Hongjuntan and Bengling）. Partial sequences of Cytb gene（1 047 bp） were obtained from these individuals. Fifty-eight variable sites and 11 haplotypes were identified in the whole populations, respectively with relatively high haplotype diversity（h=0.815） and high nucleotide diversity（π=0.024 71）. The analysis of molecular variance of the populations indicated that most of genetic variation（77.86%） came from between population. Fst value among five populations was 0.779（P ＜ 0.01）, and the genetic distance between populations ranged from 0.000 3 to 0.046 4; the farthest genetic distance（0.046 4） of P. baotingensis was identified between Bacun and Hongjuntan populations, and the closest genetic distance（0.000 3） was found between Yinggeling and Qiongzhong populations. Fu’s Fs neutral test showed that historical population expansion was observed in the Yinggeling population. Phylogenetic tree showed there were two lineages in the five P. baotingensis populations in Hainan. In order to better conserve the wild resources and genetic structure of P. baotingensis, two mitochondrial DNA lineages should be paid more attention to. The geographical population of P. baotingensis in Bengling was found to contain two lineages, and its haplotypes and endemic haplotypes were the largest in number among the five geographical populations. It is suggested to strengthen the conservation of P. baotingensis in the Bengling area.

HTML

Reference (34)

[1]	蔡杏伟,王裕旭,吴善宇.保亭近原吸鳅[J].生物资源,2019, 41(6):562.
[2]	王群,李东海,杨小波,等.海南霸王岭橡胶林林下植物多样性特征研究[J].林业资源管理, 2022(5):76-83.
[3]	乐佩琦.中国动物志硬骨鱼纲鲤形目:下卷[M].北京:科学出版社, 2000.
[4]	申志新,李高俊,蔡杏伟,等.海南省淡水野生鱼类多样性演变及保护建议[J].中国水产, 2018(11):56-60.
[5]	张鹗,曹文宣.中国生物多样性红色名录:脊椎动物:淡水鱼类[M].北京:科学出版社, 2021.
[6]	李文静,王环珊,刘焕章,等.赤水河半䱗的遗传多样性和种群历史动态分析[J].水生生物学报, 2018, 42(1):106-113.
[7]	夏聪,张雨阳,戴逢斌,等.长江干流水系鲢遗传多样性现状及成因分析[J].中国水产科学, 2022, 29(11):1612-1624.
[8]	俞丹,张智,张健,等.基于Cyt b基因的雅鲁藏布江下游墨脱江段及察隅河墨脱裂腹鱼的遗传多样性及种群历史动态分析[J].水生生物学报, 2019, 43(5):923-930.
[9]	任海,王俊,陆宏芳.恢复生态学的理论与研究进展[J].生态学报, 2014, 34(15):4117-4124.
[10]	彭敏,韩耀全,王大鹏,等.基于线粒体Cytb基因序列的西江流域广西境内卷口鱼遗传多样性分析[J].南方水产科学, 2020, 16(5):10-18.
[11]	ZATTARA E E, PREMOLI A C. Genetic structuring in Andean landlocked populations of Galaxias maculatus:effects of biogeographic history[J]. Journal of Biogeography, 2005, 32(1):5-14.
[12]	李小兵,唐琼英,俞丹,等.基于线粒体细胞色素b基因序列探讨长江流域斑点蛇种群遗传结构和地理分化[J].四川动物, 2018, 37(3):251-259.
[13]	李大命,唐晟凯,刘燕山,等.基于Cytb基因的江苏省大银鱼种群遗传多样性和遗传结构分析[J].上海海洋大学学报, 2021, 30(3):416-425.
[14]	高嘉昕,俞丹,刘焕章.基于cyt b基因的长江中上游大鳞马口鱼遗传多样性及谱系生物地理学过程分析[J].四川动物, 2021, 40(4):361-373.
[15]	XIAO W, ZHANG Y, LIU H. Molecular systematics of Xenocyprinae(Teleostei:Cyprinidae):taxonomy, biogeography, and coevolution of a special group restricted in East Asia[J]. Molecular Phylogenetics and Evolution,2001, 18(2):163-173.
[16]	BURLAND T G. DNASTAR's Lasergene sequence analysis software[J]. Methods in Molecular Biology, 2000,132:71-91.
[17]	LIBRADO P, ROZAS J. DnaSP v5:a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics, 2009, 25(11):1451-1452.
[18]	EXCOFFIER L, LISCHER H E L. Arlequin suite ver 3.5:a new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources, 2010, 10(3):564-567.
[19]	KUMAR S, STECHER G, TAMURA K. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution, 2016, 33(7):1870-1874.
[20]	SHI L X, ZHANG C, WANG Y P, et al. Evolutionary relationships of two balitorids(Cypriniformes, Balitoridae)revealed by comparative mitogenomics[J].Zoologica Scripta, 2018, 47(3):300-310.
[21]	施立明.遗传多样性及其保存[J].生物科学信息,1990, 2(4):158-164.
[22]
[23]	岳丽佳,熊良伟,王帅兵,等.基于线粒体Cytb序列的3个宽体金线蛭群体遗传多样性分析[J].上海海洋大学学报, 2020, 29(1):9-16.
[24]	芦丽.平舟原缨口鳅和海南原缨口鳅的遗传变异和地理分化[D].广州:华南师范大学, 2012.
[25]	GRANT W, BOWEN B. Shallow population histories in deep evolutionary lineages of marine fishes:insights from sardines and anchovies and lessons for conservation[J].Journal of Heredity, 1998, 89(5):415-426.
[26]	WARD R D, WOODWARK M, SKIBINSKI D O F. A comparison of genetic diversity levels in marine, freshwater, and anadromous fishes[J]. Journal of Fish Biology,1994, 44(2):213-232.
[27]	SHAKLEE J B, TAMARU C S, WAPLES R S. Speciation and evolution of marine fishes studied by the electrophoretic analysis of proteins[J]. Pacific Science, 1982, 36(2):141-157.
[28]	WRIGHT S. Evolution and the genetics of populations:Variability within and among populations V. 4[M]. Chicago:University of Chicago Press, 1978.
[29]	MORITZ C. Defining'Evolutionarily Significant Units'for conservation[J]. Trends in Ecology&Evolution,1994, 9(10):373-375.
[30]	ZHOU X, GUANG X, SUN D, et al. Population genomics of finless porpoises reveal an incipient cetacean species adapted to freshwater[J]. Nature Communications, 2018,9:1276.
[31]
[32]	MARIE-STEPHANE T, MIREILLE O, SERGE K. An integrative morphological and molecular diagnostics for Typhlodromus pyri(Acari:Phytoseiidae)[J]. Zoologica Scripta, 2012, 41(1):68-78.
[33]	吴俊颉,李光华,金方彭,等.基于线粒体D-loop区的抚仙湖鱇浪白鱼遗传多样性分析[J].水生生物学报,2022, 46(3):385-394.
[34]	SZÖLLŐSI G J, TANNIER E, DAUBIN V, et al. The inference of gene trees with species trees[J]. Systematic Biology, 2015, 64(1):e42-e62.

Message Board

Genetic diversity of Plesiomyzon baotingensis, a rare and endangered fish in Hainan Province, and its conservation

doi: 10.15886/j.cnki.rdswxb.20230112

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Related

Genetic diversity of Plesiomyzon baotingensis, a rare and endangered fish in Hainan Province, and its conservation

doi: 10.15886/j.cnki.rdswxb.20230112

HTML

Catalog

Message Board

Genetic diversity of Plesiomyzon baotingensis, a rare and endangered fish in Hainan Province, and its conservation

doi: 10.15886/j.cnki.rdswxb.20230112

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Related

Genetic diversity of Plesiomyzon baotingensis, a rare and endangered fish in Hainan Province, and its conservation

doi: 10.15886/j.cnki.rdswxb.20230112

HTML

Catalog

Export File

Citation

Format

Content